Müller cells express the cannabinoid CB2 receptor in the vervet monkey retina

Joseph Bouskila; Pasha Javadi; Christian Casanova; Maurice Ptito; Jean-François Bouchard

doi:10.1002/cne.23333

Müller cells express the cannabinoid CB2 receptor in the vervet monkey retina

Joseph Bouskila, Pasha Javadi, Christian Casanova, Maurice Ptito, Jean-François Bouchard

32 Citations (Scopus)

Abstract

The presence of the cannabinoid receptor type 1 (CB1R) has been largely documented in the rodent and primate retinae in recent years. There is, however, some controversy concerning the presence of the CB2 receptor (CB2R) within the central nervous system. Only recently, CB2R has been found in the rodent retina, but its presence in the primate retina has not yet been demonstrated. The aim of this study was twofold: 1) to characterize the distribution patterns of CB2R in the monkey retina and compare this distribution with that previously reported for CB1R and 2) to resolve the controversy on the presence of CB2R in the neural component of the retina. We therefore thoroughly examined the cellular localization of CB2R in the vervet monkey (Chlorocebus sabeus) retina, using confocal microscopy. Our results demonstrate that CB2R, like CB1R, is present throughout the retinal layers, but with striking dissimilarities. Double labeling of CB2R and glutamine synthetase shows that CB2R is restricted to Müller cell processes, extending from the internal limiting membrane, with very low staining, to the external limiting membrane, with heavy labeling. We conclude that CB2R is indeed present in the retina but exclusively in the retinal glia, whereas CB1R is expressed only in the neuroretina. These results extend our knowledge on the expression and distribution of cannabinoid receptors in the monkey retina, although further experiments are still needed to clarify their role in retinal functions.

Original language	English
Journal	Journal of Comparative Neurology
Volume	521
Issue number	11
Pages (from-to)	2399-415
Number of pages	17
ISSN	0021-9967
DOIs	https://doi.org/10.1002/cne.23333
Publication status	Published - 1 Aug 2013

Keywords

Amacrine Cells
Animals
Antibodies
Blotting, Western
Cannabinoid Receptor Antagonists
Cercopithecus aethiops
Ependymoglial Cells
Excitatory Postsynaptic Potentials
Immunohistochemistry
Microscopy, Confocal
Nerve Tissue Proteins
Neurons
Receptor, Cannabinoid, CB1
Receptor, Cannabinoid, CB2
Retina
Retinal Bipolar Cells
Retinal Cone Photoreceptor Cells
Retinal Ganglion Cells
Retinal Horizontal Cells

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title = "M{\"u}ller cells express the cannabinoid CB2 receptor in the vervet monkey retina",

abstract = "The presence of the cannabinoid receptor type 1 (CB1R) has been largely documented in the rodent and primate retinae in recent years. There is, however, some controversy concerning the presence of the CB2 receptor (CB2R) within the central nervous system. Only recently, CB2R has been found in the rodent retina, but its presence in the primate retina has not yet been demonstrated. The aim of this study was twofold: 1) to characterize the distribution patterns of CB2R in the monkey retina and compare this distribution with that previously reported for CB1R and 2) to resolve the controversy on the presence of CB2R in the neural component of the retina. We therefore thoroughly examined the cellular localization of CB2R in the vervet monkey (Chlorocebus sabeus) retina, using confocal microscopy. Our results demonstrate that CB2R, like CB1R, is present throughout the retinal layers, but with striking dissimilarities. Double labeling of CB2R and glutamine synthetase shows that CB2R is restricted to M{\"u}ller cell processes, extending from the internal limiting membrane, with very low staining, to the external limiting membrane, with heavy labeling. We conclude that CB2R is indeed present in the retina but exclusively in the retinal glia, whereas CB1R is expressed only in the neuroretina. These results extend our knowledge on the expression and distribution of cannabinoid receptors in the monkey retina, although further experiments are still needed to clarify their role in retinal functions.",

keywords = "Amacrine Cells, Animals, Antibodies, Blotting, Western, Cannabinoid Receptor Antagonists, Cercopithecus aethiops, Ependymoglial Cells, Excitatory Postsynaptic Potentials, Immunohistochemistry, Microscopy, Confocal, Nerve Tissue Proteins, Neurons, Receptor, Cannabinoid, CB1, Receptor, Cannabinoid, CB2, Retina, Retinal Bipolar Cells, Retinal Cone Photoreceptor Cells, Retinal Ganglion Cells, Retinal Horizontal Cells",

author = "Joseph Bouskila and Pasha Javadi and Christian Casanova and Maurice Ptito and Jean-Fran{\c c}ois Bouchard",

note = "Copyright {\textcopyright} 2013 Wiley Periodicals, Inc.",

year = "2013",

month = aug,

day = "1",

doi = "10.1002/cne.23333",

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TY - JOUR

T1 - Müller cells express the cannabinoid CB2 receptor in the vervet monkey retina

