Lytic xylan oxidases from wood-decay fungi unlock biomass degradation

Marie Couturier; Simon Ladevèze; Gerlind Sulzenbacher; Luisa Ciano; Mathieu Fanuel; Céline Moreau; Ana Villares; Bernard Cathala; Florence Chaspoul; Kristian E. Frandsen; Aurore Labourel; Isabelle Herpoël-Gimbert; Sacha Grisel; Mireille Haon; Nicolas Lenfant; Hélène Rogniaux; David Ropartz; Gideon J. Davies; Marie Noëlle Rosso; Paul H. Walton; Bernard Henrissat; Jean Guy Berrin

doi:10.1038/nchembio.2558

Lytic xylan oxidases from wood-decay fungi unlock biomass degradation

Marie Couturier, Simon Ladevèze, Gerlind Sulzenbacher, Luisa Ciano, Mathieu Fanuel, Céline Moreau, Ana Villares, Bernard Cathala, Florence Chaspoul, Kristian E. Frandsen, Aurore Labourel, Isabelle Herpoël-Gimbert, Sacha Grisel, Mireille Haon, Nicolas Lenfant, Hélène Rogniaux, David Ropartz, Gideon J. Davies, Marie Noëlle Rosso, Paul H. WaltonBernard Henrissat, Jean Guy Berrin

124 Citations (Scopus)

Abstract

Wood biomass is the most abundant feedstock envisioned for the development of modern biorefineries. However, the cost-effective conversion of this form of biomass into commodity products is limited by its resistance to enzymatic degradation. Here we describe a new family of fungal lytic polysaccharide monooxygenases (LPMOs) prevalent among white-rot and brown-rot basidiomycetes that is active on xylans - a recalcitrant polysaccharide abundant in wood biomass. Two AA14 LPMO members from the white-rot fungus Pycnoporus coccineus substantially increase the efficiency of wood saccharification through oxidative cleavage of highly refractory xylan-coated cellulose fibers. The discovery of this unique enzyme activity advances our knowledge on the degradation of woody biomass in nature and offers an innovative solution for improving enzyme cocktails for biorefinery applications.

Original language	English
Journal	Nature Chemical Biology
Volume	14
Issue number	3
Pages (from-to)	306-310
Number of pages	5
ISSN	1552-4450
DOIs	https://doi.org/10.1038/nchembio.2558
Publication status	Published - 2018
Externally published	Yes

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Couturier, M., Ladevèze, S., Sulzenbacher, G., Ciano, L., Fanuel, M., Moreau, C., Villares, A., Cathala, B., Chaspoul, F., Frandsen, K. E., Labourel, A., Herpoël-Gimbert, I., Grisel, S., Haon, M., Lenfant, N., Rogniaux, H., Ropartz, D., Davies, G. J., Rosso, M. N., ... Berrin, J. G. (2018). Lytic xylan oxidases from wood-decay fungi unlock biomass degradation. Nature Chemical Biology, 14(3), 306-310. https://doi.org/10.1038/nchembio.2558

Couturier, M, Ladevèze, S, Sulzenbacher, G, Ciano, L, Fanuel, M, Moreau, C, Villares, A, Cathala, B, Chaspoul, F, Frandsen, KE, Labourel, A, Herpoël-Gimbert, I, Grisel, S, Haon, M, Lenfant, N, Rogniaux, H, Ropartz, D, Davies, GJ, Rosso, MN, Walton, PH, Henrissat, B & Berrin, JG 2018, 'Lytic xylan oxidases from wood-decay fungi unlock biomass degradation', Nature Chemical Biology, vol. 14, no. 3, pp. 306-310. https://doi.org/10.1038/nchembio.2558

