Long-term manure exposure increases soil bacterial community potential for plasmid uptake

TY - JOUR

T1 - Long-term manure exposure increases soil bacterial community potential for plasmid uptake

AU - Musovic, Sanin

AU - Klümper, Uli

AU - Dechesne, Arnaud

AU - Magid, Jakob

AU - Smets, Barth F.

N1 - Special Issue: Microbial Community Ecology

PY - 2014

Y1 - 2014

N2 - Microbial communities derived from soils subject to different agronomic treatments were challenged with three broad host range plasmids, RP4, pIPO2tet and pRO101, via solid surface filter matings to assess their permissiveness. Approximately 1 in 10000 soil bacterial cells could receive and maintain the plasmids. The community permissiveness increased up to 100% in communities derived from manured soil. While the plasmid transfer frequency was significantly influenced by both the type of plasmid and the agronomic treatment, the diversity of the transconjugal pools was purely plasmid dependent and was dominated by β- and γ-Proteobacteria.

AB - Microbial communities derived from soils subject to different agronomic treatments were challenged with three broad host range plasmids, RP4, pIPO2tet and pRO101, via solid surface filter matings to assess their permissiveness. Approximately 1 in 10000 soil bacterial cells could receive and maintain the plasmids. The community permissiveness increased up to 100% in communities derived from manured soil. While the plasmid transfer frequency was significantly influenced by both the type of plasmid and the agronomic treatment, the diversity of the transconjugal pools was purely plasmid dependent and was dominated by β- and γ-Proteobacteria.

U2 - 10.1111/1758-2229.12138

DO - 10.1111/1758-2229.12138

M3 - Journal article

C2 - 24596284

AN - SCOPUS:84897671509

SN - 1758-2229

VL - 6

SP - 125

EP - 130

JO - Environmental Microbiology Reports

JF - Environmental Microbiology Reports

IS - 2

ER -