Mapping of the molecular determinants involved in the interaction between eps15 and AP-2.

G Iannolo; A E Salcini; I Gaidarov; O B Goodman; J Baulida; G Carpenter; P G Pelicci; P P Di Fiore; J H Keen

Mapping of the molecular determinants involved in the interaction between eps15 and AP-2.

G Iannolo, A E Salcini, I Gaidarov, O B Goodman, J Baulida, G Carpenter, P G Pelicci, P P Di Fiore, J H Keen

85 Citations (Scopus)

Abstract

eps15, a substrate for the epidermal growth factor receptor and other receptor tyrosine kinases, possesses a discrete domain structure with protein-binding properties. It interacts with a number of cellular proteins through an evolutionarily conserved protein-binding domain, the eps15 homology domain, located in its NH2-terminal region. In addition, a proline-rich region, located in the COOH-terminal portion of eps15, can bind to the Src homology 3 domain of the crk proto-oncogene product in vitro. Recently, coimmunoprecipitation between eps15 and AP-2, a major component of coated pits, was reported. Here, we characterize the molecular determinants of the eps15/AP-2 interaction. The AP-2 binding region of eps15 is localized in its COOH-terminal region and spans approximately 80 amino acids. At least three molecular determinants, located at residues 650-660, 680-690, and 720-730, are involved in the binding. AP-2 binds to eps15 through its alpha subunit (alpha-adaptin); in particular, the COOH-terminal region of alpha-adaptin, the so-called alpha-ear, contains the eps15 binding region.

Original language	English
Journal	Cancer Research
Volume	57
Issue number	2
Pages (from-to)	240-5
Number of pages	5
ISSN	0008-5472
Publication status	Published - 1997

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@article{30a59950519c11dd8d9f000ea68e967b,

title = "Mapping of the molecular determinants involved in the interaction between eps15 and AP-2.",

abstract = "eps15, a substrate for the epidermal growth factor receptor and other receptor tyrosine kinases, possesses a discrete domain structure with protein-binding properties. It interacts with a number of cellular proteins through an evolutionarily conserved protein-binding domain, the eps15 homology domain, located in its NH2-terminal region. In addition, a proline-rich region, located in the COOH-terminal portion of eps15, can bind to the Src homology 3 domain of the crk proto-oncogene product in vitro. Recently, coimmunoprecipitation between eps15 and AP-2, a major component of coated pits, was reported. Here, we characterize the molecular determinants of the eps15/AP-2 interaction. The AP-2 binding region of eps15 is localized in its COOH-terminal region and spans approximately 80 amino acids. At least three molecular determinants, located at residues 650-660, 680-690, and 720-730, are involved in the binding. AP-2 binds to eps15 through its alpha subunit (alpha-adaptin); in particular, the COOH-terminal region of alpha-adaptin, the so-called alpha-ear, contains the eps15 binding region.",

author = "G Iannolo and Salcini, {A E} and I Gaidarov and Goodman, {O B} and J Baulida and G Carpenter and Pelicci, {P G} and {Di Fiore}, {P P} and Keen, {J H}",

note = "Keywords: 3T3 Cells; Adaptor Protein Complex alpha Subunits; Adaptor Proteins, Vesicular Transport; Animals; Base Sequence; Binding Sites; Calcium-Binding Proteins; Membrane Proteins; Mice; Molecular Sequence Data; Peptide Fragments; Peptide Mapping; Phosphoproteins; Recombinant Proteins",

year = "1997",

language = "English",

volume = "57",

pages = "240--5",

journal = "Cancer Research",

issn = "0008-5472",

publisher = "American Association for Cancer Research",

number = "2",

}

TY - JOUR

T1 - Mapping of the molecular determinants involved in the interaction between eps15 and AP-2.

AU - Iannolo, G

AU - Salcini, A E

AU - Gaidarov, I

AU - Goodman, O B

AU - Baulida, J

AU - Carpenter, G

AU - Pelicci, P G

AU - Di Fiore, P P

AU - Keen, J H

N1 - Keywords: 3T3 Cells; Adaptor Protein Complex alpha Subunits; Adaptor Proteins, Vesicular Transport; Animals; Base Sequence; Binding Sites; Calcium-Binding Proteins; Membrane Proteins; Mice; Molecular Sequence Data; Peptide Fragments; Peptide Mapping; Phosphoproteins; Recombinant Proteins

PY - 1997

Y1 - 1997

N2 - eps15, a substrate for the epidermal growth factor receptor and other receptor tyrosine kinases, possesses a discrete domain structure with protein-binding properties. It interacts with a number of cellular proteins through an evolutionarily conserved protein-binding domain, the eps15 homology domain, located in its NH2-terminal region. In addition, a proline-rich region, located in the COOH-terminal portion of eps15, can bind to the Src homology 3 domain of the crk proto-oncogene product in vitro. Recently, coimmunoprecipitation between eps15 and AP-2, a major component of coated pits, was reported. Here, we characterize the molecular determinants of the eps15/AP-2 interaction. The AP-2 binding region of eps15 is localized in its COOH-terminal region and spans approximately 80 amino acids. At least three molecular determinants, located at residues 650-660, 680-690, and 720-730, are involved in the binding. AP-2 binds to eps15 through its alpha subunit (alpha-adaptin); in particular, the COOH-terminal region of alpha-adaptin, the so-called alpha-ear, contains the eps15 binding region.

AB - eps15, a substrate for the epidermal growth factor receptor and other receptor tyrosine kinases, possesses a discrete domain structure with protein-binding properties. It interacts with a number of cellular proteins through an evolutionarily conserved protein-binding domain, the eps15 homology domain, located in its NH2-terminal region. In addition, a proline-rich region, located in the COOH-terminal portion of eps15, can bind to the Src homology 3 domain of the crk proto-oncogene product in vitro. Recently, coimmunoprecipitation between eps15 and AP-2, a major component of coated pits, was reported. Here, we characterize the molecular determinants of the eps15/AP-2 interaction. The AP-2 binding region of eps15 is localized in its COOH-terminal region and spans approximately 80 amino acids. At least three molecular determinants, located at residues 650-660, 680-690, and 720-730, are involved in the binding. AP-2 binds to eps15 through its alpha subunit (alpha-adaptin); in particular, the COOH-terminal region of alpha-adaptin, the so-called alpha-ear, contains the eps15 binding region.

M3 - Journal article

C2 - 9000562

SN - 0008-5472

VL - 57

SP - 240

EP - 245

JO - Cancer Research

JF - Cancer Research

IS - 2

ER -

Mapping of the molecular determinants involved in the interaction between eps15 and AP-2.

Abstract

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