TY - JOUR
T1 - Localization of Ca2+ -activated big-conductance K+ channels in rabbit distal colon.
AU - Hay-Schmidt, Anders
AU - Grunnet, Morten
AU - Abrahamse, Salomon L
AU - Knaus, Hans-Günther
AU - Klaerke, Dan A
N1 - Keywords: Animals; Autoradiography; Basement Membrane; Binding Sites; Colon; Female; Immunohistochemistry; Intestinal Mucosa; Male; Microscopy, Electron; Peptides; Potassium Channels, Calcium-Activated; Rabbits; Radioligand Assay; Rats; Rats, Wistar; Tissue Distribution
PY - 2003
Y1 - 2003
N2 - Big-conductance Ca(2+)-activated K(+) channels (BK channels) may play an important role in the regulation of epithelial salt and water transport, but little is known about the expression level and the precise localization of BK channels in epithelia. The aim of the present study was to quantify and localize the BK channels in the distal colon epithelium by iberiotoxin (IbTX) binding using the radiolabeled iberiotoxin analogue (125)I-IbTX-D19Y/Y36F, by autoradiography and by immunohistochemical studies. The results showed that the surface cells, responsible for Na(+) absorption, contained a high number of BK channels, whereas the abundance of the channels in the Cl(-)-secreting crypt cells was very low or absent. Surprisingly, the (125)I-IbTX-D19Y/Y36F binding and immunohistochemical studies showed expression of BK channels in the apical as well as in the basolateral membranes of the surface cells. In conclusion, the significant and distinct expression of BK channels in epithelia, combined with their strict regulation, indicate that these channels may play an important role in the overall regulation of salt and water transport.
AB - Big-conductance Ca(2+)-activated K(+) channels (BK channels) may play an important role in the regulation of epithelial salt and water transport, but little is known about the expression level and the precise localization of BK channels in epithelia. The aim of the present study was to quantify and localize the BK channels in the distal colon epithelium by iberiotoxin (IbTX) binding using the radiolabeled iberiotoxin analogue (125)I-IbTX-D19Y/Y36F, by autoradiography and by immunohistochemical studies. The results showed that the surface cells, responsible for Na(+) absorption, contained a high number of BK channels, whereas the abundance of the channels in the Cl(-)-secreting crypt cells was very low or absent. Surprisingly, the (125)I-IbTX-D19Y/Y36F binding and immunohistochemical studies showed expression of BK channels in the apical as well as in the basolateral membranes of the surface cells. In conclusion, the significant and distinct expression of BK channels in epithelia, combined with their strict regulation, indicate that these channels may play an important role in the overall regulation of salt and water transport.
U2 - 10.1007/s00424-002-0983-x
DO - 10.1007/s00424-002-0983-x
M3 - Journal article
C2 - 12690464
SN - 0031-6768
VL - 446
SP - 61
EP - 68
JO - Pflügers Archiv - European Journal of Physiology
JF - Pflügers Archiv - European Journal of Physiology
IS - 1
ER -