Maternal-fetal hepatic and placental metabolome profiles are associated with reduced fetal growth in a rat model of maternal obesity

Karen Mumme; Clint Gray; Clare M. Reynolds; Mark H. Vickers; Claudia J. Harrison; Joanna L. Stanley; Kathy Ruggiero; Silas G. Villas-Bôas; Philip N. Baker; Karolina Sulek

doi:10.1007/s11306-016-1014-9

Maternal-fetal hepatic and placental metabolome profiles are associated with reduced fetal growth in a rat model of maternal obesity

Karen Mumme, Clint Gray, Clare M. Reynolds, Mark H. Vickers, Claudia J. Harrison, Joanna L. Stanley, Kathy Ruggiero, Silas G. Villas-Bôas, Philip N. Baker, Karolina Sulek^*

^*Corresponding author for this work

3 Citations (Scopus)

Abstract

Introduction: Maternal obesity is associated with a range of pregnancy complications, including fetal growth restriction (FGR), whereby a fetus fails to reach its genetically determined growth. Placental insufficiency and reduced nutrient transport play a role in the onset of FGR. Objectives: Metabolomic profiling was used to reveal altered maternal and fetal metabolic pathways in a model of diet induced obesity during pregnancy, leading to reduced fetal growth. Methods: We examined the metabolome of maternal and fetal livers, and placenta following a high fat and salt intake. Sprague–Dawley rats were assigned to (a) control diet (CD; 1 % salt, 10 % kcal from fat), (b) high salt diet (SD; 4 % salt, 10 % kcal from fat), (c) high fat diet (HF; 1 % salt, 45 % kcal from fat) or (d) high-fat high-salt diet (HFSD; 4 % salt, 45 % kcal from fat) 21 days prior to pregnancy and during gestation. Metabolites from maternal and fetal livers, and placenta were identified using gas and liquid chromatography combined with mass spectrometry. Results: Maternal HF intake resulted in reduced fetal weight. Altered metabolite profiles were observed in the HF maternal and fetal liver, and placenta. Polyunsaturated fatty acid metabolism was significantly altered in maternal and fetal liver by maternal fat intake. Conclusion: Excess of linoleic and α-linoleic acid (essential fatty acids) may be detrimental during placentation and associated with a reduction in fetal weight. Additionally, maternal, placental and fetal response to increased fat consumption seems likely to involve palmitoleic acid utilization as an adaptive response during maternal obesity.

Original language	English
Article number	83
Journal	Metabolomics
Volume	12
Issue number	5
Number of pages	10
ISSN	1573-3882
DOIs	https://doi.org/10.1007/s11306-016-1014-9
Publication status	Published - 2016

Keywords

Fat
Fetal growth restriction
Maternal obesity
Metabolomics
Palmitoleic acid

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s11306-016-1014-9

Cite this

@article{3006d9c3613b458a8f2d3244dc08ea87,

title = "Maternal-fetal hepatic and placental metabolome profiles are associated with reduced fetal growth in a rat model of maternal obesity",

abstract = "Introduction: Maternal obesity is associated with a range of pregnancy complications, including fetal growth restriction (FGR), whereby a fetus fails to reach its genetically determined growth. Placental insufficiency and reduced nutrient transport play a role in the onset of FGR. Objectives: Metabolomic profiling was used to reveal altered maternal and fetal metabolic pathways in a model of diet induced obesity during pregnancy, leading to reduced fetal growth. Methods: We examined the metabolome of maternal and fetal livers, and placenta following a high fat and salt intake. Sprague–Dawley rats were assigned to (a) control diet (CD; 1 % salt, 10 % kcal from fat), (b) high salt diet (SD; 4 % salt, 10 % kcal from fat), (c) high fat diet (HF; 1 % salt, 45 % kcal from fat) or (d) high-fat high-salt diet (HFSD; 4 % salt, 45 % kcal from fat) 21 days prior to pregnancy and during gestation. Metabolites from maternal and fetal livers, and placenta were identified using gas and liquid chromatography combined with mass spectrometry. Results: Maternal HF intake resulted in reduced fetal weight. Altered metabolite profiles were observed in the HF maternal and fetal liver, and placenta. Polyunsaturated fatty acid metabolism was significantly altered in maternal and fetal liver by maternal fat intake. Conclusion: Excess of linoleic and α-linoleic acid (essential fatty acids) may be detrimental during placentation and associated with a reduction in fetal weight. Additionally, maternal, placental and fetal response to increased fat consumption seems likely to involve palmitoleic acid utilization as an adaptive response during maternal obesity.",

keywords = "Fat, Fetal growth restriction, Maternal obesity, Metabolomics, Palmitoleic acid",

author = "Karen Mumme and Clint Gray and Reynolds, {Clare M.} and Vickers, {Mark H.} and Harrison, {Claudia J.} and Stanley, {Joanna L.} and Kathy Ruggiero and Villas-B{\^o}as, {Silas G.} and Baker, {Philip N.} and Karolina Sulek",

year = "2016",

doi = "10.1007/s11306-016-1014-9",

language = "English",

volume = "12",

journal = "Metabolomics",

issn = "1573-3882",

publisher = "Springer",

number = "5",

}

TY - JOUR

T1 - Maternal-fetal hepatic and placental metabolome profiles are associated with reduced fetal growth in a rat model of maternal obesity

