Local and transient permeation events are associated with local melting of giant liposomes

Thomas Andersen; Anders Højbo Kyrsting; Pól Martin Bendix

doi:10.1039/c4sm00410h

Local and transient permeation events are associated with local melting of giant liposomes

Thomas Andersen, Anders Højbo Kyrsting, Pól Martin Bendix

Biocomplexity

20 Citations (Scopus)

Abstract

We reveal that the gel to fluid phase transition causes spherical membrane vesicles to release a finite number of molecules in several consecutive and localized events. By locally melting Giant Unilamellar lipid Vesicles (GUVs), using an optically trapped gold nanoparticle (AuNP) as a local heat source, we establish a local phase transition on the spherical GUV membrane clearly visualized using a phase sensitive fluorescent marker. We measure transient permeation events through this transition zone visualized as de-quenching of calcein as it escapes the interior of the GUV. Since biological membranes share several features with melting membranes, like nanoscale domain formation and critical density fluctuations, similar passive membrane transport could potentially be abundant in living cells.

Original language	English
Journal	Soft Matter
Volume	10
Issue number	24
Pages (from-to)	4268-4274
ISSN	1744-683X
DOIs	https://doi.org/10.1039/c4sm00410h
Publication status	Published - 28 Jun 2014

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10.1039/c4sm00410h

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abstract = "We reveal that the gel to fluid phase transition causes spherical membrane vesicles to release a finite number of molecules in several consecutive and localized events. By locally melting Giant Unilamellar lipid Vesicles (GUVs), using an optically trapped gold nanoparticle (AuNP) as a local heat source, we establish a local phase transition on the spherical GUV membrane clearly visualized using a phase sensitive fluorescent marker. We measure transient permeation events through this transition zone visualized as de-quenching of calcein as it escapes the interior of the GUV. Since biological membranes share several features with melting membranes, like nanoscale domain formation and critical density fluctuations, similar passive membrane transport could potentially be abundant in living cells.",

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T1 - Local and transient permeation events are associated with local melting of giant liposomes

AU - Andersen, Thomas

AU - Kyrsting, Anders Højbo

AU - Bendix, Pól Martin

PY - 2014/6/28

Y1 - 2014/6/28

N2 - We reveal that the gel to fluid phase transition causes spherical membrane vesicles to release a finite number of molecules in several consecutive and localized events. By locally melting Giant Unilamellar lipid Vesicles (GUVs), using an optically trapped gold nanoparticle (AuNP) as a local heat source, we establish a local phase transition on the spherical GUV membrane clearly visualized using a phase sensitive fluorescent marker. We measure transient permeation events through this transition zone visualized as de-quenching of calcein as it escapes the interior of the GUV. Since biological membranes share several features with melting membranes, like nanoscale domain formation and critical density fluctuations, similar passive membrane transport could potentially be abundant in living cells.

AB - We reveal that the gel to fluid phase transition causes spherical membrane vesicles to release a finite number of molecules in several consecutive and localized events. By locally melting Giant Unilamellar lipid Vesicles (GUVs), using an optically trapped gold nanoparticle (AuNP) as a local heat source, we establish a local phase transition on the spherical GUV membrane clearly visualized using a phase sensitive fluorescent marker. We measure transient permeation events through this transition zone visualized as de-quenching of calcein as it escapes the interior of the GUV. Since biological membranes share several features with melting membranes, like nanoscale domain formation and critical density fluctuations, similar passive membrane transport could potentially be abundant in living cells.

U2 - 10.1039/c4sm00410h

DO - 10.1039/c4sm00410h

M3 - Journal article

SN - 1744-683X

VL - 10

SP - 4268

EP - 4274

JO - Soft Matter

JF - Soft Matter

IS - 24

ER -

Local and transient permeation events are associated with local melting of giant liposomes

Abstract

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