TY - JOUR
T1 - MicroRNA expression in melanocytic nevi: the usefulness of formalin-fixed, paraffin-embedded material for miRNA microarray profiling
AU - Glud, M.
AU - Klausen, M.
AU - Gniadecki, R.
AU - Rossing, M.
AU - Hastrup, N.
AU - Nielsen, F.C.
AU - Drzewiecki, K.T.
AU - Glud, Martin
AU - Klausen, Mikkel
AU - Gniadecki, Robert
AU - Rossing, Maria
AU - Hastrup, Nina
AU - Nielsen, Finn C
AU - Drzewiecki, Krzysztof T
N1 - Times Cited: 4ArticleEnglishGlud, MUniv Copenhagen, Dept Plast Surg, Fac Hlth Sci, Rigshosp, Blegdamsvej 9, DK-2100 Copenhagen, DenmarkCited References Count: 17447USNATURE PUBLISHING GROUP75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USANEW YORK
PY - 2009/5/1
Y1 - 2009/5/1
N2 - MicroRNAs (miRNAs) are small, noncoding RNA molecules that regulate cellular differentiation, proliferation, and apoptosis. MiRNAs are expressed in a developmentally regulated and tissue-specific manner. Aberrant expression may contribute to pathological processes such as cancer, and miRNA may therefore serve as biomarkers that may be useful in a clinical environment for diagnosis of various diseases. Most miRNA profiling studies have used fresh tissue samples. However, in some types of cancer, including malignant melanoma, fresh material is difficult to obtain from primary tumors, and most surgical specimens are formalin fixed and paraffin embedded (FFPE). To explore whether FFPE material would be suitable for miRNA profiling in melanocytic lesions, we compared miRNA expression patterns in FFPE versus fresh frozen samples, obtained from 15 human melanocytic nevi. Out of microarray data, we identified 84 miRNAs that were expressed in both types of samples and represented an miRNA profile of melanocytic nevi. Our results showed a high correlation in miRNA expression (Spearman r-value of 0.80) between paired FFPE and fresh frozen material. The data were further validated by quantitative RT-PCR. In conclusion, FFPE specimens of melanocytic lesions are suitable as a source for miRNA microarray profiling.
AB - MicroRNAs (miRNAs) are small, noncoding RNA molecules that regulate cellular differentiation, proliferation, and apoptosis. MiRNAs are expressed in a developmentally regulated and tissue-specific manner. Aberrant expression may contribute to pathological processes such as cancer, and miRNA may therefore serve as biomarkers that may be useful in a clinical environment for diagnosis of various diseases. Most miRNA profiling studies have used fresh tissue samples. However, in some types of cancer, including malignant melanoma, fresh material is difficult to obtain from primary tumors, and most surgical specimens are formalin fixed and paraffin embedded (FFPE). To explore whether FFPE material would be suitable for miRNA profiling in melanocytic lesions, we compared miRNA expression patterns in FFPE versus fresh frozen samples, obtained from 15 human melanocytic nevi. Out of microarray data, we identified 84 miRNAs that were expressed in both types of samples and represented an miRNA profile of melanocytic nevi. Our results showed a high correlation in miRNA expression (Spearman r-value of 0.80) between paired FFPE and fresh frozen material. The data were further validated by quantitative RT-PCR. In conclusion, FFPE specimens of melanocytic lesions are suitable as a source for miRNA microarray profiling.
U2 - 10.1038/jid.2008.347
DO - 10.1038/jid.2008.347
M3 - Journal article
SN - 0022-202X
VL - 129
SP - 1219
EP - 1224
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 5
ER -
