Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise

U R Mikkelsen; H Langberg; I C Helmark; D Skovgaard; L L Andersen; M Kjaer; Abigail Mackey

doi:10.1152/japplphysiol.00707.2009

Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise

U R Mikkelsen, H Langberg, I C Helmark, D Skovgaard, L L Andersen, M Kjaer, Abigail Mackey

129 Citations (Scopus)

Abstract

Despite the widespread consumption of nonsteroidal anti-inflammatory drugs (NSAIDs), the influence of these drugs on muscle satellite cells is not fully understood. The aim of the present study was to investigate the effect of a local NSAID infusion on satellite cells after unaccustomed eccentric exercise in vivo in human skeletal muscle. Eight young healthy males performed 200 maximal eccentric contractions with each leg. An NSAID was infused via a microdialysis catheter into the vastus lateralis muscle of one leg (NSAID leg) before, during, and for 4.5 h after exercise, with the other leg working as a control (unblocked leg). Muscle biopsies were collected before and 8 days after exercise. Changes in satellite cells and inflammatory cell numbers were investigated by immunohistochemistry. Satellite cells were identified using antibodies against neural cell adhesion molecule and Pax7. The number of Pax7(+) cells per myofiber was increased by 96% on day 8 after exercise in the unblocked leg (0.14 +/- 0.04, mean +/- SE) compared with the prevalue (0.07 +/- 0.02, P < 0.05), whereas the number of Pax7(+) cells was unchanged in the leg muscles exposed to the NSAID (0.07 +/- 0.01). The number of inflammatory cells (CD68(+) or CD16(+) cells) was not significantly increased in either of the legs 8 days after exercise and was unaffected by the NSAID. The main finding in the present study was that the NSAID infusion for 7.5 h during the exercise day suppressed the exercise-induced increase in the number of satellite cells 8 days after exercise. These results suggest that NSAIDs negatively affect satellite cell activity after unaccustomed eccentric exercise.

Original language	English
Journal	Journal of Applied Physiology
Volume	107
Issue number	5
Pages (from-to)	1600-11
Number of pages	12
ISSN	8750-7587
DOIs	https://doi.org/10.1152/japplphysiol.00707.2009
Publication status	Published - Nov 2009

Keywords

Adult
Anti-Inflammatory Agents, Non-Steroidal
Cell Proliferation
Cytokines
Humans
Indomethacin
Infusions, Parenteral
Injections, Intramuscular
Male
Muscle, Skeletal
Physical Endurance
Physical Exertion
Satellite Cells, Skeletal Muscle
Young Adult

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1152/japplphysiol.00707.2009

Cite this

@article{efd719b38b984d5eb5600dae0590ef5c,

title = "Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise",

abstract = "Despite the widespread consumption of nonsteroidal anti-inflammatory drugs (NSAIDs), the influence of these drugs on muscle satellite cells is not fully understood. The aim of the present study was to investigate the effect of a local NSAID infusion on satellite cells after unaccustomed eccentric exercise in vivo in human skeletal muscle. Eight young healthy males performed 200 maximal eccentric contractions with each leg. An NSAID was infused via a microdialysis catheter into the vastus lateralis muscle of one leg (NSAID leg) before, during, and for 4.5 h after exercise, with the other leg working as a control (unblocked leg). Muscle biopsies were collected before and 8 days after exercise. Changes in satellite cells and inflammatory cell numbers were investigated by immunohistochemistry. Satellite cells were identified using antibodies against neural cell adhesion molecule and Pax7. The number of Pax7(+) cells per myofiber was increased by 96% on day 8 after exercise in the unblocked leg (0.14 +/- 0.04, mean +/- SE) compared with the prevalue (0.07 +/- 0.02, P < 0.05), whereas the number of Pax7(+) cells was unchanged in the leg muscles exposed to the NSAID (0.07 +/- 0.01). The number of inflammatory cells (CD68(+) or CD16(+) cells) was not significantly increased in either of the legs 8 days after exercise and was unaffected by the NSAID. The main finding in the present study was that the NSAID infusion for 7.5 h during the exercise day suppressed the exercise-induced increase in the number of satellite cells 8 days after exercise. These results suggest that NSAIDs negatively affect satellite cell activity after unaccustomed eccentric exercise.",

keywords = "Adult, Anti-Inflammatory Agents, Non-Steroidal, Cell Proliferation, Cytokines, Humans, Indomethacin, Infusions, Parenteral, Injections, Intramuscular, Male, Muscle, Skeletal, Physical Endurance, Physical Exertion, Satellite Cells, Skeletal Muscle, Young Adult",

