TY - JOUR
T1 - Microbial production of indolylglucosinolate through engineering of a multi-gene pathway in a versatile yeast expression platform
AU - Mikkelsen, Michael Dalgaard
AU - Buron, Line Due
AU - Salomonsen, Bo
AU - Olsen, Carl Erik
AU - Hansen, Bjarne Gram
AU - Mortensen, Uffe Hasbro
AU - Halkier, Barbara Ann
PY - 2012/3
Y1 - 2012/3
N2 - Epidemiological studies have shown that consumption of cruciferous vegetables, such as, broccoli and cabbages, is associated with a reduced risk of developing cancer. This phenomenon has been attributed to specific glucosinolates among the ~30 glucosinolates that are typically present as natural products characteristic of cruciferous plants. Accordingly, there has been a strong interest to produce these compounds in microbial cell factories as it will allow production of selected beneficial glucosinolates. We have developed a versatile platform for stable expression of multi-gene pathways in the yeast, Saccharomyces cerevisiae. Introduction of the seven-step pathway of indolylglucosinolate from Arabidopsis thaliana to yeast resulted in the first successful production of glucosinolates in a microbial host. The production of indolylglucosinolate was further optimized by substituting supporting endogenous yeast activities with plant-derived enzymes. Production of indolylglucosinolate serves as a proof-of-concept for our expression platform, and provides a basis for large-scale microbial production of specific glucosinolates for the benefit of human health.
AB - Epidemiological studies have shown that consumption of cruciferous vegetables, such as, broccoli and cabbages, is associated with a reduced risk of developing cancer. This phenomenon has been attributed to specific glucosinolates among the ~30 glucosinolates that are typically present as natural products characteristic of cruciferous plants. Accordingly, there has been a strong interest to produce these compounds in microbial cell factories as it will allow production of selected beneficial glucosinolates. We have developed a versatile platform for stable expression of multi-gene pathways in the yeast, Saccharomyces cerevisiae. Introduction of the seven-step pathway of indolylglucosinolate from Arabidopsis thaliana to yeast resulted in the first successful production of glucosinolates in a microbial host. The production of indolylglucosinolate was further optimized by substituting supporting endogenous yeast activities with plant-derived enzymes. Production of indolylglucosinolate serves as a proof-of-concept for our expression platform, and provides a basis for large-scale microbial production of specific glucosinolates for the benefit of human health.
U2 - 10.1016/j.ymben.2012.01.006
DO - 10.1016/j.ymben.2012.01.006
M3 - Journal article
C2 - 22326477
SN - 1096-7176
VL - 14
SP - 104
EP - 111
JO - Metabolic Engineering
JF - Metabolic Engineering
IS - 2
ER -
