LIF supports primitive endoderm expansion during pre-implantation development

TY - JOUR

T1 - LIF supports primitive endoderm expansion during pre-implantation development

AU - Morgani, Sophie M

AU - Brickman, Joshua M

N1 - © 2015. Published by The Company of Biologists Ltd.

PY - 2015/10/15

Y1 - 2015/10/15

N2 - Embryonic stem cells (ESCs) are pluripotent cell lines that can be maintained indefinitely in an early developmental state. ESC culture conditions almost always require the cytokine LIF to maintain self-renewal. As ESCs are not homogeneous but contain multiple populations reminiscent of the blastocyst, identifying the target cells of LIF is necessary to understand the propagation of pluripotency. We recently found that LIF acts under self-renewing conditions to stimulate the fraction of ESCs that express extraembryonic markers, but has little impact on pluripotent gene expression. Here, we report that LIF has two distinct roles: it blocks early epiblast (Epi) differentiation, and it supports the expansion of primitive endoderm (PrE)-primed ESCs and PrE in vivo. We find that activation of JAK/STAT signalling downstream of LIF occurs initially throughout the pre-implantation embryo, but later marks the PrE. Moreover, the addition of LIF to cultured embryos increases the GATA6(+) PrE population, whereas inhibition of JAK/STAT signalling reduces both NANOG(+) epiblast and GATA6(+) PrE. The reduction of the NANOG(+) Epi might be explained by its precocious differentiation to later Epi derivatives, whereas the increase in PrE is mediated both by an increase in proliferation and inhibition of PrE apoptosis that is normally triggered in embryos with an excess of GATA6(+) cells. Thus, it appears that the relative size of the PrE is determined by the number of LIF-producing cells in the embryo. This suggests a mechanism by which the embryo adjusts the relative ratio of the primary lineages in response to experimental manipulation.

AB - Embryonic stem cells (ESCs) are pluripotent cell lines that can be maintained indefinitely in an early developmental state. ESC culture conditions almost always require the cytokine LIF to maintain self-renewal. As ESCs are not homogeneous but contain multiple populations reminiscent of the blastocyst, identifying the target cells of LIF is necessary to understand the propagation of pluripotency. We recently found that LIF acts under self-renewing conditions to stimulate the fraction of ESCs that express extraembryonic markers, but has little impact on pluripotent gene expression. Here, we report that LIF has two distinct roles: it blocks early epiblast (Epi) differentiation, and it supports the expansion of primitive endoderm (PrE)-primed ESCs and PrE in vivo. We find that activation of JAK/STAT signalling downstream of LIF occurs initially throughout the pre-implantation embryo, but later marks the PrE. Moreover, the addition of LIF to cultured embryos increases the GATA6(+) PrE population, whereas inhibition of JAK/STAT signalling reduces both NANOG(+) epiblast and GATA6(+) PrE. The reduction of the NANOG(+) Epi might be explained by its precocious differentiation to later Epi derivatives, whereas the increase in PrE is mediated both by an increase in proliferation and inhibition of PrE apoptosis that is normally triggered in embryos with an excess of GATA6(+) cells. Thus, it appears that the relative size of the PrE is determined by the number of LIF-producing cells in the embryo. This suggests a mechanism by which the embryo adjusts the relative ratio of the primary lineages in response to experimental manipulation.

KW - Animals

KW - Apoptosis

KW - Blastocyst

KW - Cell Differentiation

KW - Cell Lineage

KW - Cytokines

KW - Embryonic Development

KW - Embryonic Stem Cells

KW - Endoderm

KW - Female

KW - Flow Cytometry

KW - GATA6 Transcription Factor

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Developmental

KW - Interleukin-6

KW - Janus Kinases

KW - Leukemia Inhibitory Factor

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Microscopy, Confocal

KW - Phenotype

KW - Pluripotent Stem Cells

KW - STAT3 Transcription Factor

KW - Time Factors

U2 - 10.1242/dev.125021

DO - 10.1242/dev.125021

M3 - Journal article

C2 - 26395492

SN - 0950-1991

VL - 142

SP - 3488

EP - 3499

JO - Development

JF - Development

IS - 20

ER -