Laser Doppler flowmetry is valid for measurement of cerebral blood flow autoregulation lower limit in rats

TY - JOUR

T1 - Laser Doppler flowmetry is valid for measurement of cerebral blood flow autoregulation lower limit in rats

AU - Tonnesen, Jan

AU - Pryds, Anders

AU - Larsen, Erik Hviid

AU - Paulson, Olaf B.

AU - Hauerberg, John

AU - Knudsen, Gitte Moos

PY - 2005

Y1 - 2005

N2 - Laser Doppler flowmetry (LDF) is a recent technique that is increasingly being used to monitor relative changes in cerebral blood flow whereas the intra-arterial 133xenon injection technique is a well-established method for repeated absolute measurements of cerebral blood flow. The aim of this study was to validate LDF for assessment of cerebral autoregulation and CO2 reactivity with the 133xenon injection technique as the gold standard. Simultaneous measurements of cerebral blood flow (CBF) were collected by LDF (CBFLDF) and the 133xenon method (CBFXe) while (1) cerebral autoregulation was challenged by controlled systemic haemorrhage, or (2) cerebral blood flow was varied by manipulating the arterial partial pressure of CO2 (Pa,CO2). LDF slightly overestimated CBF under conditions of haemorrhagic shock and haemodilution caused by controlled haemorrhage (paired t test, P < 0.05). However for pooled data, the autoregulation lower limit was similar when determined with the 133xenon and the LDF techniques: 65 ± 3.9 mmHg and 60 ± 5.6 mmHg, respectively. Linear regression analysis yielded CBFXe= (1.02 × CBFLDF) + 9.1 and r= 0.90. Even for substantial changes in Pa,CO2, the two methods resulted in similar results. We conclude that even though LDF overestimated CBF during haemorrhagic shock caused by controlled haemorrhage, the lower limit autoregulation was correctly identified. The laser Doppler technique provides a reliable method for detection of a wide range of cerebral blood flow changes under CO2 challenge. Haemodilution influences the two methods differently causing relative overestimation of blood flow by the laser Doppler technique compared to the 133xenon method.

AB - Laser Doppler flowmetry (LDF) is a recent technique that is increasingly being used to monitor relative changes in cerebral blood flow whereas the intra-arterial 133xenon injection technique is a well-established method for repeated absolute measurements of cerebral blood flow. The aim of this study was to validate LDF for assessment of cerebral autoregulation and CO2 reactivity with the 133xenon injection technique as the gold standard. Simultaneous measurements of cerebral blood flow (CBF) were collected by LDF (CBFLDF) and the 133xenon method (CBFXe) while (1) cerebral autoregulation was challenged by controlled systemic haemorrhage, or (2) cerebral blood flow was varied by manipulating the arterial partial pressure of CO2 (Pa,CO2). LDF slightly overestimated CBF under conditions of haemorrhagic shock and haemodilution caused by controlled haemorrhage (paired t test, P < 0.05). However for pooled data, the autoregulation lower limit was similar when determined with the 133xenon and the LDF techniques: 65 ± 3.9 mmHg and 60 ± 5.6 mmHg, respectively. Linear regression analysis yielded CBFXe= (1.02 × CBFLDF) + 9.1 and r= 0.90. Even for substantial changes in Pa,CO2, the two methods resulted in similar results. We conclude that even though LDF overestimated CBF during haemorrhagic shock caused by controlled haemorrhage, the lower limit autoregulation was correctly identified. The laser Doppler technique provides a reliable method for detection of a wide range of cerebral blood flow changes under CO2 challenge. Haemodilution influences the two methods differently causing relative overestimation of blood flow by the laser Doppler technique compared to the 133xenon method.

U2 - 10.1113/expphysiol.2004.029512

DO - 10.1113/expphysiol.2004.029512

M3 - Journal article

C2 - 15653714

SN - 0958-0670

VL - 90

SP - 349

EP - 355

JO - Experimental Physiology

JF - Experimental Physiology

IS - 3

ER -