miR-200b mediates post-transcriptional repression of ZFHX1B.

Nanna Rønbjerg Christoffersen; Asli Silahtaroglu; Ulf Lupo Andersson Ørom; Markus Sakari Kauppinen; Anders H Lund

doi:10.1261/rna.586807

miR-200b mediates post-transcriptional repression of ZFHX1B.

Nanna Rønbjerg Christoffersen, Asli Silahtaroglu, Ulf Lupo Andersson Ørom, Markus Sakari Kauppinen, Anders H Lund

Medical Genetics Program

143 Citations (Scopus)

Abstract

MicroRNAs have important functions during animal development and homeostasis through post-transcriptional regulation of their cognate mRNA targets. ZFHX1B is a transcriptional repressor involved in the TGFbeta signaling pathway and in processes of epithelial to mesenchymal transition via regulation of E-cadherin. We show that Zfhx1b and miR-200b are regionally coexpressed in the adult mouse brain and that miR-200b represses the expression of Zfhx1b via multiple sequence elements present in the 3'-untranslated region. Overexpression of miR-200b leads to repression of endogenous ZFHX1B, and inhibition of miR-200b relieves the repression of ZFHX1B. In accordance with these findings, miR-200b regulates the activity of the E-cadherin promoter.

Original language	English
Journal	RNA: A publication of the RNA Society
Volume	13
Issue number	8
Pages (from-to)	1172-8
Number of pages	6
ISSN	1355-8382
DOIs	https://doi.org/10.1261/rna.586807
Publication status	Published - 2007

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title = "miR-200b mediates post-transcriptional repression of ZFHX1B.",

abstract = "MicroRNAs have important functions during animal development and homeostasis through post-transcriptional regulation of their cognate mRNA targets. ZFHX1B is a transcriptional repressor involved in the TGFbeta signaling pathway and in processes of epithelial to mesenchymal transition via regulation of E-cadherin. We show that Zfhx1b and miR-200b are regionally coexpressed in the adult mouse brain and that miR-200b represses the expression of Zfhx1b via multiple sequence elements present in the 3'-untranslated region. Overexpression of miR-200b leads to repression of endogenous ZFHX1B, and inhibition of miR-200b relieves the repression of ZFHX1B. In accordance with these findings, miR-200b regulates the activity of the E-cadherin promoter.",

author = "Christoffersen, {Nanna R{\o}nbjerg} and Asli Silahtaroglu and {\O}rom, {Ulf Lupo Andersson} and Kauppinen, {Markus Sakari} and Lund, {Anders H}",

note = "Keywords: 3' Untranslated Regions; Animals; Cadherins; Cell Line; Homeodomain Proteins; Mice; MicroRNAs; Promoter Regions (Genetics); RNA Interference; Repressor Proteins",

year = "2007",

doi = "10.1261/rna.586807",

language = "English",

volume = "13",

pages = "1172--8",

journal = "RNA: A publication of the RNA Society",

issn = "1355-8382",

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T1 - miR-200b mediates post-transcriptional repression of ZFHX1B.

AU - Christoffersen, Nanna Rønbjerg

AU - Silahtaroglu, Asli

AU - Ørom, Ulf Lupo Andersson

AU - Kauppinen, Markus Sakari

AU - Lund, Anders H

N1 - Keywords: 3' Untranslated Regions; Animals; Cadherins; Cell Line; Homeodomain Proteins; Mice; MicroRNAs; Promoter Regions (Genetics); RNA Interference; Repressor Proteins

PY - 2007

Y1 - 2007

N2 - MicroRNAs have important functions during animal development and homeostasis through post-transcriptional regulation of their cognate mRNA targets. ZFHX1B is a transcriptional repressor involved in the TGFbeta signaling pathway and in processes of epithelial to mesenchymal transition via regulation of E-cadherin. We show that Zfhx1b and miR-200b are regionally coexpressed in the adult mouse brain and that miR-200b represses the expression of Zfhx1b via multiple sequence elements present in the 3'-untranslated region. Overexpression of miR-200b leads to repression of endogenous ZFHX1B, and inhibition of miR-200b relieves the repression of ZFHX1B. In accordance with these findings, miR-200b regulates the activity of the E-cadherin promoter.

AB - MicroRNAs have important functions during animal development and homeostasis through post-transcriptional regulation of their cognate mRNA targets. ZFHX1B is a transcriptional repressor involved in the TGFbeta signaling pathway and in processes of epithelial to mesenchymal transition via regulation of E-cadherin. We show that Zfhx1b and miR-200b are regionally coexpressed in the adult mouse brain and that miR-200b represses the expression of Zfhx1b via multiple sequence elements present in the 3'-untranslated region. Overexpression of miR-200b leads to repression of endogenous ZFHX1B, and inhibition of miR-200b relieves the repression of ZFHX1B. In accordance with these findings, miR-200b regulates the activity of the E-cadherin promoter.

U2 - 10.1261/rna.586807

DO - 10.1261/rna.586807

M3 - Journal article

C2 - 17585049

SN - 1355-8382

VL - 13

SP - 1172

EP - 1178

JO - RNA: A publication of the RNA Society

JF - RNA: A publication of the RNA Society

IS - 8

ER -

miR-200b mediates post-transcriptional repression of ZFHX1B.

Abstract

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