Manipulation of the gut microbiota in C57BL/6 mice changes glucose tolerancewithout affecting weight development and gut mucosal immunity

Gunilla Veslemöy Bech-Nielsen; Camilla Hartmann Friis Hansen; Majbritt Ravn Hufeldt; Dennis Sandris Nielsen; Bent Aasted; Finn Kvist Vogensen; Tore Midtvedt; Axel Jacob Kornerup Hansen

doi:10.1016/j.rvsc.2011.04.005

Manipulation of the gut microbiota in C57BL/6 mice changes glucose tolerancewithout affecting weight development and gut mucosal immunity

Gunilla Veslemöy Bech-Nielsen, Camilla Hartmann Friis Hansen, Majbritt Ravn Hufeldt, Dennis Sandris Nielsen, Bent Aasted, Finn Kvist Vogensen, Tore Midtvedt, Axel Jacob Kornerup Hansen

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Abstract

Inflammatory diseases such as type 2 diabetes (T2D) in humans and mice are under the influence of the composition of the gut microbiota (GM). It was previously demonstrated that treating Lep^ob mice with antibiotics improved glucose tolerance. However, wild type C57BL/6J mice may also exhibit plasma glucose intolerance reminiscent of human T2D. We hypothesized that antibiotic treatment in C57BL/6 mice would have an impact on glucose tolerance without affecting weight and gut immunology. When compared to mice treated with erythromycin or the controls, treatment for five weeks with ampicillin improved glucose tolerance without significantly affecting the weight or the number of gut mucosal regulatory T cells, tolerogenic dendritic cells or T helper cells type 1. 16S rRNA gene based denaturing gradient gel electrophoresis profiles clearly clustered according to treatment and showed that antibiotic treatment reduced GM diversity. It is concluded that antibiotic treatment changes glucose metabolism as well as the composition of the GM in C57BL/6 mice, and that this does not seem to be correlated to weight development in the mice.

Original language	English
Journal	Research in Veterinary Science
Volume	92
Issue number	3
Pages (from-to)	501-508
Number of pages	8
ISSN	0034-5288
DOIs	https://doi.org/10.1016/j.rvsc.2011.04.005
Publication status	Published - 12 Jun 2012

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Bech-Nielsen, G. V., Hansen, C. H. F., Hufeldt, M. R., Nielsen, D. S., Aasted, B., Vogensen, F. K., Midtvedt, T., & Hansen, A. J. K. (2012). Manipulation of the gut microbiota in C57BL/6 mice changes glucose tolerancewithout affecting weight development and gut mucosal immunity. Research in Veterinary Science, 92(3), 501-508. https://doi.org/10.1016/j.rvsc.2011.04.005

Manipulation of the gut microbiota in C57BL/6 mice changes glucose tolerancewithout affecting weight development and gut mucosal immunity. / Bech-Nielsen, Gunilla Veslemöy; Hansen, Camilla Hartmann Friis; Hufeldt, Majbritt Ravn et al.

In: Research in Veterinary Science, Vol. 92, No. 3, 12.06.2012, p. 501-508.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Manipulation of the gut microbiota in C57BL/6 mice changes glucose tolerancewithout affecting weight development and gut mucosal immunity",

abstract = "Inflammatory diseases such as type 2 diabetes (T2D) in humans and mice are under the influence of the composition of the gut microbiota (GM). It was previously demonstrated that treating Lepob mice with antibiotics improved glucose tolerance. However, wild type C57BL/6J mice may also exhibit plasma glucose intolerance reminiscent of human T2D. We hypothesized that antibiotic treatment in C57BL/6 mice would have an impact on glucose tolerance without affecting weight and gut immunology. When compared to mice treated with erythromycin or the controls, treatment for five weeks with ampicillin improved glucose tolerance without significantly affecting the weight or the number of gut mucosal regulatory T cells, tolerogenic dendritic cells or T helper cells type 1. 16S rRNA gene based denaturing gradient gel electrophoresis profiles clearly clustered according to treatment and showed that antibiotic treatment reduced GM diversity. It is concluded that antibiotic treatment changes glucose metabolism as well as the composition of the GM in C57BL/6 mice, and that this does not seem to be correlated to weight development in the mice.",

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AU - Nielsen, Dennis Sandris

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AU - Vogensen, Finn Kvist

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AU - Hansen, Axel Jacob Kornerup

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