Modified PAMAM dendrimer with 4-carbomethoxypyrrolidone surface groups reveals negligible toxicity against three rodent cell-lines

Anna Janaszewska; Michal Ciolkowski; Dominika Wróbel; Johannes Fabritius Petersen; Mario Ficker; Jørn Bolstad Christensen; Maria Bryszewska; Barbara Klajnert

doi:10.1016/j.nano.2013.01.010

Modified PAMAM dendrimer with 4-carbomethoxypyrrolidone surface groups reveals negligible toxicity against three rodent cell-lines

Anna Janaszewska, Michal Ciolkowski, Dominika Wróbel, Johannes Fabritius Petersen, Mario Ficker, Jørn Bolstad Christensen, Maria Bryszewska, Barbara Klajnert

Department of Chemistry

40 Citations (Scopus)

Abstract

Modification of the surface groups of dendrimers is one of the methods to improve their biocompatibility. This article presents results of experiments related to the toxicity of a modified polyamidoamine (PAMAM) dendrimer of the fourth generation with 4-carbomethoxypyrrolidone surface groups (PAMAM-pyrrolidone dendrimer). The cytotoxic activity of the dendrimer was tested on Chinese hamster fibroblasts (B14), embryonic mouse hippocampal cells (mHippoE-18) and rat liver derived cells (BRL-3A). The same cell lines were used to investigate the influence of pyrrolidone dendrimer on the mitochondrial membrane potential, intracellular ROS level and its ability to induce apoptosis or necrosis. The analyzed dendrimer showed only minor toxicity and no ability to induce apoptosis. The most important finding is the lack of influence of the PAMAM-pyrrolidone dendrimer on intracellular ROS level and mitochondrial membrane potential.

Original language	English
Journal	Nanomedicine: Nanotechnology, Biology and Medicine
Volume	9
Issue number	4
Pages (from-to)	461-464
Number of pages	4
ISSN	1549-9634
DOIs	https://doi.org/10.1016/j.nano.2013.01.010
Publication status	Published - May 2013

Keywords

Animals
Cell Line
Cricetinae
Cricetulus
Dendrimers
Mice
Pyrrolidinones
Rats

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10.1016/j.nano.2013.01.010

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Janaszewska, A., Ciolkowski, M., Wróbel, D., Petersen, J. F., Ficker, M., Christensen, J. B., Bryszewska, M., & Klajnert, B. (2013). Modified PAMAM dendrimer with 4-carbomethoxypyrrolidone surface groups reveals negligible toxicity against three rodent cell-lines. Nanomedicine: Nanotechnology, Biology and Medicine, 9(4), 461-464. https://doi.org/10.1016/j.nano.2013.01.010

Modified PAMAM dendrimer with 4-carbomethoxypyrrolidone surface groups reveals negligible toxicity against three rodent cell-lines. / Janaszewska, Anna; Ciolkowski, Michal; Wróbel, Dominika et al.

In: Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 9, No. 4, 05.2013, p. 461-464.

Research output: Contribution to journal › Journal article › Research › peer-review

Janaszewska, A, Ciolkowski, M, Wróbel, D, Petersen, JF, Ficker, M, Christensen, JB, Bryszewska, M & Klajnert, B 2013, 'Modified PAMAM dendrimer with 4-carbomethoxypyrrolidone surface groups reveals negligible toxicity against three rodent cell-lines', Nanomedicine: Nanotechnology, Biology and Medicine, vol. 9, no. 4, pp. 461-464. https://doi.org/10.1016/j.nano.2013.01.010

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title = "Modified PAMAM dendrimer with 4-carbomethoxypyrrolidone surface groups reveals negligible toxicity against three rodent cell-lines",

abstract = "Modification of the surface groups of dendrimers is one of the methods to improve their biocompatibility. This article presents results of experiments related to the toxicity of a modified polyamidoamine (PAMAM) dendrimer of the fourth generation with 4-carbomethoxypyrrolidone surface groups (PAMAM-pyrrolidone dendrimer). The cytotoxic activity of the dendrimer was tested on Chinese hamster fibroblasts (B14), embryonic mouse hippocampal cells (mHippoE-18) and rat liver derived cells (BRL-3A). The same cell lines were used to investigate the influence of pyrrolidone dendrimer on the mitochondrial membrane potential, intracellular ROS level and its ability to induce apoptosis or necrosis. The analyzed dendrimer showed only minor toxicity and no ability to induce apoptosis. The most important finding is the lack of influence of the PAMAM-pyrrolidone dendrimer on intracellular ROS level and mitochondrial membrane potential.",

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author = "Anna Janaszewska and Michal Ciolkowski and Dominika Wr{\'o}bel and Petersen, {Johannes Fabritius} and Mario Ficker and Christensen, {J{\o}rn Bolstad} and Maria Bryszewska and Barbara Klajnert",

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AU - Petersen, Johannes Fabritius

AU - Ficker, Mario

AU - Christensen, Jørn Bolstad

AU - Bryszewska, Maria

AU - Klajnert, Barbara

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N2 - Modification of the surface groups of dendrimers is one of the methods to improve their biocompatibility. This article presents results of experiments related to the toxicity of a modified polyamidoamine (PAMAM) dendrimer of the fourth generation with 4-carbomethoxypyrrolidone surface groups (PAMAM-pyrrolidone dendrimer). The cytotoxic activity of the dendrimer was tested on Chinese hamster fibroblasts (B14), embryonic mouse hippocampal cells (mHippoE-18) and rat liver derived cells (BRL-3A). The same cell lines were used to investigate the influence of pyrrolidone dendrimer on the mitochondrial membrane potential, intracellular ROS level and its ability to induce apoptosis or necrosis. The analyzed dendrimer showed only minor toxicity and no ability to induce apoptosis. The most important finding is the lack of influence of the PAMAM-pyrrolidone dendrimer on intracellular ROS level and mitochondrial membrane potential.

AB - Modification of the surface groups of dendrimers is one of the methods to improve their biocompatibility. This article presents results of experiments related to the toxicity of a modified polyamidoamine (PAMAM) dendrimer of the fourth generation with 4-carbomethoxypyrrolidone surface groups (PAMAM-pyrrolidone dendrimer). The cytotoxic activity of the dendrimer was tested on Chinese hamster fibroblasts (B14), embryonic mouse hippocampal cells (mHippoE-18) and rat liver derived cells (BRL-3A). The same cell lines were used to investigate the influence of pyrrolidone dendrimer on the mitochondrial membrane potential, intracellular ROS level and its ability to induce apoptosis or necrosis. The analyzed dendrimer showed only minor toxicity and no ability to induce apoptosis. The most important finding is the lack of influence of the PAMAM-pyrrolidone dendrimer on intracellular ROS level and mitochondrial membrane potential.

KW - Animals

KW - Cell Line

KW - Cricetinae

KW - Cricetulus

KW - Dendrimers

KW - Mice

KW - Pyrrolidinones

KW - Rats

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JF - Nanomedicine: Nanotechnology, Biology and Medicine

IS - 4

ER -

Modified PAMAM dendrimer with 4-carbomethoxypyrrolidone surface groups reveals negligible toxicity against three rodent cell-lines

Abstract

Keywords

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