AU - Bouskila, Joseph

AU - Javadi, Pasha

AU - Casanova, Christian

AU - Ptito, Maurice

AU - Bouchard, Jean-François

PY - 2013/8/1

Y1 - 2013/8/1

N2 - The presence of the cannabinoid receptor type 1 (CB1R) has been largely documented in the rodent and primate retinae in recent years. There is, however, some controversy concerning the presence of the CB2 receptor (CB2R) within the central nervous system. Only recently, CB2R has been found in the rodent retina, but its presence in the primate retina has not yet been demonstrated. The aim of this study was twofold: 1) to characterize the distribution patterns of CB2R in the monkey retina and compare this distribution with that previously reported for CB1R and 2) to resolve the controversy on the presence of CB2R in the neural component of the retina. We therefore thoroughly examined the cellular localization of CB2R in the vervet monkey (Chlorocebus sabeus) retina, using confocal microscopy. Our results demonstrate that CB2R, like CB1R, is present throughout the retinal layers, but with striking dissimilarities. Double labeling of CB2R and glutamine synthetase shows that CB2R is restricted to Müller cell processes, extending from the internal limiting membrane, with very low staining, to the external limiting membrane, with heavy labeling. We conclude that CB2R is indeed present in the retina but exclusively in the retinal glia, whereas CB1R is expressed only in the neuroretina. These results extend our knowledge on the expression and distribution of cannabinoid receptors in the monkey retina, although further experiments are still needed to clarify their role in retinal functions.

AB - The presence of the cannabinoid receptor type 1 (CB1R) has been largely documented in the rodent and primate retinae in recent years. There is, however, some controversy concerning the presence of the CB2 receptor (CB2R) within the central nervous system. Only recently, CB2R has been found in the rodent retina, but its presence in the primate retina has not yet been demonstrated. The aim of this study was twofold: 1) to characterize the distribution patterns of CB2R in the monkey retina and compare this distribution with that previously reported for CB1R and 2) to resolve the controversy on the presence of CB2R in the neural component of the retina. We therefore thoroughly examined the cellular localization of CB2R in the vervet monkey (Chlorocebus sabeus) retina, using confocal microscopy. Our results demonstrate that CB2R, like CB1R, is present throughout the retinal layers, but with striking dissimilarities. Double labeling of CB2R and glutamine synthetase shows that CB2R is restricted to Müller cell processes, extending from the internal limiting membrane, with very low staining, to the external limiting membrane, with heavy labeling. We conclude that CB2R is indeed present in the retina but exclusively in the retinal glia, whereas CB1R is expressed only in the neuroretina. These results extend our knowledge on the expression and distribution of cannabinoid receptors in the monkey retina, although further experiments are still needed to clarify their role in retinal functions.

KW - Amacrine Cells

KW - Animals

KW - Antibodies

KW - Blotting, Western

KW - Cannabinoid Receptor Antagonists

KW - Cercopithecus aethiops

KW - Ependymoglial Cells

KW - Excitatory Postsynaptic Potentials

KW - Immunohistochemistry

KW - Microscopy, Confocal

KW - Nerve Tissue Proteins

KW - Neurons

KW - Receptor, Cannabinoid, CB1

KW - Receptor, Cannabinoid, CB2

KW - Retina

KW - Retinal Bipolar Cells

KW - Retinal Cone Photoreceptor Cells

KW - Retinal Ganglion Cells

KW - Retinal Horizontal Cells

U2 - 10.1002/cne.23333

DO - 10.1002/cne.23333

M3 - Journal article

C2 - 23630038

SN - 0021-9967

VL - 521

SP - 2399

EP - 2415

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

IS - 11

ER -

Müller cells express the cannabinoid CB2 receptor in the vervet monkey retina

Abstract

Keywords

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