Long-term industrial metal contamination unexpectedly shaped diversity and activity response of sediment microbiome

TY - JOUR

T1 - Long-term industrial metal contamination unexpectedly shaped diversity and activity response of sediment microbiome

AU - Jacquiod, Samuel Jehan Auguste

AU - Cyriaque, Valentine

AU - Riber, Leise

AU - Al-Soud, Waleed Abu

AU - Gillan, David C.

AU - Wattiez, Ruddy

AU - Sørensen, Søren Johannes

PY - 2018/2/15

Y1 - 2018/2/15

N2 - Metal contamination poses serious biotoxicity and bioaccumulation issues, affecting both abiotic conditions and biological activity in ecosystem trophic levels, especially sediments. The MetalEurop foundry released metals directly into the French river “la Deûle” during a century, contaminating sediments with a 30-fold increase compared to upstream unpolluted areas (Férin, Sensée canal). Previous metaproteogenomic work revealed phylogenetically analogous, but functionally different microbial communities between the two locations. However, their potential activity status in situ remains unknown. The present study respectively compares the structures of both total and active fractions of sediment prokaryotic microbiomes by coupling DNA and RNA-based sequencing approaches at the polluted MetalEurop site and its upstream control. We applied the innovative ecological concept of Functional Response Groups (FRGs) to decipher the adaptive tolerance range of the communities through characterization of microbial lifestyles and strategists. The complementing use of DNA and RNA sequencing revealed indications that metals selected for mechanisms such as microbial facilitation via “public-good” providing bacteria, Horizontal Gene Transfer (HGT) and community coalescence, overall resulting in an unexpected higher microbial diversity at the polluted site.

AB - Metal contamination poses serious biotoxicity and bioaccumulation issues, affecting both abiotic conditions and biological activity in ecosystem trophic levels, especially sediments. The MetalEurop foundry released metals directly into the French river “la Deûle” during a century, contaminating sediments with a 30-fold increase compared to upstream unpolluted areas (Férin, Sensée canal). Previous metaproteogenomic work revealed phylogenetically analogous, but functionally different microbial communities between the two locations. However, their potential activity status in situ remains unknown. The present study respectively compares the structures of both total and active fractions of sediment prokaryotic microbiomes by coupling DNA and RNA-based sequencing approaches at the polluted MetalEurop site and its upstream control. We applied the innovative ecological concept of Functional Response Groups (FRGs) to decipher the adaptive tolerance range of the communities through characterization of microbial lifestyles and strategists. The complementing use of DNA and RNA sequencing revealed indications that metals selected for mechanisms such as microbial facilitation via “public-good” providing bacteria, Horizontal Gene Transfer (HGT) and community coalescence, overall resulting in an unexpected higher microbial diversity at the polluted site.

U2 - 10.1016/j.jhazmat.2017.09.046

DO - 10.1016/j.jhazmat.2017.09.046

M3 - Journal article

C2 - 29055834

SN - 0304-3894

VL - 344

SP - 299

EP - 307

JO - Journal of Hazardous Materials

JF - Journal of Hazardous Materials

ER -