Metabolomic analysis of platelets during storage: a comparison between apheresis- and buffy coat-derived platelet concentrates

TY - JOUR

T1 - Metabolomic analysis of platelets during storage

T2 - a comparison between apheresis- and buffy coat-derived platelet concentrates

AU - Paglia, Giuseppe

AU - Sigurjónsson, Ólafur E

AU - Rolfsson, Óttar

AU - Hansen, Morten Bagge

AU - Brynjólfsson, Sigurður

AU - Gudmundsson, Sveinn

AU - Palsson, Bernhard O

N1 - © 2014 AABB.

PY - 2015/2/1

Y1 - 2015/2/1

N2 - BACKGROUND: Platelet concentrates (PCs) can be prepared using three methods: platelet (PLT)-rich plasma, apheresis, and buffy coat. The aim of this study was to obtain a comprehensive data set that describes metabolism of buffy coat-derived PLTs during storage and to compare it with a previously published parallel data set obtained for apheresis-derived PLTs.STUDY DESIGN AND METHODS: During storage we measured more than 150 variables in 8 PLT units, prepared by the buffy coat method. Samples were collected at seven different time points resulting in a data set containing more than 8000 measurements. This data set was obtained by combining a series of standard quality control assays to monitor the quality of stored PLTs and a deep coverage metabolomics study using liquid chromatography coupled with mass spectrometry.RESULTS: Stored PLTs showed a distinct metabolic transition occurring 4 days after their collection. The transition was evident in PLT produced by both production methods. Apheresis-derived PLTs showed a clearer phenotype of PLT activation during early days of storage. The activated phenotype of apheresis PLTs was accompanied by a higher metabolic activity, especially related to glycolysis and the tricarboxylic acid cycle. Moreover, the extent of the activation differed between bags resulting in interbag variability in the storage lesion of apheresis-prepared PLTs. This may be related to donor-related polymorphism.CONCLUSION: This study demonstrated two discrete metabolic phenotypes in stored PLTs prepared with both apheresis and buffy coat methods. PLT activation occurs during the first metabolic phenotype and might lead to a low reproducibility of the apheresis PCs.

AB - BACKGROUND: Platelet concentrates (PCs) can be prepared using three methods: platelet (PLT)-rich plasma, apheresis, and buffy coat. The aim of this study was to obtain a comprehensive data set that describes metabolism of buffy coat-derived PLTs during storage and to compare it with a previously published parallel data set obtained for apheresis-derived PLTs.STUDY DESIGN AND METHODS: During storage we measured more than 150 variables in 8 PLT units, prepared by the buffy coat method. Samples were collected at seven different time points resulting in a data set containing more than 8000 measurements. This data set was obtained by combining a series of standard quality control assays to monitor the quality of stored PLTs and a deep coverage metabolomics study using liquid chromatography coupled with mass spectrometry.RESULTS: Stored PLTs showed a distinct metabolic transition occurring 4 days after their collection. The transition was evident in PLT produced by both production methods. Apheresis-derived PLTs showed a clearer phenotype of PLT activation during early days of storage. The activated phenotype of apheresis PLTs was accompanied by a higher metabolic activity, especially related to glycolysis and the tricarboxylic acid cycle. Moreover, the extent of the activation differed between bags resulting in interbag variability in the storage lesion of apheresis-prepared PLTs. This may be related to donor-related polymorphism.CONCLUSION: This study demonstrated two discrete metabolic phenotypes in stored PLTs prepared with both apheresis and buffy coat methods. PLT activation occurs during the first metabolic phenotype and might lead to a low reproducibility of the apheresis PCs.

KW - Adult

KW - Blood Buffy Coat

KW - Blood Platelets

KW - Blood Preservation

KW - Female

KW - Humans

KW - Male

KW - Metabolome

KW - Metabolomics

KW - Platelet Activation

KW - Plateletpheresis

KW - Time Factors

U2 - 10.1111/trf.12834

DO - 10.1111/trf.12834

M3 - Journal article

C2 - 25156572

SN - 0041-1132

VL - 55

SP - 301

EP - 313

JO - Transfusion

JF - Transfusion

IS - 2

ER -