Molecular characterization of the submergence response of Arabidopsis thaliana ecotype Columbia

TY - JOUR

T1 - Molecular characterization of the submergence response of Arabidopsis thaliana ecotype Columbia

AU - Lee, S.C.

AU - Mustroph, A.

AU - Sasidaharan, R.

AU - Vashisht, D.

AU - Pedersen, Ole

AU - Oosumi, T.

AU - Voesenek, L.A.C.J.

AU - Bailey-Serres, J.

PY - 2011/4

Y1 - 2011/4

N2 - A detailed description of the molecular response of Arabidopsis thaliana to submergence can aid the identification of genes that are critical to flooding survival.•Rosette-stage plants were fully submerged in complete darkness and shoot and root tissue was harvested separately after the O2 partial pressure of the petiole and root had stabilized at c. 6 and 0.1 kPa, respectively. As controls, plants were untreated or exposed to darkness. Following quantitative profiling of cellular mRNAs with the Affymetrix ATH1 platform, changes in the transcriptome in response to submergence, early darkness, and O2-deprivation were evaluated by fuzzy k-means clustering. This identified genes co-regulated at the conditional, developmental or organ-specific level. Mutants for 10 differentially expressed HYPOXIA-RESPONSIVE UNKNOWN PROTEIN (HUP) genes were screened for altered submergence tolerance.•The analysis identified 34 genes that were ubiquitously co-regulated by submergence and O2 deprivation. The biological functions of these include signaling, transcription, and anaerobic energy metabolism. HUPs comprised 40% of the co-regulated transcripts and mutants of seven of these genes were significantly altered in submergence tolerance.•The results define transcriptomic adjustments in response to submergence in the dark and demonstrate that the manipulation of HUPs can alter submergence tolerance.

AB - A detailed description of the molecular response of Arabidopsis thaliana to submergence can aid the identification of genes that are critical to flooding survival.•Rosette-stage plants were fully submerged in complete darkness and shoot and root tissue was harvested separately after the O2 partial pressure of the petiole and root had stabilized at c. 6 and 0.1 kPa, respectively. As controls, plants were untreated or exposed to darkness. Following quantitative profiling of cellular mRNAs with the Affymetrix ATH1 platform, changes in the transcriptome in response to submergence, early darkness, and O2-deprivation were evaluated by fuzzy k-means clustering. This identified genes co-regulated at the conditional, developmental or organ-specific level. Mutants for 10 differentially expressed HYPOXIA-RESPONSIVE UNKNOWN PROTEIN (HUP) genes were screened for altered submergence tolerance.•The analysis identified 34 genes that were ubiquitously co-regulated by submergence and O2 deprivation. The biological functions of these include signaling, transcription, and anaerobic energy metabolism. HUPs comprised 40% of the co-regulated transcripts and mutants of seven of these genes were significantly altered in submergence tolerance.•The results define transcriptomic adjustments in response to submergence in the dark and demonstrate that the manipulation of HUPs can alter submergence tolerance.

U2 - 10.1111/j.1469-8137.2010.03590.x

DO - 10.1111/j.1469-8137.2010.03590.x

M3 - Journal article

C2 - 21231933

SN - 0028-646X

VL - 190

SP - 457

EP - 471

JO - New Phytologist

JF - New Phytologist

IS - 2

ER -