Xanthomonas citri pv. citri pathotypes: LPS structure and function as microbe-associated molecular patterns

TY - JOUR

T1 - Xanthomonas citri pv. citri pathotypes

T2 - LPS structure and function as microbe-associated molecular patterns

AU - Di Lorenzo, Flaviana

AU - Silipo, Alba

AU - Gersby, Lotte Bettina Andersen

AU - Palmigiano, Angelo

AU - Lanzetta, Rosa

AU - Garozzo, Domenico

AU - Boyer, Claudine

AU - Pruvost, Olivier

AU - Newman, Mari-Anne

AU - Molinaro, Antonio

N1 - © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2017/4/18

Y1 - 2017/4/18

N2 - Xanthomonas citri pv. citri is the pathogen responsible for Asiatic citrus canker, one of the most serious citrus diseases worldwide. The lipopolysaccharide (LPS) molecule has been demonstrated to be involved in X. citri pv. citri virulence. Despite enormous progress in investigations of the molecular mechanisms for bacterial pathogenicity, determination of the detailed LPS structure-activity relationship is limited, as the current knowledge is mainly based on structural determination of one X. citri pv. citri strain. As X. citri pv. citri strains are distinguished into three main pathogenicity groups, we characterized the full structure of the LPS from two pathotypes that differ in their host-range specificity. This revealed an intriguing difference in LPS O-chain structure. We also tested the LPSs and isolated lipid A moieties for their ability to act as microbe-associated molecular patterns in Arabidopsis thaliana. Both LPS/lipid As induced ROS accumulation, but no difference was observed between the two pathotypes.

AB - Xanthomonas citri pv. citri is the pathogen responsible for Asiatic citrus canker, one of the most serious citrus diseases worldwide. The lipopolysaccharide (LPS) molecule has been demonstrated to be involved in X. citri pv. citri virulence. Despite enormous progress in investigations of the molecular mechanisms for bacterial pathogenicity, determination of the detailed LPS structure-activity relationship is limited, as the current knowledge is mainly based on structural determination of one X. citri pv. citri strain. As X. citri pv. citri strains are distinguished into three main pathogenicity groups, we characterized the full structure of the LPS from two pathotypes that differ in their host-range specificity. This revealed an intriguing difference in LPS O-chain structure. We also tested the LPSs and isolated lipid A moieties for their ability to act as microbe-associated molecular patterns in Arabidopsis thaliana. Both LPS/lipid As induced ROS accumulation, but no difference was observed between the two pathotypes.

KW - Journal Article

U2 - 10.1002/cbic.201600671

DO - 10.1002/cbic.201600671

M3 - Journal article

C2 - 28186388

SN - 1439-4227

VL - 18

SP - 772

EP - 781

JO - ChemBioChem

JF - ChemBioChem

IS - 8

ER -