Melon ascorbate oxidase: Cloning of a multigene family, induction during fruit development and repression by wounding

George Diallinas; Eirini Pateraki; Maite Sanmartin; Angela Scossa; Eugenia Stilianou; Nickolas J. Panopoulos; Angelos K. Kanellis

doi:10.1023/A:1005851527227

Melon ascorbate oxidase: Cloning of a multigene family, induction during fruit development and repression by wounding

George Diallinas^*, Eirini Pateraki, Maite Sanmartin, Angela Scossa, Eugenia Stilianou, Nickolas J. Panopoulos, Angelos K. Kanellis

^*Corresponding author for this work

61 Citations (Scopus)

Abstract

A small family of at least four genes encoding melon ascorbate oxidase (AO) has been identified and three members of it have been cloned. Preliminary DNA sequence determination suggested that melon AO genes code for enzymes homologous to ascorbate oxidases from other plants and similar to other multicopper oxidases. We describe detailed molecular studies addressing melon AO expression during organ specific differentiation, fruit development and ripening, and in response to wounding. In particular, AO transcript accumulation was induced in ovaries and the outer mesocarp of mature preclimacteric melon fruits, before the expression of genes encoding the necessary enzymatic activities for ethylene biosynthesis. On the other hand, AO was not expressed in late stages of fruit ripening and was repressed in wounded fruits. The role of ethylene in transcriptional regulation of AO is discussed.

Original language	English
Journal	Plant Molecular Biology
Volume	34
Issue number	5
Pages (from-to)	759-770
Number of pages	12
ISSN	0167-4412
DOIs	https://doi.org/10.1023/A:1005851527227
Publication status	Published - 1 Jul 1997
Externally published	Yes

Keywords

Ascorbate oxidase ethylene
Fruit ripening
Melon (Cucumis melo L.)
Repression
Wounding

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10.1023/A:1005851527227

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@article{fe7867ea73d84fd49022a8a07a20f870,

title = "Melon ascorbate oxidase: Cloning of a multigene family, induction during fruit development and repression by wounding",

abstract = "A small family of at least four genes encoding melon ascorbate oxidase (AO) has been identified and three members of it have been cloned. Preliminary DNA sequence determination suggested that melon AO genes code for enzymes homologous to ascorbate oxidases from other plants and similar to other multicopper oxidases. We describe detailed molecular studies addressing melon AO expression during organ specific differentiation, fruit development and ripening, and in response to wounding. In particular, AO transcript accumulation was induced in ovaries and the outer mesocarp of mature preclimacteric melon fruits, before the expression of genes encoding the necessary enzymatic activities for ethylene biosynthesis. On the other hand, AO was not expressed in late stages of fruit ripening and was repressed in wounded fruits. The role of ethylene in transcriptional regulation of AO is discussed.",

keywords = "Ascorbate oxidase ethylene, Fruit ripening, Melon (Cucumis melo L.), Repression, Wounding",

author = "George Diallinas and Eirini Pateraki and Maite Sanmartin and Angela Scossa and Eugenia Stilianou and Panopoulos, {Nickolas J.} and Kanellis, {Angelos K.}",

year = "1997",

month = jul,

day = "1",

doi = "10.1023/A:1005851527227",

language = "English",

volume = "34",

pages = "759--770",

journal = "Plant Molecular Biology",

issn = "0167-4412",

publisher = "Springer",

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TY - JOUR

T1 - Melon ascorbate oxidase

T2 - Cloning of a multigene family, induction during fruit development and repression by wounding

AU - Diallinas, George

AU - Pateraki, Eirini

AU - Sanmartin, Maite

AU - Scossa, Angela

AU - Stilianou, Eugenia

AU - Panopoulos, Nickolas J.

AU - Kanellis, Angelos K.

PY - 1997/7/1

Y1 - 1997/7/1

N2 - A small family of at least four genes encoding melon ascorbate oxidase (AO) has been identified and three members of it have been cloned. Preliminary DNA sequence determination suggested that melon AO genes code for enzymes homologous to ascorbate oxidases from other plants and similar to other multicopper oxidases. We describe detailed molecular studies addressing melon AO expression during organ specific differentiation, fruit development and ripening, and in response to wounding. In particular, AO transcript accumulation was induced in ovaries and the outer mesocarp of mature preclimacteric melon fruits, before the expression of genes encoding the necessary enzymatic activities for ethylene biosynthesis. On the other hand, AO was not expressed in late stages of fruit ripening and was repressed in wounded fruits. The role of ethylene in transcriptional regulation of AO is discussed.

AB - A small family of at least four genes encoding melon ascorbate oxidase (AO) has been identified and three members of it have been cloned. Preliminary DNA sequence determination suggested that melon AO genes code for enzymes homologous to ascorbate oxidases from other plants and similar to other multicopper oxidases. We describe detailed molecular studies addressing melon AO expression during organ specific differentiation, fruit development and ripening, and in response to wounding. In particular, AO transcript accumulation was induced in ovaries and the outer mesocarp of mature preclimacteric melon fruits, before the expression of genes encoding the necessary enzymatic activities for ethylene biosynthesis. On the other hand, AO was not expressed in late stages of fruit ripening and was repressed in wounded fruits. The role of ethylene in transcriptional regulation of AO is discussed.

KW - Ascorbate oxidase ethylene

KW - Fruit ripening

KW - Melon (Cucumis melo L.)

KW - Repression

KW - Wounding

U2 - 10.1023/A:1005851527227

DO - 10.1023/A:1005851527227

M3 - Journal article

C2 - 9278166

AN - SCOPUS:0031194694

SN - 0167-4412

VL - 34

SP - 759

EP - 770

JO - Plant Molecular Biology

JF - Plant Molecular Biology

IS - 5

ER -

Melon ascorbate oxidase: Cloning of a multigene family, induction during fruit development and repression by wounding

Abstract

Keywords

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