author = "Mikkelsen, {U R} and H Langberg and Helmark, {I C} and D Skovgaard and Andersen, {L L} and M Kjaer and Abigail Mackey",

year = "2009",

month = nov,

doi = "10.1152/japplphysiol.00707.2009",

language = "English",

volume = "107",

pages = "1600--11",

journal = "Journal of Applied Physiology",

issn = "8750-7587",

publisher = "American Physiological Society",

number = "5",

}

TY - JOUR

T1 - Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise

AU - Mikkelsen, U R

AU - Langberg, H

AU - Helmark, I C

AU - Skovgaard, D

AU - Andersen, L L

AU - Kjaer, M

AU - Mackey, Abigail

PY - 2009/11

Y1 - 2009/11

N2 - Despite the widespread consumption of nonsteroidal anti-inflammatory drugs (NSAIDs), the influence of these drugs on muscle satellite cells is not fully understood. The aim of the present study was to investigate the effect of a local NSAID infusion on satellite cells after unaccustomed eccentric exercise in vivo in human skeletal muscle. Eight young healthy males performed 200 maximal eccentric contractions with each leg. An NSAID was infused via a microdialysis catheter into the vastus lateralis muscle of one leg (NSAID leg) before, during, and for 4.5 h after exercise, with the other leg working as a control (unblocked leg). Muscle biopsies were collected before and 8 days after exercise. Changes in satellite cells and inflammatory cell numbers were investigated by immunohistochemistry. Satellite cells were identified using antibodies against neural cell adhesion molecule and Pax7. The number of Pax7(+) cells per myofiber was increased by 96% on day 8 after exercise in the unblocked leg (0.14 +/- 0.04, mean +/- SE) compared with the prevalue (0.07 +/- 0.02, P < 0.05), whereas the number of Pax7(+) cells was unchanged in the leg muscles exposed to the NSAID (0.07 +/- 0.01). The number of inflammatory cells (CD68(+) or CD16(+) cells) was not significantly increased in either of the legs 8 days after exercise and was unaffected by the NSAID. The main finding in the present study was that the NSAID infusion for 7.5 h during the exercise day suppressed the exercise-induced increase in the number of satellite cells 8 days after exercise. These results suggest that NSAIDs negatively affect satellite cell activity after unaccustomed eccentric exercise.

AB - Despite the widespread consumption of nonsteroidal anti-inflammatory drugs (NSAIDs), the influence of these drugs on muscle satellite cells is not fully understood. The aim of the present study was to investigate the effect of a local NSAID infusion on satellite cells after unaccustomed eccentric exercise in vivo in human skeletal muscle. Eight young healthy males performed 200 maximal eccentric contractions with each leg. An NSAID was infused via a microdialysis catheter into the vastus lateralis muscle of one leg (NSAID leg) before, during, and for 4.5 h after exercise, with the other leg working as a control (unblocked leg). Muscle biopsies were collected before and 8 days after exercise. Changes in satellite cells and inflammatory cell numbers were investigated by immunohistochemistry. Satellite cells were identified using antibodies against neural cell adhesion molecule and Pax7. The number of Pax7(+) cells per myofiber was increased by 96% on day 8 after exercise in the unblocked leg (0.14 +/- 0.04, mean +/- SE) compared with the prevalue (0.07 +/- 0.02, P < 0.05), whereas the number of Pax7(+) cells was unchanged in the leg muscles exposed to the NSAID (0.07 +/- 0.01). The number of inflammatory cells (CD68(+) or CD16(+) cells) was not significantly increased in either of the legs 8 days after exercise and was unaffected by the NSAID. The main finding in the present study was that the NSAID infusion for 7.5 h during the exercise day suppressed the exercise-induced increase in the number of satellite cells 8 days after exercise. These results suggest that NSAIDs negatively affect satellite cell activity after unaccustomed eccentric exercise.

KW - Adult

KW - Anti-Inflammatory Agents, Non-Steroidal

KW - Cell Proliferation

KW - Cytokines

KW - Humans

KW - Indomethacin

KW - Infusions, Parenteral

KW - Injections, Intramuscular

KW - Male

KW - Muscle, Skeletal

KW - Physical Endurance

KW - Physical Exertion

KW - Satellite Cells, Skeletal Muscle

KW - Young Adult

U2 - 10.1152/japplphysiol.00707.2009

DO - 10.1152/japplphysiol.00707.2009

M3 - Journal article

C2 - 19713429

SN - 8750-7587

VL - 107

SP - 1600

EP - 1611

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

IS - 5

ER -

Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this