Jarid2 Is Implicated in the Initial Xist-Induced Targeting of PRC2 to the Inactive X Chromosome

Simão Teixeira da Rocha; Valentina Boeva; Martin Escamilla-Del-Arenal; Katia Ancelin; Camille Granier; Neuza Reis Matias; Serena Sanulli; Jen Chow; Edda Schulz; Christel Picard; Syuzo Kaneko; Kristian Helin; Danny Reinberg; A Francis Stewart; Anton Wutz; Raphaël Margueron; Edith Heard

doi:10.1016/j.molcel.2014.01.002

Jarid2 Is Implicated in the Initial Xist-Induced Targeting of PRC2 to the Inactive X Chromosome

Simão Teixeira da Rocha, Valentina Boeva, Martin Escamilla-Del-Arenal, Katia Ancelin, Camille Granier, Neuza Reis Matias, Serena Sanulli, Jen Chow, Edda Schulz, Christel Picard, Syuzo Kaneko, Kristian Helin, Danny Reinberg, A Francis Stewart, Anton Wutz, Raphaël Margueron, Edith Heard

164 Citations (Scopus)

Abstract

During X chromosome inactivation (XCI), the Polycomb Repressive Complex 2 (PRC2) is thought to participate in the early maintenance of the inactive state. Although Xist RNA is essential for the recruitment of PRC2 to the X chromosome, the precise mechanism remains unclear. Here, we demonstrate that the PRC2 cofactor Jarid2 is an important mediator of Xist-induced PRC2 targeting. The region containing the conserved B and F repeats of Xist is critical for Jarid2 recruitment via its unique N-terminal domain. Xist-induced Jarid2 recruitment occurs chromosome-wide independently of a functional PRC2 complex, unlike at other parts of the genome, such as CG-rich regions, where Jarid2 and PRC2 binding are interdependent. Conversely, we show that Jarid2 loss prevents efficient PRC2 and H3K27me3 enrichment to Xist-coated chromatin. Jarid2 thus represents an important intermediate between PRC2 and Xist RNA for the initial targeting of the PRC2 complex to the X chromosome during onset of XCI.

Original language	English
Journal	Molecular Cell
Volume	53
Issue number	2
Pages (from-to)	301-16
Number of pages	16
ISSN	1097-2765
DOIs	https://doi.org/10.1016/j.molcel.2014.01.002
Publication status	Published - 23 Jan 2014

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da Rocha, S. T., Boeva, V., Escamilla-Del-Arenal, M., Ancelin, K., Granier, C., Matias, N. R., Sanulli, S., Chow, J., Schulz, E., Picard, C., Kaneko, S., Helin, K., Reinberg, D., Stewart, A. F., Wutz, A., Margueron, R., & Heard, E. (2014). Jarid2 Is Implicated in the Initial Xist-Induced Targeting of PRC2 to the Inactive X Chromosome. Molecular Cell, 53(2), 301-16. https://doi.org/10.1016/j.molcel.2014.01.002

da Rocha, ST, Boeva, V, Escamilla-Del-Arenal, M, Ancelin, K, Granier, C, Matias, NR, Sanulli, S, Chow, J, Schulz, E, Picard, C, Kaneko, S, Helin, K, Reinberg, D, Stewart, AF, Wutz, A, Margueron, R & Heard, E 2014, 'Jarid2 Is Implicated in the Initial Xist-Induced Targeting of PRC2 to the Inactive X Chromosome', Molecular Cell, vol. 53, no. 2, pp. 301-16. https://doi.org/10.1016/j.molcel.2014.01.002

