LPS-induced cytokine production in the monocytic cell line THP-1 determined by multiple quantitative competitive PCR (QC-PCR)

C Glue; J B Hansen; P Schjerling; T Jinquan; Lars K. Poulsen

LPS-induced cytokine production in the monocytic cell line THP-1 determined by multiple quantitative competitive PCR (QC-PCR)

C Glue, J B Hansen, P Schjerling, T Jinquan, Lars K. Poulsen

7 Citations (Scopus)

Abstract

Quantifying cytokines on the protein level can be problematic because of low concentrations or degradation during sample handling. Aiming towards finding a simple method by which to quantify cytokines on the mRNA level, we combined existing and established molecular biology techniques. Based on the principle of quantitative competitive RT-PCR with a DNA-competitor, IL-1beta, IL-6, IL-12alpha and the housekeeping enzyme GAPDH are measured at levels down to 200 copies of mRNA.

Original language	English
Journal	Scandinavian Journal of Clinical & Laboratory Investigation
Volume	62
Issue number	6
Pages (from-to)	405-12
Number of pages	8
ISSN	0036-5513
Publication status	Published - 2002

Keywords

Cell Line
Cytokines
DNA, Complementary
Interleukin-1
Interleukin-12
Interleukin-6
Lipopolysaccharides
Monocytes
Polymerase Chain Reaction
RNA, Messenger

Cite this

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title = "LPS-induced cytokine production in the monocytic cell line THP-1 determined by multiple quantitative competitive PCR (QC-PCR)",

abstract = "Quantifying cytokines on the protein level can be problematic because of low concentrations or degradation during sample handling. Aiming towards finding a simple method by which to quantify cytokines on the mRNA level, we combined existing and established molecular biology techniques. Based on the principle of quantitative competitive RT-PCR with a DNA-competitor, IL-1beta, IL-6, IL-12alpha and the housekeeping enzyme GAPDH are measured at levels down to 200 copies of mRNA.",

keywords = "Cell Line, Cytokines, DNA, Complementary, Interleukin-1, Interleukin-12, Interleukin-6, Lipopolysaccharides, Monocytes, Polymerase Chain Reaction, RNA, Messenger",

author = "C Glue and Hansen, {J B} and P Schjerling and T Jinquan and Poulsen, {Lars K.}",

year = "2002",

language = "English",

volume = "62",

pages = "405--12",

journal = "Scandinavian Journal of Clinical & Laboratory Investigation",

issn = "0036-5513",

publisher = "Taylor & Francis",

number = "6",

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TY - JOUR

T1 - LPS-induced cytokine production in the monocytic cell line THP-1 determined by multiple quantitative competitive PCR (QC-PCR)

AU - Glue, C

AU - Hansen, J B

AU - Schjerling, P

AU - Jinquan, T

AU - Poulsen, Lars K.

PY - 2002

Y1 - 2002

N2 - Quantifying cytokines on the protein level can be problematic because of low concentrations or degradation during sample handling. Aiming towards finding a simple method by which to quantify cytokines on the mRNA level, we combined existing and established molecular biology techniques. Based on the principle of quantitative competitive RT-PCR with a DNA-competitor, IL-1beta, IL-6, IL-12alpha and the housekeeping enzyme GAPDH are measured at levels down to 200 copies of mRNA.

AB - Quantifying cytokines on the protein level can be problematic because of low concentrations or degradation during sample handling. Aiming towards finding a simple method by which to quantify cytokines on the mRNA level, we combined existing and established molecular biology techniques. Based on the principle of quantitative competitive RT-PCR with a DNA-competitor, IL-1beta, IL-6, IL-12alpha and the housekeeping enzyme GAPDH are measured at levels down to 200 copies of mRNA.

KW - Cell Line

KW - Cytokines

KW - DNA, Complementary

KW - Interleukin-1

KW - Interleukin-12

KW - Interleukin-6

KW - Lipopolysaccharides

KW - Monocytes

KW - Polymerase Chain Reaction

KW - RNA, Messenger

M3 - Journal article

C2 - 12469895

SN - 0036-5513

VL - 62

SP - 405

EP - 412

JO - Scandinavian Journal of Clinical & Laboratory Investigation

JF - Scandinavian Journal of Clinical & Laboratory Investigation

IS - 6

ER -

LPS-induced cytokine production in the monocytic cell line THP-1 determined by multiple quantitative competitive PCR (QC-PCR)

Abstract

Keywords

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