Lysine acetylation targets protein complexes and co-regulates major cellular functions

TY - JOUR

T1 - Lysine acetylation targets protein complexes and co-regulates major cellular functions

AU - Choudhary, Chuna Ram

AU - Kumar, Chanchal

AU - Gnad, Florian

AU - Nielsen, Michael L

AU - Rehman, Michael

AU - Walther, Tobias C

AU - Olsen, Jesper V

AU - Mann, Matthias

N1 - Keywords: Acetylation; Amino Acid Motifs; Benzamides; Cell Line, Tumor; Cell Nucleus; Cell Physiological Phenomena; Cytoplasm; Enzyme Inhibitors; Histone Deacetylases; Humans; Hydroxamic Acids; Lysine; Mass Spectrometry; Metabolic Networks and Pathways; Mitochondria; Multiprotein Complexes; Protein Processing, Post-Translational; Protein Structure, Tertiary; Proteins; Proteome; Proteomics; Pyridines; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins

PY - 2009

Y1 - 2009

N2 - Lysine acetylation is a reversible posttranslational modification of proteins and plays a key role in regulating gene expression. Technological limitations have so far prevented a global analysis of lysine acetylation's cellular roles. We used high-resolution mass spectrometry to identify 3600 lysine acetylation sites on 1750 proteins and quantified acetylation changes in response to the deacetylase inhibitors suberoylanilide hydroxamic acid and MS-275. Lysine acetylation preferentially targets large macromolecular complexes involved in diverse cellular processes, such as chromatin remodeling, cell cycle, splicing, nuclear transport, and actin nucleation. Acetylation impaired phosphorylation-dependent interactions of 14-3-3 and regulated the yeast cyclin-dependent kinase Cdc28. Our data demonstrate that the regulatory scope of lysine acetylation is broad and comparable with that of other major posttranslational modifications.

AB - Lysine acetylation is a reversible posttranslational modification of proteins and plays a key role in regulating gene expression. Technological limitations have so far prevented a global analysis of lysine acetylation's cellular roles. We used high-resolution mass spectrometry to identify 3600 lysine acetylation sites on 1750 proteins and quantified acetylation changes in response to the deacetylase inhibitors suberoylanilide hydroxamic acid and MS-275. Lysine acetylation preferentially targets large macromolecular complexes involved in diverse cellular processes, such as chromatin remodeling, cell cycle, splicing, nuclear transport, and actin nucleation. Acetylation impaired phosphorylation-dependent interactions of 14-3-3 and regulated the yeast cyclin-dependent kinase Cdc28. Our data demonstrate that the regulatory scope of lysine acetylation is broad and comparable with that of other major posttranslational modifications.

U2 - 10.1126/science.1175371

DO - 10.1126/science.1175371

M3 - Journal article

C2 - 19608861

SN - 0036-8075

VL - 325

SP - 834

EP - 840

JO - Science

JF - Science

IS - 5942

ER -