Loss of phototaxis and degeneration of an eyespot in long-term algal cultures: evidence from ultrastructure and behaviour in the dinoflagellate Kryptoperidinium foliaceum

TY - JOUR

T1 - Loss of phototaxis and degeneration of an eyespot in long-term algal cultures

T2 - evidence from ultrastructure and behaviour in the dinoflagellate Kryptoperidinium foliaceum

AU - Moldrup, Morten

AU - Moestrup, Øjvind

AU - Hansen, Per Juel

PY - 2013/7

Y1 - 2013/7

N2 - Phototaxis provides phytoplankton with the means to orient themselves in a light gradient. This is accomplished using an eyespot and associated organelles. For the dinoflagellate Kryptoperidinium foliaceum, which has been described as having one of the most elaborate eyespot complexes known, positive phototaxis has hitherto not been reported. In this study, we show that a newly isolated strain of K. foliaceum is indeed capable of positive phototaxis with a mean vector (± 95% confidence interval) of 352°± 2.2, where 0/360° indicates the position of the light source. A study of three strains (UTEX 1688, CCMP 1326, and MBL07) of K. foliaceum showed that the eyespot in two of these strains has degenerated following decades in culture. Thus, previous studies have failed to report positive phototaxis due to loss of directionality caused by the degenerated eyespot. The results are discussed in a broader context and we conclude that studies on algal morphology and physiology may result in erroneous conclusions if based on algal cultures maintained under laboratory conditions for extended periods.

AB - Phototaxis provides phytoplankton with the means to orient themselves in a light gradient. This is accomplished using an eyespot and associated organelles. For the dinoflagellate Kryptoperidinium foliaceum, which has been described as having one of the most elaborate eyespot complexes known, positive phototaxis has hitherto not been reported. In this study, we show that a newly isolated strain of K. foliaceum is indeed capable of positive phototaxis with a mean vector (± 95% confidence interval) of 352°± 2.2, where 0/360° indicates the position of the light source. A study of three strains (UTEX 1688, CCMP 1326, and MBL07) of K. foliaceum showed that the eyespot in two of these strains has degenerated following decades in culture. Thus, previous studies have failed to report positive phototaxis due to loss of directionality caused by the degenerated eyespot. The results are discussed in a broader context and we conclude that studies on algal morphology and physiology may result in erroneous conclusions if based on algal cultures maintained under laboratory conditions for extended periods.

U2 - 10.1111/jeu.12036

DO - 10.1111/jeu.12036

M3 - Journal article

C2 - 23710546

SN - 1066-5234

VL - 60

SP - 327

EP - 334

JO - Journal of Eukaryotic Microbiology

JF - Journal of Eukaryotic Microbiology

IS - 4

ER -