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title = "Jarid2 Is Implicated in the Initial Xist-Induced Targeting of PRC2 to the Inactive X Chromosome",

abstract = "During X chromosome inactivation (XCI), the Polycomb Repressive Complex 2 (PRC2) is thought to participate in the early maintenance of the inactive state. Although Xist RNA is essential for the recruitment of PRC2 to the X chromosome, the precise mechanism remains unclear. Here, we demonstrate that the PRC2 cofactor Jarid2 is an important mediator of Xist-induced PRC2 targeting. The region containing the conserved B and F repeats of Xist is critical for Jarid2 recruitment via its unique N-terminal domain. Xist-induced Jarid2 recruitment occurs chromosome-wide independently of a functional PRC2 complex, unlike at other parts of the genome, such as CG-rich regions, where Jarid2 and PRC2 binding are interdependent. Conversely, we show that Jarid2 loss prevents efficient PRC2 and H3K27me3 enrichment to Xist-coated chromatin. Jarid2 thus represents an important intermediate between PRC2 and Xist RNA for the initial targeting of the PRC2 complex to the X chromosome during onset of XCI.",

author = "{da Rocha}, {Sim{\~a}o Teixeira} and Valentina Boeva and Martin Escamilla-Del-Arenal and Katia Ancelin and Camille Granier and Matias, {Neuza Reis} and Serena Sanulli and Jen Chow and Edda Schulz and Christel Picard and Syuzo Kaneko and Kristian Helin and Danny Reinberg and Stewart, {A Francis} and Anton Wutz and Rapha{\"e}l Margueron and Edith Heard",

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T1 - Jarid2 Is Implicated in the Initial Xist-Induced Targeting of PRC2 to the Inactive X Chromosome

AU - da Rocha, Simão Teixeira

AU - Boeva, Valentina

AU - Escamilla-Del-Arenal, Martin

AU - Ancelin, Katia

AU - Granier, Camille

AU - Matias, Neuza Reis

AU - Sanulli, Serena

AU - Chow, Jen

AU - Schulz, Edda

AU - Picard, Christel

AU - Kaneko, Syuzo

AU - Helin, Kristian

AU - Reinberg, Danny

AU - Stewart, A Francis

AU - Wutz, Anton

AU - Margueron, Raphaël

AU - Heard, Edith

PY - 2014/1/23

Y1 - 2014/1/23

N2 - During X chromosome inactivation (XCI), the Polycomb Repressive Complex 2 (PRC2) is thought to participate in the early maintenance of the inactive state. Although Xist RNA is essential for the recruitment of PRC2 to the X chromosome, the precise mechanism remains unclear. Here, we demonstrate that the PRC2 cofactor Jarid2 is an important mediator of Xist-induced PRC2 targeting. The region containing the conserved B and F repeats of Xist is critical for Jarid2 recruitment via its unique N-terminal domain. Xist-induced Jarid2 recruitment occurs chromosome-wide independently of a functional PRC2 complex, unlike at other parts of the genome, such as CG-rich regions, where Jarid2 and PRC2 binding are interdependent. Conversely, we show that Jarid2 loss prevents efficient PRC2 and H3K27me3 enrichment to Xist-coated chromatin. Jarid2 thus represents an important intermediate between PRC2 and Xist RNA for the initial targeting of the PRC2 complex to the X chromosome during onset of XCI.

AB - During X chromosome inactivation (XCI), the Polycomb Repressive Complex 2 (PRC2) is thought to participate in the early maintenance of the inactive state. Although Xist RNA is essential for the recruitment of PRC2 to the X chromosome, the precise mechanism remains unclear. Here, we demonstrate that the PRC2 cofactor Jarid2 is an important mediator of Xist-induced PRC2 targeting. The region containing the conserved B and F repeats of Xist is critical for Jarid2 recruitment via its unique N-terminal domain. Xist-induced Jarid2 recruitment occurs chromosome-wide independently of a functional PRC2 complex, unlike at other parts of the genome, such as CG-rich regions, where Jarid2 and PRC2 binding are interdependent. Conversely, we show that Jarid2 loss prevents efficient PRC2 and H3K27me3 enrichment to Xist-coated chromatin. Jarid2 thus represents an important intermediate between PRC2 and Xist RNA for the initial targeting of the PRC2 complex to the X chromosome during onset of XCI.

U2 - 10.1016/j.molcel.2014.01.002

DO - 10.1016/j.molcel.2014.01.002

M3 - Journal article

C2 - 24462204

SN - 1097-2765

VL - 53

SP - 301

EP - 316

JO - Molecular Cell

JF - Molecular Cell

IS - 2

ER -

Jarid2 Is Implicated in the Initial Xist-Induced Targeting of PRC2 to the Inactive X Chromosome

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