Microemulsions as Potential Carriers of Nisin: Effect of Composition on Structure and Efficacy

Maria D Chatzidaki; Konstantinos Papadimitriou; Voula Alexandraki; Eirini Tsirvouli; Zena Chakim; Aghiad Ghazal; Kell Mortensen; Anan Yaghmur; Stefan Salentinig; Vassiliki Papadimitriou; Effie Tsakalidou; Aristotelis Xenakis

doi:10.1021/acs.langmuir.6b02923

Microemulsions as Potential Carriers of Nisin: Effect of Composition on Structure and Efficacy

Maria D Chatzidaki, Konstantinos Papadimitriou, Voula Alexandraki, Eirini Tsirvouli, Zena Chakim, Aghiad Ghazal, Kell Mortensen, Anan Yaghmur, Stefan Salentinig, Vassiliki Papadimitriou, Effie Tsakalidou, Aristotelis Xenakis

Xray and Neutron Science

12 Citations (Scopus)

Abstract

Water-in-oil (W/O) microemulsions based on either refined olive oil (ROO) or sunflower oil (SO), distilled monoglycerides (DMG), and ethanol were used as nisin carriers in order to ensure its effectiveness as a biopreservative. This work presents experimental evidence on the effects of ethanol concentration, hydration, the nature of oil, and the addition of nisin on the nanostructure of the proposed inverse microemulsions as revealed by electrical conductivity measurements, dynamic light scattering (DLS), small angle X-ray scattering (SAXS), and electron paramagnetic resonance (EPR) spectroscopy. Modeling of representative SAXS profiles was applied to gain further insight into the effects of ethanol and solubilized water content on the inverse swollen micelles' size and morphology. With increasing ethanol content, the overall size of the inverse micelles decreased, whereas hydration resulted in an increase in the micellar size due to the penetration of water into the hydrophilic core of the inverse swollen micelles (hydration-induced swelling behavior). The dynamic properties of the surfactant monolayer were also affected by the nature of the used vegetable oil, the ethanol content, and the presence of the bioactive molecule, as evidenced by EPR spin probing experiments. According to simulation on the experimental spectra, two populations of spin probes at different polarities were revealed. The antimicrobial effect of the encapsulated nisin was evaluated using the well diffusion assay (WDA) technique against Lactococccus lactis. It was found that this encapsulated bacteriocin induced an inhibition of the microorganism growth. The effect was more pronounced at higher ethanol concentrations, but no significant difference was observed between the two used vegetable oils (ROO and SO).

Original language	English
Journal	Langmuir
Volume	32
Issue number	35
Pages (from-to)	8988-8998
ISSN	0743-7463
DOIs	https://doi.org/10.1021/acs.langmuir.6b02923
Publication status	Published - 6 Sept 2016

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Journal Article

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title = "Microemulsions as Potential Carriers of Nisin: Effect of Composition on Structure and Efficacy",

abstract = "Water-in-oil (W/O) microemulsions based on either refined olive oil (ROO) or sunflower oil (SO), distilled monoglycerides (DMG), and ethanol were used as nisin carriers in order to ensure its effectiveness as a biopreservative. This work presents experimental evidence on the effects of ethanol concentration, hydration, the nature of oil, and the addition of nisin on the nanostructure of the proposed inverse microemulsions as revealed by electrical conductivity measurements, dynamic light scattering (DLS), small angle X-ray scattering (SAXS), and electron paramagnetic resonance (EPR) spectroscopy. Modeling of representative SAXS profiles was applied to gain further insight into the effects of ethanol and solubilized water content on the inverse swollen micelles' size and morphology. With increasing ethanol content, the overall size of the inverse micelles decreased, whereas hydration resulted in an increase in the micellar size due to the penetration of water into the hydrophilic core of the inverse swollen micelles (hydration-induced swelling behavior). The dynamic properties of the surfactant monolayer were also affected by the nature of the used vegetable oil, the ethanol content, and the presence of the bioactive molecule, as evidenced by EPR spin probing experiments. According to simulation on the experimental spectra, two populations of spin probes at different polarities were revealed. The antimicrobial effect of the encapsulated nisin was evaluated using the well diffusion assay (WDA) technique against Lactococccus lactis. It was found that this encapsulated bacteriocin induced an inhibition of the microorganism growth. The effect was more pronounced at higher ethanol concentrations, but no significant difference was observed between the two used vegetable oils (ROO and SO).",

keywords = "Journal Article",

author = "Chatzidaki, {Maria D} and Konstantinos Papadimitriou and Voula Alexandraki and Eirini Tsirvouli and Zena Chakim and Aghiad Ghazal and Kell Mortensen and Anan Yaghmur and Stefan Salentinig and Vassiliki Papadimitriou and Effie Tsakalidou and Aristotelis Xenakis",

