Membrane transport of anandamide through resealed human red blood cell membranes

I.N. Bojesen, Harald S. Hansen

    38 Citations (Scopus)

    Abstract

    The use of resealed red blood cell membranes (ghosts) allows the study of the transport of a compound in a nonmetabolizing system with a biological membrane. Transmembrane movements of anandamide (N-arachidonoylethanolamine, arachidonoylethanolamide) have been studied by exchange efflux experiments at 0°C and pH 7.3 with albumin-free and albumin-filled human red blood cell ghosts. The efflux kinetics is biexponential and is analyzed in terms of compartment models. The distribution of anandamide on the membrane inner to outer leaflet pools is determined to be 0.275 ± 0.023, and the rate constant of unidirectional flux from inside to outside is 0.361 ± 0.023 s. The rate constant of unidirectional flux from the membrane to BSA in the medium ([BSA]) increases with the square root of [BSA] in accordance with the theory of an unstirred layer around ghosts. Anandamide passed through the red blood cell membrane very rapidly, within seconds. At a molar ratio of anandamide to BSA of
    Original languageEnglish
    JournalJournal of Lipid Research
    Volume46
    Issue number8
    Pages (from-to)1652-1659
    Number of pages8
    ISSN0022-2275
    DOIs
    Publication statusPublished - 1 Aug 2005

    Fingerprint

    Dive into the research topics of 'Membrane transport of anandamide through resealed human red blood cell membranes'. Together they form a unique fingerprint.

    Cite this