Membrane curvature enables N-Ras lipid anchor sorting to liquid-ordered membrane phases

Jannik Bruun Larsen; Martin Borch Jensen; Vikram Kjøller Bhatia; Søren Ljungberg Pedersen; Thomas Bjørnholm; Lars Iversen; Mark Uline; Igal Szleifer; Knud Jørgen Jensen; Nikos Hatzakis; Dimitrios Stamou

doi:10.1038/nchembio.1733

Membrane curvature enables N-Ras lipid anchor sorting to liquid-ordered membrane phases

Jannik Bruun Larsen, Martin Borch Jensen, Vikram Kjøller Bhatia, Søren Ljungberg Pedersen, Thomas Bjørnholm, Lars Iversen, Mark Uline, Igal Szleifer, Knud Jørgen Jensen, Nikos Hatzakis, Dimitrios Stamou

Department of Chemistry

73 Citations (Scopus)

Abstract

Trafficking and sorting of membrane-anchored Ras GTPases are regulated by partitioning between distinct membrane domains. Here, in vitro experiments and microscopic molecular theory reveal membrane curvature as a new modulator of N-Ras lipid anchor and palmitoyl chain partitioning. Membrane curvature was essential for enrichment in raft-like liquid-ordered phases; enrichment was driven by relief of lateral pressure upon anchor insertion and most likely affects the localization of lipidated proteins in general.

Original language	English
Journal	Nature Chemical Biology
Volume	11
Issue number	3
Pages (from-to)	192-194
Number of pages	3
ISSN	1552-4450
DOIs	https://doi.org/10.1038/nchembio.1733
Publication status	Published - Mar 2015

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abstract = "Trafficking and sorting of membrane-anchored Ras GTPases are regulated by partitioning between distinct membrane domains. Here, in vitro experiments and microscopic molecular theory reveal membrane curvature as a new modulator of N-Ras lipid anchor and palmitoyl chain partitioning. Membrane curvature was essential for enrichment in raft-like liquid-ordered phases; enrichment was driven by relief of lateral pressure upon anchor insertion and most likely affects the localization of lipidated proteins in general.",

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AU - Larsen, Jannik Bruun

AU - Jensen, Martin Borch

AU - Bhatia, Vikram Kjøller

AU - Pedersen, Søren Ljungberg

AU - Bjørnholm, Thomas

AU - Iversen, Lars

AU - Uline, Mark

AU - Szleifer, Igal

AU - Jensen, Knud Jørgen

AU - Hatzakis, Nikos

AU - Stamou, Dimitrios

PY - 2015/3

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AB - Trafficking and sorting of membrane-anchored Ras GTPases are regulated by partitioning between distinct membrane domains. Here, in vitro experiments and microscopic molecular theory reveal membrane curvature as a new modulator of N-Ras lipid anchor and palmitoyl chain partitioning. Membrane curvature was essential for enrichment in raft-like liquid-ordered phases; enrichment was driven by relief of lateral pressure upon anchor insertion and most likely affects the localization of lipidated proteins in general.

U2 - 10.1038/nchembio.1733

DO - 10.1038/nchembio.1733

M3 - Journal article

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SN - 1552-4450

VL - 11

SP - 192

EP - 194

JO - Nature Chemical Biology

JF - Nature Chemical Biology

IS - 3

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Membrane curvature enables N-Ras lipid anchor sorting to liquid-ordered membrane phases

Abstract

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