@inbook{8f65b405d6194ea8a0d010c0dc137922,
title = "Modulating the levels of plant hormone cytokinins at the host-pathogen interface",
abstract = "Cytokinins are adenine and non-adenine derived heterogeneous class of regulatory molecules that participate in almost every aspect of plant biology. They also affect plant defense responses as well as help microbial pathogens to establish pathogenesis. The functional approaches that ensure desired and subtle modulations in the levels of plant cytokinins are highly instrumental in assessing their functions in plant immunity. Here, we describe a detailed working protocol regarding the enhanced production of cytokinins from plants that harbor isopentenyltransferase (IPT) enzyme gene under the control of 4xJERE (jasmonic acid and elicitor-responsive element) pathogen-inducible promoter. Our devised expression system is a context-dependent solution when it comes to investigating host-pathogen interactions under the modulated conditions of plant cytokinins.",
keywords = "Journal Article",
author = "Muhammad Naseem and Shabana Shams and Roitsch, {Thomas Georg}",
year = "2017",
doi = "10.1007/978-1-4939-6831-2_11",
language = "English",
isbn = "978-1-4939-6829-9",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "141--150",
editor = "Thomas Dandekar and Muhammad Naseem",
booktitle = "Auxins and cytokinins in plant biology",
}