Methods to classify bacterial pathogens in cystic fibrosis

Thomas Bjarnsholt; Xiaohui Chen Nielsen; Ulla Johansen; Lena Nørgaard; Niels Høiby

doi:10.1007/978-1-61779-120-8_9

Methods to classify bacterial pathogens in cystic fibrosis

Thomas Bjarnsholt, Xiaohui Chen Nielsen, Ulla Johansen, Lena Nørgaard, Niels Høiby

Department of Immunology and Microbiology

6 Citations (Scopus)

Abstract

Many bacteria can be detected in CF sputum, pathogenic and commensal. Modified Koch’s criteria for identification of established and emerging CF pathogens are therefore described. Methods are described to isolate bacteria and to detect bacterial biofilms in sputum or lung tissue from CF patients by means of conventional culturing and staining techniques and by the PNA FISH technique. Additionally, the confocal scanning laser microscopy technique is described for studying biofilms in vitro in a flow cell system. The recA-gene PCR and the RFLP-based identification methods are described for identification of isolates from the Burkholderia complex to the species level. DNA typing by PFGE, which can be used for any bacterial pathogen, is described as it is employed for Pseudomonas aeruginosa. A commercially available ELISA method is described for measuring IgG antibodies against P. aeruginosa in CF patients.

Original language	English
Journal	Methods in Molecular Biology
Volume	742
Pages (from-to)	143-71
Number of pages	29
ISSN	1064-3745
DOIs	https://doi.org/10.1007/978-1-61779-120-8_9
Publication status	Published - 2011

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title = "Methods to classify bacterial pathogens in cystic fibrosis",

abstract = "Many bacteria can be detected in CF sputum, pathogenic and commensal. Modified Koch{\textquoteright}s criteria for identification of established and emerging CF pathogens are therefore described. Methods are described to isolate bacteria and to detect bacterial biofilms in sputum or lung tissue from CF patients by means of conventional culturing and staining techniques and by the PNA FISH technique. Additionally, the confocal scanning laser microscopy technique is described for studying biofilms in vitro in a flow cell system. The recA-gene PCR and the RFLP-based identification methods are described for identification of isolates from the Burkholderia complex to the species level. DNA typing by PFGE, which can be used for any bacterial pathogen, is described as it is employed for Pseudomonas aeruginosa. A commercially available ELISA method is described for measuring IgG antibodies against P. aeruginosa in CF patients.",

author = "Thomas Bjarnsholt and Nielsen, {Xiaohui Chen} and Ulla Johansen and Lena N{\o}rgaard and Niels H{\o}iby",

year = "2011",

doi = "10.1007/978-1-61779-120-8_9",

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volume = "742",

pages = "143--71",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

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TY - JOUR

T1 - Methods to classify bacterial pathogens in cystic fibrosis

AU - Bjarnsholt, Thomas

AU - Nielsen, Xiaohui Chen

AU - Johansen, Ulla

AU - Nørgaard, Lena

AU - Høiby, Niels

PY - 2011

Y1 - 2011

N2 - Many bacteria can be detected in CF sputum, pathogenic and commensal. Modified Koch’s criteria for identification of established and emerging CF pathogens are therefore described. Methods are described to isolate bacteria and to detect bacterial biofilms in sputum or lung tissue from CF patients by means of conventional culturing and staining techniques and by the PNA FISH technique. Additionally, the confocal scanning laser microscopy technique is described for studying biofilms in vitro in a flow cell system. The recA-gene PCR and the RFLP-based identification methods are described for identification of isolates from the Burkholderia complex to the species level. DNA typing by PFGE, which can be used for any bacterial pathogen, is described as it is employed for Pseudomonas aeruginosa. A commercially available ELISA method is described for measuring IgG antibodies against P. aeruginosa in CF patients.

AB - Many bacteria can be detected in CF sputum, pathogenic and commensal. Modified Koch’s criteria for identification of established and emerging CF pathogens are therefore described. Methods are described to isolate bacteria and to detect bacterial biofilms in sputum or lung tissue from CF patients by means of conventional culturing and staining techniques and by the PNA FISH technique. Additionally, the confocal scanning laser microscopy technique is described for studying biofilms in vitro in a flow cell system. The recA-gene PCR and the RFLP-based identification methods are described for identification of isolates from the Burkholderia complex to the species level. DNA typing by PFGE, which can be used for any bacterial pathogen, is described as it is employed for Pseudomonas aeruginosa. A commercially available ELISA method is described for measuring IgG antibodies against P. aeruginosa in CF patients.

U2 - 10.1007/978-1-61779-120-8_9

DO - 10.1007/978-1-61779-120-8_9

M3 - Journal article

C2 - 21547731

SN - 1064-3745

VL - 742

SP - 143

EP - 171

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Methods to classify bacterial pathogens in cystic fibrosis

Abstract

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