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T1 - Microemulsions as Potential Carriers of Nisin

T2 - Effect of Composition on Structure and Efficacy

AU - Chatzidaki, Maria D

AU - Papadimitriou, Konstantinos

AU - Alexandraki, Voula

AU - Tsirvouli, Eirini

AU - Chakim, Zena

AU - Ghazal, Aghiad

AU - Mortensen, Kell

AU - Yaghmur, Anan

AU - Salentinig, Stefan

AU - Papadimitriou, Vassiliki

AU - Tsakalidou, Effie

AU - Xenakis, Aristotelis

PY - 2016/9/6

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N2 - Water-in-oil (W/O) microemulsions based on either refined olive oil (ROO) or sunflower oil (SO), distilled monoglycerides (DMG), and ethanol were used as nisin carriers in order to ensure its effectiveness as a biopreservative. This work presents experimental evidence on the effects of ethanol concentration, hydration, the nature of oil, and the addition of nisin on the nanostructure of the proposed inverse microemulsions as revealed by electrical conductivity measurements, dynamic light scattering (DLS), small angle X-ray scattering (SAXS), and electron paramagnetic resonance (EPR) spectroscopy. Modeling of representative SAXS profiles was applied to gain further insight into the effects of ethanol and solubilized water content on the inverse swollen micelles' size and morphology. With increasing ethanol content, the overall size of the inverse micelles decreased, whereas hydration resulted in an increase in the micellar size due to the penetration of water into the hydrophilic core of the inverse swollen micelles (hydration-induced swelling behavior). The dynamic properties of the surfactant monolayer were also affected by the nature of the used vegetable oil, the ethanol content, and the presence of the bioactive molecule, as evidenced by EPR spin probing experiments. According to simulation on the experimental spectra, two populations of spin probes at different polarities were revealed. The antimicrobial effect of the encapsulated nisin was evaluated using the well diffusion assay (WDA) technique against Lactococccus lactis. It was found that this encapsulated bacteriocin induced an inhibition of the microorganism growth. The effect was more pronounced at higher ethanol concentrations, but no significant difference was observed between the two used vegetable oils (ROO and SO).

AB - Water-in-oil (W/O) microemulsions based on either refined olive oil (ROO) or sunflower oil (SO), distilled monoglycerides (DMG), and ethanol were used as nisin carriers in order to ensure its effectiveness as a biopreservative. This work presents experimental evidence on the effects of ethanol concentration, hydration, the nature of oil, and the addition of nisin on the nanostructure of the proposed inverse microemulsions as revealed by electrical conductivity measurements, dynamic light scattering (DLS), small angle X-ray scattering (SAXS), and electron paramagnetic resonance (EPR) spectroscopy. Modeling of representative SAXS profiles was applied to gain further insight into the effects of ethanol and solubilized water content on the inverse swollen micelles' size and morphology. With increasing ethanol content, the overall size of the inverse micelles decreased, whereas hydration resulted in an increase in the micellar size due to the penetration of water into the hydrophilic core of the inverse swollen micelles (hydration-induced swelling behavior). The dynamic properties of the surfactant monolayer were also affected by the nature of the used vegetable oil, the ethanol content, and the presence of the bioactive molecule, as evidenced by EPR spin probing experiments. According to simulation on the experimental spectra, two populations of spin probes at different polarities were revealed. The antimicrobial effect of the encapsulated nisin was evaluated using the well diffusion assay (WDA) technique against Lactococccus lactis. It was found that this encapsulated bacteriocin induced an inhibition of the microorganism growth. The effect was more pronounced at higher ethanol concentrations, but no significant difference was observed between the two used vegetable oils (ROO and SO).

KW - Journal Article

U2 - 10.1021/acs.langmuir.6b02923

DO - 10.1021/acs.langmuir.6b02923

M3 - Journal article

C2 - 27501392

SN - 0743-7463

VL - 32

SP - 8988

EP - 8998

JO - Langmuir

JF - Langmuir

IS - 35

ER -

Microemulsions as Potential Carriers of Nisin: Effect of Composition on Structure and Efficacy

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