Mitovirus and Mitochondrial Coding Sequences from Basal Fungus Entomophthora muscae

TY - JOUR

T1 - Mitovirus and Mitochondrial Coding Sequences from Basal Fungus Entomophthora muscae

AU - Nibert, Max L.

AU - Debat, Humberto J.

AU - Manny, Austin R.

AU - Grigoriev, Igor V.

AU - De Fine Licht, Henrik H.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Fungi constituting the Entomophthora muscae species complex (members of subphylum Entomophthoromycotina, phylum Zoopagamycota) commonly kill their insect hosts and manipulate host behaviors in the process. In this study, we made use of public transcriptome data to identify and characterize eight new species of mitoviruses associated with several different E. muscae isolates. Mitoviruses are simple RNA viruses that replicate in host mitochondria and are frequently found in more phylogenetically apical fungi (members of subphylum Glomeromyoctina, phylum Mucoromycota, phylum Basidiomycota and phylum Ascomycota) as well as in plants. E. muscae is the first fungus from phylum Zoopagomycota, and thereby the most phylogenetically basal fungus, found to harbor mitoviruses to date. Multiple UGA (Trp) codons are found not only in each of the new mitovirus sequences from E. muscae but also in mitochondrial core-gene coding sequences newly assembled from E. muscae transcriptome data, suggesting that UGA (Trp) is not a rarely used codon in the mitochondria of this fungus. The presence of mitoviruses in these basal fungi has possible implications for the evolution of these viruses.

AB - Fungi constituting the Entomophthora muscae species complex (members of subphylum Entomophthoromycotina, phylum Zoopagamycota) commonly kill their insect hosts and manipulate host behaviors in the process. In this study, we made use of public transcriptome data to identify and characterize eight new species of mitoviruses associated with several different E. muscae isolates. Mitoviruses are simple RNA viruses that replicate in host mitochondria and are frequently found in more phylogenetically apical fungi (members of subphylum Glomeromyoctina, phylum Mucoromycota, phylum Basidiomycota and phylum Ascomycota) as well as in plants. E. muscae is the first fungus from phylum Zoopagomycota, and thereby the most phylogenetically basal fungus, found to harbor mitoviruses to date. Multiple UGA (Trp) codons are found not only in each of the new mitovirus sequences from E. muscae but also in mitochondrial core-gene coding sequences newly assembled from E. muscae transcriptome data, suggesting that UGA (Trp) is not a rarely used codon in the mitochondria of this fungus. The presence of mitoviruses in these basal fungi has possible implications for the evolution of these viruses.

KW - Database mining

KW - Entomophthora

KW - Entomophthoromycotina

KW - Fungal virus

KW - Mitochondrion

KW - Mitovirus

KW - Mycovirus

KW - Narnaviridae

KW - Virus discovery

UR - http://www.scopus.com/inward/record.url?scp=85065047072&partnerID=8YFLogxK

U2 - 10.3390/v11040351

DO - 10.3390/v11040351

M3 - Journal article

C2 - 30999558

AN - SCOPUS:85065047072

SN - 1999-4915

VL - 11

SP - 1

EP - 17

JO - Viruses

JF - Viruses

IS - 4

M1 - 351

ER -