Kinetics of degradation of dipalmitoylphosphatidylcholine (DPPC) bilayers as a result of vipoxin phospholipase A2 activity: an atomic force microscopy (AFM) approach

TY - JOUR

T1 - Kinetics of degradation of dipalmitoylphosphatidylcholine (DPPC) bilayers as a result of vipoxin phospholipase A2 activity: an atomic force microscopy (AFM) approach

AU - Balashev, Konstantin

AU - Atanasov, Vasil

AU - Mitewa, Mariana

AU - Petrova, Svetla

AU - Bjørnholm, Thomas

N1 - Copyright © 2010 Elsevier B.V. All rights reserved.

PY - 2011/1/1

Y1 - 2011/1/1

N2 - In this paper we used AFM as an analytical tool to visualize the degradation of a phospholipid bilayer undergoing hydrolysis of the vipoxin's PLA(2). We obtained time series images during the degradation process of supported 1, 2-dipalmitoylphosphatidylcholine (DPPC) bilayers and evaluated the occurrence and the growth rate of the bilayer defects. The special resolution of the AFM images allowed us to measure the area and the perimeter length of these defects and to draw conclusions about the kinetics of the enzyme reaction. Moreover, we also report for some unique characteristics discovered during the vipoxin's PLA(2) action. Experimentally for the first time, we observed the appearance and the growth of three-dimensional (3D), crystal-like structures within the formed defects of the degraded bilayer. In an effort to explain their nature, we applied bearing image analysis to estimate the volume of these crystals and we found that their growth rate follows a similar kinetic pattern as the degradation rate of the supported bilayer.

AB - In this paper we used AFM as an analytical tool to visualize the degradation of a phospholipid bilayer undergoing hydrolysis of the vipoxin's PLA(2). We obtained time series images during the degradation process of supported 1, 2-dipalmitoylphosphatidylcholine (DPPC) bilayers and evaluated the occurrence and the growth rate of the bilayer defects. The special resolution of the AFM images allowed us to measure the area and the perimeter length of these defects and to draw conclusions about the kinetics of the enzyme reaction. Moreover, we also report for some unique characteristics discovered during the vipoxin's PLA(2) action. Experimentally for the first time, we observed the appearance and the growth of three-dimensional (3D), crystal-like structures within the formed defects of the degraded bilayer. In an effort to explain their nature, we applied bearing image analysis to estimate the volume of these crystals and we found that their growth rate follows a similar kinetic pattern as the degradation rate of the supported bilayer.

KW - 1,2-Dipalmitoylphosphatidylcholine

KW - Biochemistry

KW - Crystallography, X-Ray

KW - Hydrolysis

KW - Kinetics

KW - Lipid Bilayers

KW - Lipids

KW - Microscopy, Atomic Force

KW - Phospholipases A2

KW - Phospholipids

KW - Protein Conformation

KW - Protein Structure, Tertiary

KW - Time Factors

KW - Viper Venoms

U2 - 10.1016/j.bbamem.2010.10.008

DO - 10.1016/j.bbamem.2010.10.008

M3 - Journal article

C2 - 20959114

SN - 0304-4165

VL - 1808

SP - 191

EP - 198

JO - B B A - General Subjects

JF - B B A - General Subjects

IS - 1

ER -