Molecular cloning of a K+ channel from the malaria parasite Plasmodium falciparum

Peter Ellekvist; Christina Høier Ricke; Thomas Litman; Ali Salanti; Hanne Colding; Thomas Zeuthen; Dan A Klaerke

doi:10.1016/j.bbrc.2004.04.049

Molecular cloning of a K⁺ channel from the malaria parasite Plasmodium falciparum

Peter Ellekvist, Christina Høier Ricke, Thomas Litman, Ali Salanti, Hanne Colding, Thomas Zeuthen, Dan A Klaerke

23 Citations (Scopus)

Abstract

In most living cells, K(+) channels are important for the generation of the membrane potential and for volume regulation. The parasite Plasmodium falciparum, which causes malignant malaria, must be able to deal with large variations in the ambient K(+) concentration: it is exposed to high concentrations of K(+) when inside the erythrocyte and low concentrations when in plasma. In the recently published genome of P. falciparum, we have identified a gene, pfkch1, encoding a potential K(+) channel, which to some extent resembles the big-conductance (BK) K(+) channel. We have cloned the approximately 6000 nucleotide (nt) fragment from cDNA, studied the pattern of expression of pfkch1 throughout the intraerythrocytic part of the parasite's life-cyclus, and characterized the channel on the basis of similarity to other K(+) channels from pro- and eukaryotic organisms. This P. falciparum K(+) channel could be a potential drug target.

Original language	English
Journal	Biochemical and Biophysical Research Communications
Volume	318
Issue number	2
Pages (from-to)	477-84
Number of pages	7
ISSN	0006-291X
DOIs	https://doi.org/10.1016/j.bbrc.2004.04.049
Publication status	Published - 2004

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Cite this

@article{5d9618d0b54d11ddb04f000ea68e967b,

title = "Molecular cloning of a K+ channel from the malaria parasite Plasmodium falciparum",

abstract = "In most living cells, K(+) channels are important for the generation of the membrane potential and for volume regulation. The parasite Plasmodium falciparum, which causes malignant malaria, must be able to deal with large variations in the ambient K(+) concentration: it is exposed to high concentrations of K(+) when inside the erythrocyte and low concentrations when in plasma. In the recently published genome of P. falciparum, we have identified a gene, pfkch1, encoding a potential K(+) channel, which to some extent resembles the big-conductance (BK) K(+) channel. We have cloned the approximately 6000 nucleotide (nt) fragment from cDNA, studied the pattern of expression of pfkch1 throughout the intraerythrocytic part of the parasite's life-cyclus, and characterized the channel on the basis of similarity to other K(+) channels from pro- and eukaryotic organisms. This P. falciparum K(+) channel could be a potential drug target.",

author = "Peter Ellekvist and Ricke, {Christina H{\o}ier} and Thomas Litman and Ali Salanti and Hanne Colding and Thomas Zeuthen and Klaerke, {Dan A}",

note = "Keywords: Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; Conserved Sequence; Erythrocytes; Gene Expression; Humans; Malaria; Membrane Proteins; Molecular Sequence Data; Phylogeny; Plasmodium falciparum; Potassium Channels; Protozoan Proteins; RNA, Messenger; Sequence Alignment",

year = "2004",

doi = "10.1016/j.bbrc.2004.04.049",

language = "English",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Molecular cloning of a K+ channel from the malaria parasite Plasmodium falciparum

AU - Ellekvist, Peter

AU - Ricke, Christina Høier

AU - Litman, Thomas

AU - Salanti, Ali

AU - Colding, Hanne

AU - Zeuthen, Thomas

AU - Klaerke, Dan A

N1 - Keywords: Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; Conserved Sequence; Erythrocytes; Gene Expression; Humans; Malaria; Membrane Proteins; Molecular Sequence Data; Phylogeny; Plasmodium falciparum; Potassium Channels; Protozoan Proteins; RNA, Messenger; Sequence Alignment

PY - 2004

Y1 - 2004

N2 - In most living cells, K(+) channels are important for the generation of the membrane potential and for volume regulation. The parasite Plasmodium falciparum, which causes malignant malaria, must be able to deal with large variations in the ambient K(+) concentration: it is exposed to high concentrations of K(+) when inside the erythrocyte and low concentrations when in plasma. In the recently published genome of P. falciparum, we have identified a gene, pfkch1, encoding a potential K(+) channel, which to some extent resembles the big-conductance (BK) K(+) channel. We have cloned the approximately 6000 nucleotide (nt) fragment from cDNA, studied the pattern of expression of pfkch1 throughout the intraerythrocytic part of the parasite's life-cyclus, and characterized the channel on the basis of similarity to other K(+) channels from pro- and eukaryotic organisms. This P. falciparum K(+) channel could be a potential drug target.

AB - In most living cells, K(+) channels are important for the generation of the membrane potential and for volume regulation. The parasite Plasmodium falciparum, which causes malignant malaria, must be able to deal with large variations in the ambient K(+) concentration: it is exposed to high concentrations of K(+) when inside the erythrocyte and low concentrations when in plasma. In the recently published genome of P. falciparum, we have identified a gene, pfkch1, encoding a potential K(+) channel, which to some extent resembles the big-conductance (BK) K(+) channel. We have cloned the approximately 6000 nucleotide (nt) fragment from cDNA, studied the pattern of expression of pfkch1 throughout the intraerythrocytic part of the parasite's life-cyclus, and characterized the channel on the basis of similarity to other K(+) channels from pro- and eukaryotic organisms. This P. falciparum K(+) channel could be a potential drug target.

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DO - 10.1016/j.bbrc.2004.04.049

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JO - Biochemical and Biophysical Research Communications

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