Abstract
Yam beans (Pachyrhizus DC.) are legume root
crops that have attracted scientific interest because of high
contents of starch, protein, and iron in their roots. In this study,
58 accessions of three cultivated Pachyrhizus species were
characterized by amplified fragment length polymorphism
(AFLP) molecular markers in order to estimate genetic diversity
and interspecific relationships. To complement molecular
marker information, individuals from each accession were
analyzed in order to confirmploidy levels. Eight AFLP primer
combinations detected 136 (68.7 %) polymorphic bands.
Shannon’s diversity indices (Hs) for each species were 1.04
(P. ahipa), 1.07 (P. tuberosus), and 2.42 (P. erosus), while the
total diversity index was 2.45. Phylogenetic analysis, principal
coordinate analysis and analysis of molecular variance
(FST=0.796) all showed significant species differentiation.
All accessions were diploid (2n=2x=22), which is characteristic
of the tribe Phaseoleae. Finally, a misclassified accession
of P. tuberosus was identified. Molecular characterization of
accessions is necessary for efficient management of germplasm
collections.
crops that have attracted scientific interest because of high
contents of starch, protein, and iron in their roots. In this study,
58 accessions of three cultivated Pachyrhizus species were
characterized by amplified fragment length polymorphism
(AFLP) molecular markers in order to estimate genetic diversity
and interspecific relationships. To complement molecular
marker information, individuals from each accession were
analyzed in order to confirmploidy levels. Eight AFLP primer
combinations detected 136 (68.7 %) polymorphic bands.
Shannon’s diversity indices (Hs) for each species were 1.04
(P. ahipa), 1.07 (P. tuberosus), and 2.42 (P. erosus), while the
total diversity index was 2.45. Phylogenetic analysis, principal
coordinate analysis and analysis of molecular variance
(FST=0.796) all showed significant species differentiation.
All accessions were diploid (2n=2x=22), which is characteristic
of the tribe Phaseoleae. Finally, a misclassified accession
of P. tuberosus was identified. Molecular characterization of
accessions is necessary for efficient management of germplasm
collections.
| Original language | English |
|---|---|
| Journal | Tropical Plant Biology |
| Volume | 7 |
| Issue number | 3-4 |
| Pages (from-to) | 121-132 |
| Number of pages | 12 |
| ISSN | 1935-9756 |
| DOIs | |
| Publication status | Published - 27 Nov 2014 |
Keywords
- Faculty of Science
- Pachyrhizus . Molecular characterization . Ploidy . AFLP .American yambean . Genetic diversity