Ligand-receptor interactions and membrane structure investigated by AFM and time-resolved fluorescence microscopy

Esben Thormann; Adam C. Simonsen; Lars K. Nielsen; Ole G. Mouritsen

doi:10.1002/jmr.850

Ligand-receptor interactions and membrane structure investigated by AFM and time-resolved fluorescence microscopy

Esben Thormann, Adam C. Simonsen, Lars K. Nielsen, Ole G. Mouritsen^*

^*Corresponding author for this work

18 Citations (Scopus)

Abstract

The atomic force microscope (AFM) and the associated dynamic force spectroscopy technique have been exploited to quantitatively assess the interaction between proteins and their binding to specific ligands and membrane surfaces. In particular, we have studied the specific interaction between lung surfactant protein D and various carbohydrates. In addition, we have used scanning AFM and time-resolved fluorescence microscopy to image the lateral structure of different lipid bilayers and their morphological changes as a function of time. The various systems studied illustrate the potential of modern AFM techniques for application to biomedical research, specifically within immunology and liposome-based drug delivery.

Original language	English
Journal	Journal of Molecular Recognition
Volume	20
Issue number	6
Pages (from-to)	554-560
Number of pages	7
ISSN	0952-3499
DOIs	https://doi.org/10.1002/jmr.850
Publication status	Published - 1 Nov 2007

Keywords

Atomic force microscopy
Force spectroscopy
Lateral membrane structure
Ligand-receptor interaction
Phospholipase A
Single molecules
Surfactant protein D

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10.1002/jmr.850

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title = "Ligand-receptor interactions and membrane structure investigated by AFM and time-resolved fluorescence microscopy",

abstract = "The atomic force microscope (AFM) and the associated dynamic force spectroscopy technique have been exploited to quantitatively assess the interaction between proteins and their binding to specific ligands and membrane surfaces. In particular, we have studied the specific interaction between lung surfactant protein D and various carbohydrates. In addition, we have used scanning AFM and time-resolved fluorescence microscopy to image the lateral structure of different lipid bilayers and their morphological changes as a function of time. The various systems studied illustrate the potential of modern AFM techniques for application to biomedical research, specifically within immunology and liposome-based drug delivery.",

keywords = "Atomic force microscopy, Force spectroscopy, Lateral membrane structure, Ligand-receptor interaction, Phospholipase A, Single molecules, Surfactant protein D",

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AU - Thormann, Esben

AU - Simonsen, Adam C.

AU - Nielsen, Lars K.

AU - Mouritsen, Ole G.

PY - 2007/11/1

Y1 - 2007/11/1

N2 - The atomic force microscope (AFM) and the associated dynamic force spectroscopy technique have been exploited to quantitatively assess the interaction between proteins and their binding to specific ligands and membrane surfaces. In particular, we have studied the specific interaction between lung surfactant protein D and various carbohydrates. In addition, we have used scanning AFM and time-resolved fluorescence microscopy to image the lateral structure of different lipid bilayers and their morphological changes as a function of time. The various systems studied illustrate the potential of modern AFM techniques for application to biomedical research, specifically within immunology and liposome-based drug delivery.

AB - The atomic force microscope (AFM) and the associated dynamic force spectroscopy technique have been exploited to quantitatively assess the interaction between proteins and their binding to specific ligands and membrane surfaces. In particular, we have studied the specific interaction between lung surfactant protein D and various carbohydrates. In addition, we have used scanning AFM and time-resolved fluorescence microscopy to image the lateral structure of different lipid bilayers and their morphological changes as a function of time. The various systems studied illustrate the potential of modern AFM techniques for application to biomedical research, specifically within immunology and liposome-based drug delivery.

KW - Atomic force microscopy

KW - Force spectroscopy

KW - Lateral membrane structure

KW - Ligand-receptor interaction

KW - Phospholipase A

KW - Single molecules

KW - Surfactant protein D

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VL - 20

SP - 554

EP - 560

JO - Journal of Molecular Recognition

JF - Journal of Molecular Recognition

IS - 6

ER -

Ligand-receptor interactions and membrane structure investigated by AFM and time-resolved fluorescence microscopy

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Keywords

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