AU - Mumme, Karen

AU - Gray, Clint

AU - Reynolds, Clare M.

AU - Vickers, Mark H.

AU - Harrison, Claudia J.

AU - Stanley, Joanna L.

AU - Ruggiero, Kathy

AU - Villas-Bôas, Silas G.

AU - Baker, Philip N.

AU - Sulek, Karolina

PY - 2016

Y1 - 2016

N2 - Introduction: Maternal obesity is associated with a range of pregnancy complications, including fetal growth restriction (FGR), whereby a fetus fails to reach its genetically determined growth. Placental insufficiency and reduced nutrient transport play a role in the onset of FGR. Objectives: Metabolomic profiling was used to reveal altered maternal and fetal metabolic pathways in a model of diet induced obesity during pregnancy, leading to reduced fetal growth. Methods: We examined the metabolome of maternal and fetal livers, and placenta following a high fat and salt intake. Sprague–Dawley rats were assigned to (a) control diet (CD; 1 % salt, 10 % kcal from fat), (b) high salt diet (SD; 4 % salt, 10 % kcal from fat), (c) high fat diet (HF; 1 % salt, 45 % kcal from fat) or (d) high-fat high-salt diet (HFSD; 4 % salt, 45 % kcal from fat) 21 days prior to pregnancy and during gestation. Metabolites from maternal and fetal livers, and placenta were identified using gas and liquid chromatography combined with mass spectrometry. Results: Maternal HF intake resulted in reduced fetal weight. Altered metabolite profiles were observed in the HF maternal and fetal liver, and placenta. Polyunsaturated fatty acid metabolism was significantly altered in maternal and fetal liver by maternal fat intake. Conclusion: Excess of linoleic and α-linoleic acid (essential fatty acids) may be detrimental during placentation and associated with a reduction in fetal weight. Additionally, maternal, placental and fetal response to increased fat consumption seems likely to involve palmitoleic acid utilization as an adaptive response during maternal obesity.

AB - Introduction: Maternal obesity is associated with a range of pregnancy complications, including fetal growth restriction (FGR), whereby a fetus fails to reach its genetically determined growth. Placental insufficiency and reduced nutrient transport play a role in the onset of FGR. Objectives: Metabolomic profiling was used to reveal altered maternal and fetal metabolic pathways in a model of diet induced obesity during pregnancy, leading to reduced fetal growth. Methods: We examined the metabolome of maternal and fetal livers, and placenta following a high fat and salt intake. Sprague–Dawley rats were assigned to (a) control diet (CD; 1 % salt, 10 % kcal from fat), (b) high salt diet (SD; 4 % salt, 10 % kcal from fat), (c) high fat diet (HF; 1 % salt, 45 % kcal from fat) or (d) high-fat high-salt diet (HFSD; 4 % salt, 45 % kcal from fat) 21 days prior to pregnancy and during gestation. Metabolites from maternal and fetal livers, and placenta were identified using gas and liquid chromatography combined with mass spectrometry. Results: Maternal HF intake resulted in reduced fetal weight. Altered metabolite profiles were observed in the HF maternal and fetal liver, and placenta. Polyunsaturated fatty acid metabolism was significantly altered in maternal and fetal liver by maternal fat intake. Conclusion: Excess of linoleic and α-linoleic acid (essential fatty acids) may be detrimental during placentation and associated with a reduction in fetal weight. Additionally, maternal, placental and fetal response to increased fat consumption seems likely to involve palmitoleic acid utilization as an adaptive response during maternal obesity.

KW - Fat

KW - Fetal growth restriction

KW - Maternal obesity

KW - Metabolomics

KW - Palmitoleic acid

U2 - 10.1007/s11306-016-1014-9

DO - 10.1007/s11306-016-1014-9

M3 - Journal article

AN - SCOPUS:84961221231

SN - 1573-3882

VL - 12

JO - Metabolomics

JF - Metabolomics

IS - 5

M1 - 83

ER -

Maternal-fetal hepatic and placental metabolome profiles are associated with reduced fetal growth in a rat model of maternal obesity

Abstract

Keywords

UN SDGs

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