Modulatory Effect of Human Plasma on the Internal Nanostructure and Size Characteristics of Liquid-Crystalline Nanocarriers

Intan Diana Binti Mat Azmi; Linping Wu; Peter Popp Wibroe; Christa Nilsson; Jesper Østergaard; Stefan Stürup; Bente Gammelgaard; Arto Urtti; Seyed Moein Moghimi; Anan Yaghmur

doi:10.1021/acs.langmuir.5b00830

Modulatory Effect of Human Plasma on the Internal Nanostructure and Size Characteristics of Liquid-Crystalline Nanocarriers

Intan Diana Binti Mat Azmi, Linping Wu, Peter Popp Wibroe, Christa Nilsson, Jesper Østergaard, Stefan Stürup, Bente Gammelgaard, Arto Urtti, Seyed Moein Moghimi, Anan Yaghmur

40 Citations (Scopus)

Abstract

The inverted-type liquid-crystalline dispersions comprising cubosomes and hexosomes hold much potential for drug solubilization and site-specific targeting on intravenous administration. Limited information, however, is available on the influence of plasma components on nanostructural and morphological features of cubosome and hexosome dispersions, which may modulate their stability in the blood and their overall biological performance. Through an integrated approach involving SAXS, cryo-TEM, and nanoparticle tracking analysis (NTA) we have studied the time-dependent effect of human plasma (and the plasma complement system) on the integrity of the internal nanostructure, morphology, and fluctuation in size distribution of phytantriol (PHYT)-based nonlamellar crystalline dispersions. The results indicate that in the presence of plasma the internal nanostructure undergoes a transition from the biphasic phase (a bicontinuous cubic phase with symmetry Pn3m coexisting with an inverted-type hexagonal (H2) phase) to a neat hexagonal (H2) phase, which decreases the median particle size. These observations were independent of a direct effect by serum albumin and dispersion-mediated complement activation. The implication of these observations in relation to soft nanocarrier design for intravenous drug delivery is discussed.

Original language	English
Journal	Langmuir
Volume	31
Issue number	18
Pages (from-to)	5042–49
Number of pages	8
ISSN	0743-7463
DOIs	https://doi.org/10.1021/acs.langmuir.5b00830
Publication status	Published - 12 May 2015

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@article{aa70983d10b74d70971817797c2f5005,

title = "Modulatory Effect of Human Plasma on the Internal Nanostructure and Size Characteristics of Liquid-Crystalline Nanocarriers",

abstract = "The inverted-type liquid-crystalline dispersions comprising cubosomes and hexosomes hold much potential for drug solubilization and site-specific targeting on intravenous administration. Limited information, however, is available on the influence of plasma components on nanostructural and morphological features of cubosome and hexosome dispersions, which may modulate their stability in the blood and their overall biological performance. Through an integrated approach involving SAXS, cryo-TEM, and nanoparticle tracking analysis (NTA) we have studied the time-dependent effect of human plasma (and the plasma complement system) on the integrity of the internal nanostructure, morphology, and fluctuation in size distribution of phytantriol (PHYT)-based nonlamellar crystalline dispersions. The results indicate that in the presence of plasma the internal nanostructure undergoes a transition from the biphasic phase (a bicontinuous cubic phase with symmetry Pn3m coexisting with an inverted-type hexagonal (H2) phase) to a neat hexagonal (H2) phase, which decreases the median particle size. These observations were independent of a direct effect by serum albumin and dispersion-mediated complement activation. The implication of these observations in relation to soft nanocarrier design for intravenous drug delivery is discussed.",

author = "{Mat Azmi}, {Intan Diana Binti} and Linping Wu and Wibroe, {Peter Popp} and Christa Nilsson and Jesper {\O}stergaard and Stefan St{\"u}rup and Bente Gammelgaard and Arto Urtti and Moghimi, {Seyed Moein} and Anan Yaghmur",

year = "2015",

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doi = "10.1021/acs.langmuir.5b00830",

language = "English",

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journal = "Langmuir",

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publisher = "American Chemical Society",

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TY - JOUR

T1 - Modulatory Effect of Human Plasma on the Internal Nanostructure and Size Characteristics of Liquid-Crystalline Nanocarriers

AU - Mat Azmi, Intan Diana Binti

AU - Wu, Linping

AU - Wibroe, Peter Popp

AU - Nilsson, Christa

AU - Østergaard, Jesper

AU - Stürup, Stefan

AU - Gammelgaard, Bente

AU - Urtti, Arto

AU - Moghimi, Seyed Moein

AU - Yaghmur, Anan

PY - 2015/5/12

Y1 - 2015/5/12

N2 - The inverted-type liquid-crystalline dispersions comprising cubosomes and hexosomes hold much potential for drug solubilization and site-specific targeting on intravenous administration. Limited information, however, is available on the influence of plasma components on nanostructural and morphological features of cubosome and hexosome dispersions, which may modulate their stability in the blood and their overall biological performance. Through an integrated approach involving SAXS, cryo-TEM, and nanoparticle tracking analysis (NTA) we have studied the time-dependent effect of human plasma (and the plasma complement system) on the integrity of the internal nanostructure, morphology, and fluctuation in size distribution of phytantriol (PHYT)-based nonlamellar crystalline dispersions. The results indicate that in the presence of plasma the internal nanostructure undergoes a transition from the biphasic phase (a bicontinuous cubic phase with symmetry Pn3m coexisting with an inverted-type hexagonal (H2) phase) to a neat hexagonal (H2) phase, which decreases the median particle size. These observations were independent of a direct effect by serum albumin and dispersion-mediated complement activation. The implication of these observations in relation to soft nanocarrier design for intravenous drug delivery is discussed.

AB - The inverted-type liquid-crystalline dispersions comprising cubosomes and hexosomes hold much potential for drug solubilization and site-specific targeting on intravenous administration. Limited information, however, is available on the influence of plasma components on nanostructural and morphological features of cubosome and hexosome dispersions, which may modulate their stability in the blood and their overall biological performance. Through an integrated approach involving SAXS, cryo-TEM, and nanoparticle tracking analysis (NTA) we have studied the time-dependent effect of human plasma (and the plasma complement system) on the integrity of the internal nanostructure, morphology, and fluctuation in size distribution of phytantriol (PHYT)-based nonlamellar crystalline dispersions. The results indicate that in the presence of plasma the internal nanostructure undergoes a transition from the biphasic phase (a bicontinuous cubic phase with symmetry Pn3m coexisting with an inverted-type hexagonal (H2) phase) to a neat hexagonal (H2) phase, which decreases the median particle size. These observations were independent of a direct effect by serum albumin and dispersion-mediated complement activation. The implication of these observations in relation to soft nanocarrier design for intravenous drug delivery is discussed.

U2 - 10.1021/acs.langmuir.5b00830

DO - 10.1021/acs.langmuir.5b00830

M3 - Journal article

C2 - 25884233

SN - 0743-7463

VL - 31

SP - 5042

EP - 5049

JO - Langmuir

JF - Langmuir

IS - 18

ER -

Modulatory Effect of Human Plasma on the Internal Nanostructure and Size Characteristics of Liquid-Crystalline Nanocarriers

Abstract

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