Microfluidic bioreactors for culture of non-adherent cells

Pranjul Jaykumar Shah; Indumathi Vedarethinam; Dorota Kwasny; Lars Andresen; Maria Dimaki; Søren Skov; Winnie Edith Svendsen

doi:10.1016/j.snb.2011.02.021

Microfluidic bioreactors for culture of non-adherent cells

Pranjul Jaykumar Shah, Indumathi Vedarethinam, Dorota Kwasny, Lars Andresen, Maria Dimaki, Søren Skov, Winnie Edith Svendsen

21 Citations (Scopus)

Abstract

Microfluidic bioreactors (μBR) are becoming increasingly popular for cell culture, sample preparation and analysis in case of routine genetic and clinical diagnostics. We present a novel μBR for non-adherent cells designed to mimic in vivo perfusion of cells based on diffusion of media through a sandwiched membrane. The culture chamber and perfusion chamber are separated by a sandwiched membrane and each chamber has separate inlet/outlets for easy loading/unloading of cells and perfusion of the media. The perfusion of media and exchange of nutrients occur through the sandwiched membrane, which was also verified with simulations. Finally, we present the application of this device for cytogenetic sample preparation, whereby we culture and arrest peripheral T-lymphocytes in metaphase and later fix them in the μBR. The expansion of T-lymphocytes from an unknown patient sample was quantified by means of CFSE staining and subsequent counting in a flow cytometer. To conclude on the applicability of μBR for genetic diagnostics, we prepare chromosome spreads on glass slides from the cultured samples, which is the primary step for metaphase FISH analysis.

Original language	English
Journal	Sensors and Actuators B: Chemical
Volume	156
Issue number	2
Pages (from-to)	1002-1008
Number of pages	7
ISSN	0925-4005
DOIs	https://doi.org/10.1016/j.snb.2011.02.021
Publication status	Published - Aug 2011

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title = "Microfluidic bioreactors for culture of non-adherent cells",

abstract = "Microfluidic bioreactors (μBR) are becoming increasingly popular for cell culture, sample preparation and analysis in case of routine genetic and clinical diagnostics. We present a novel μBR for non-adherent cells designed to mimic in vivo perfusion of cells based on diffusion of media through a sandwiched membrane. The culture chamber and perfusion chamber are separated by a sandwiched membrane and each chamber has separate inlet/outlets for easy loading/unloading of cells and perfusion of the media. The perfusion of media and exchange of nutrients occur through the sandwiched membrane, which was also verified with simulations. Finally, we present the application of this device for cytogenetic sample preparation, whereby we culture and arrest peripheral T-lymphocytes in metaphase and later fix them in the μBR. The expansion of T-lymphocytes from an unknown patient sample was quantified by means of CFSE staining and subsequent counting in a flow cytometer. To conclude on the applicability of μBR for genetic diagnostics, we prepare chromosome spreads on glass slides from the cultured samples, which is the primary step for metaphase FISH analysis.",

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AU - Shah, Pranjul Jaykumar

AU - Vedarethinam, Indumathi

AU - Kwasny, Dorota

AU - Andresen, Lars

AU - Dimaki, Maria

AU - Skov, Søren

AU - Svendsen, Winnie Edith

PY - 2011/8

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Microfluidic bioreactors for culture of non-adherent cells

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