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title = "Lytic xylan oxidases from wood-decay fungi unlock biomass degradation",

abstract = "Wood biomass is the most abundant feedstock envisioned for the development of modern biorefineries. However, the cost-effective conversion of this form of biomass into commodity products is limited by its resistance to enzymatic degradation. Here we describe a new family of fungal lytic polysaccharide monooxygenases (LPMOs) prevalent among white-rot and brown-rot basidiomycetes that is active on xylans - a recalcitrant polysaccharide abundant in wood biomass. Two AA14 LPMO members from the white-rot fungus Pycnoporus coccineus substantially increase the efficiency of wood saccharification through oxidative cleavage of highly refractory xylan-coated cellulose fibers. The discovery of this unique enzyme activity advances our knowledge on the degradation of woody biomass in nature and offers an innovative solution for improving enzyme cocktails for biorefinery applications.",

author = "Marie Couturier and Simon Ladev{\`e}ze and Gerlind Sulzenbacher and Luisa Ciano and Mathieu Fanuel and C{\'e}line Moreau and Ana Villares and Bernard Cathala and Florence Chaspoul and Frandsen, {Kristian E.} and Aurore Labourel and Isabelle Herpo{\"e}l-Gimbert and Sacha Grisel and Mireille Haon and Nicolas Lenfant and H{\'e}l{\`e}ne Rogniaux and David Ropartz and Davies, {Gideon J.} and Rosso, {Marie No{\"e}lle} and Walton, {Paul H.} and Bernard Henrissat and Berrin, {Jean Guy}",

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AU - Couturier, Marie

AU - Ladevèze, Simon

AU - Sulzenbacher, Gerlind

AU - Ciano, Luisa

AU - Fanuel, Mathieu

AU - Moreau, Céline

AU - Villares, Ana

AU - Cathala, Bernard

AU - Chaspoul, Florence

AU - Frandsen, Kristian E.

AU - Labourel, Aurore

AU - Herpoël-Gimbert, Isabelle

AU - Grisel, Sacha

AU - Haon, Mireille

AU - Lenfant, Nicolas

AU - Rogniaux, Hélène

AU - Ropartz, David

AU - Davies, Gideon J.

AU - Rosso, Marie Noëlle

AU - Walton, Paul H.

AU - Henrissat, Bernard

AU - Berrin, Jean Guy

PY - 2018

Y1 - 2018

N2 - Wood biomass is the most abundant feedstock envisioned for the development of modern biorefineries. However, the cost-effective conversion of this form of biomass into commodity products is limited by its resistance to enzymatic degradation. Here we describe a new family of fungal lytic polysaccharide monooxygenases (LPMOs) prevalent among white-rot and brown-rot basidiomycetes that is active on xylans - a recalcitrant polysaccharide abundant in wood biomass. Two AA14 LPMO members from the white-rot fungus Pycnoporus coccineus substantially increase the efficiency of wood saccharification through oxidative cleavage of highly refractory xylan-coated cellulose fibers. The discovery of this unique enzyme activity advances our knowledge on the degradation of woody biomass in nature and offers an innovative solution for improving enzyme cocktails for biorefinery applications.

AB - Wood biomass is the most abundant feedstock envisioned for the development of modern biorefineries. However, the cost-effective conversion of this form of biomass into commodity products is limited by its resistance to enzymatic degradation. Here we describe a new family of fungal lytic polysaccharide monooxygenases (LPMOs) prevalent among white-rot and brown-rot basidiomycetes that is active on xylans - a recalcitrant polysaccharide abundant in wood biomass. Two AA14 LPMO members from the white-rot fungus Pycnoporus coccineus substantially increase the efficiency of wood saccharification through oxidative cleavage of highly refractory xylan-coated cellulose fibers. The discovery of this unique enzyme activity advances our knowledge on the degradation of woody biomass in nature and offers an innovative solution for improving enzyme cocktails for biorefinery applications.

U2 - 10.1038/nchembio.2558

DO - 10.1038/nchembio.2558

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AN - SCOPUS:85042099460

SN - 1552-4450

VL - 14

SP - 306

EP - 310

JO - Nature Chemical Biology

JF - Nature Chemical Biology

IS - 3

ER -

Lytic xylan oxidases from wood-decay fungi unlock biomass degradation

Abstract

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