Large-scale phenomics identifies primary and fine-tuning roles for CRKs in responses related to oxidative stress

TY - JOUR

T1 - Large-scale phenomics identifies primary and fine-tuning roles for CRKs in responses related to oxidative stress

AU - Bourdais, Gildas

AU - Burdiak, Pawel

AU - Gauthier, Adrien

AU - Nitsch, Lisette

AU - Salojärvi, Jarkko

AU - Rayapuram, Channabasavangowda

AU - Idänheimo, Niina

AU - Hunter, Kerri

AU - Kimura, Sachie

AU - Merilo, Ebe

AU - Vaattovaara, Aleksia

AU - Oracz, Krystyna

AU - Kaufholdt, David

AU - Pallon, Andres

AU - Anggoro, Damar Tri

AU - Glów, Dawid

AU - Lowe, Jennifer

AU - Zhou, Ji

AU - Mohammadi, Omid

AU - Puukko, Tuomas

AU - Albert, Andreas

AU - Lang, Hans

AU - Ernst, Dieter

AU - Kollist, Hannes

AU - Brosché, Mikael

AU - Durner, Jörg

AU - Borst, Jan Willem

AU - Collinge, David B.

AU - Karpinski, Stanislaw

AU - Lyngkjær, Michael Foged

AU - Robatzek, Silke

AU - Wrzaczek, Michael

AU - Kangasjärvi, Jaakko

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Cysteine-rich receptor-like kinases (CRKs) are transmembrane proteins characterized by the presence of two domains of unknown function 26 (DUF26) in their ectodomain. The CRKs form one of the largest groups of receptor-like protein kinases in plants, but their biological functions have so far remained largely uncharacterized. We conducted a large-scale phenotyping approach of a nearly complete crk T-DNA insertion line collection showing that CRKs control important aspects of plant development and stress adaptation in response to biotic and abiotic stimuli in a non-redundant fashion. In particular, the analysis of reactive oxygen species (ROS)-related stress responses, such as regulation of the stomatal aperture, suggests that CRKs participate in ROS/redox signalling and sensing. CRKs play general and fine-tuning roles in the regulation of stomatal closure induced by microbial and abiotic cues. Despite their great number and high similarity, large-scale phenotyping identified specific functions in diverse processes for many CRKs and indicated that CRK2 and CRK5 play predominant roles in growth regulation and stress adaptation, respectively. As a whole, the CRKs contribute to specificity in ROS signalling. Individual CRKs control distinct responses in an antagonistic fashion suggesting future potential for using CRKs in genetic approaches to improve plant performance and stress tolerance.

AB - Cysteine-rich receptor-like kinases (CRKs) are transmembrane proteins characterized by the presence of two domains of unknown function 26 (DUF26) in their ectodomain. The CRKs form one of the largest groups of receptor-like protein kinases in plants, but their biological functions have so far remained largely uncharacterized. We conducted a large-scale phenotyping approach of a nearly complete crk T-DNA insertion line collection showing that CRKs control important aspects of plant development and stress adaptation in response to biotic and abiotic stimuli in a non-redundant fashion. In particular, the analysis of reactive oxygen species (ROS)-related stress responses, such as regulation of the stomatal aperture, suggests that CRKs participate in ROS/redox signalling and sensing. CRKs play general and fine-tuning roles in the regulation of stomatal closure induced by microbial and abiotic cues. Despite their great number and high similarity, large-scale phenotyping identified specific functions in diverse processes for many CRKs and indicated that CRK2 and CRK5 play predominant roles in growth regulation and stress adaptation, respectively. As a whole, the CRKs contribute to specificity in ROS signalling. Individual CRKs control distinct responses in an antagonistic fashion suggesting future potential for using CRKs in genetic approaches to improve plant performance and stress tolerance.

U2 - 10.1371/journal.pgen.1005373

DO - 10.1371/journal.pgen.1005373

M3 - Journal article

C2 - 26197346

SN - 1553-7390

VL - 11

JO - P L o S Genetics

JF - P L o S Genetics

IS - 7

M1 - e1005373

ER -