TY - JOUR
T1 - Metabolism of prostaglandin E and of glutathione conjugate of prostaglandin A (GSH-prostaglandin A) by prostaglandin 9-ketoreductase from rabbit kidney
AU - Toft, B.S.
AU - Hansen, Harald S.
PY - 1979/7/27
Y1 - 1979/7/27
N2 - Rabbit kidney prostaglandin 9-ketoreductase was found to metabolize the glutathione conjugate of prostaglandin A (GSH-prostaglandin A). Apparent K (GSH-prostaglandin A) 13 µM and apparent K (prostaglandin E) 200 µM. The cytosolic preparation was subjected to gelfiltration and isoelectric focusing, which revealed that metabolism of prostaglandin E and GSH-prostaglandin A occurs by means of the same fractions. Furthermore, prostaglandin E and GSH-prostaglandin A are competitive inhibitors of the enzyme, when GSH-prostaglandin A and prostaglandin E are tested as substrates, respectively. It is concluded, that GSH-prostaglandin A is a much better substrate for prostaglandin 9-ketoreductase from rabbit kidney than is prostaglandin E.
AB - Rabbit kidney prostaglandin 9-ketoreductase was found to metabolize the glutathione conjugate of prostaglandin A (GSH-prostaglandin A). Apparent K (GSH-prostaglandin A) 13 µM and apparent K (prostaglandin E) 200 µM. The cytosolic preparation was subjected to gelfiltration and isoelectric focusing, which revealed that metabolism of prostaglandin E and GSH-prostaglandin A occurs by means of the same fractions. Furthermore, prostaglandin E and GSH-prostaglandin A are competitive inhibitors of the enzyme, when GSH-prostaglandin A and prostaglandin E are tested as substrates, respectively. It is concluded, that GSH-prostaglandin A is a much better substrate for prostaglandin 9-ketoreductase from rabbit kidney than is prostaglandin E.
UR - http://www.scopus.com/inward/record.url?scp=0018654510&partnerID=8YFLogxK
M3 - Journal article
AN - SCOPUS:0018654510
SN - 1388-1981
VL - 574
SP - 33
EP - 38
JO - B B A - Molecular and Cell Biology of Lipids
JF - B B A - Molecular and Cell Biology of Lipids
IS - 1
ER -