TY - JOUR
T1 - Methods for dynamic investigations of surface-attached in vitro bacterial and fungal biofilms
AU - Sternberg, Claus
AU - Bjarnsholt, Thomas
AU - Shirtliff, Mark
PY - 2014
Y1 - 2014
N2 - Three dynamic models for the investigation of in vitro biofilm formation are described in this chapter. In the 6-well plate assay presented here, the placing of the plate on a rotating platform provides shear, thereby making the system dynamic with respect to the static microtiter assay.The second reported model, especially suitable for harvesting high amounts of cells for transcriptomic or proteomic investigations, is based on numerous glass beads placed in a flask incubated with shaking on a rotating platform, thus increasing the surface area for biofilm formation. Finally, the flow-cell system, that is the driving model for elucidating the biofilm-forming process in vitro as well as the biofilm tolerance towards antibiotics and host defense components, is illustrated here.
AB - Three dynamic models for the investigation of in vitro biofilm formation are described in this chapter. In the 6-well plate assay presented here, the placing of the plate on a rotating platform provides shear, thereby making the system dynamic with respect to the static microtiter assay.The second reported model, especially suitable for harvesting high amounts of cells for transcriptomic or proteomic investigations, is based on numerous glass beads placed in a flask incubated with shaking on a rotating platform, thus increasing the surface area for biofilm formation. Finally, the flow-cell system, that is the driving model for elucidating the biofilm-forming process in vitro as well as the biofilm tolerance towards antibiotics and host defense components, is illustrated here.
U2 - 10.1007/978-1-4939-0467-9_1
DO - 10.1007/978-1-4939-0467-9_1
M3 - Journal article
C2 - 24664822
SN - 1064-3745
VL - 1147
SP - 3
EP - 22
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -