TP53 Gene Status Affects Survival in Advanced Mycosis Fungoides

Gitte Wooler; Linea Melchior; Elisabeth Ralfkiaer; Lise Mette Rahbek Gjerdrum; Robert Gniadecki

doi:10.3389/fmed.2016.00051

TP53 Gene Status Affects Survival in Advanced Mycosis Fungoides

Gitte Wooler, Linea Melchior, Elisabeth Ralfkiaer, Lise Mette Rahbek Gjerdrum, Robert Gniadecki

Department of Clinical Medicine

7 Citations (Scopus)

56 Downloads (Pure)

Abstract

TP53 is frequently mutated in different types of neoplasms including leukemia and lymphomas. Mutations of TP53 have also been reported in mycosis fungoides (MF), the most common type of cutaneous lymphoma. However, little is known about the frequency, spectrum of mutations, and their prognostic significance in MF. In this study, we have optimized the protocol for Sanger sequencing of TP53 using DNA extracted from archival paraffin-embedded biopsies. Of 19 samples from patients with stage IIB MF or higher, 31% harbored mutations in TP53. Overall survival of the patients with mutated TP53 was significantly shorter than median survival in the age- and stage-matched patients treated in our Institution. Distribution of mutations was heterogenous in TP53 exons; however, C > T transitions were common suggesting the causal role of ultraviolet radiation. We propose that TP53 mutation status would be useful for risk stratification of patients with advanced MF.

Original language	English
Article number	51
Journal	Frontiers in Medicine
Volume	3
Number of pages	4
ISSN	2296-858X
DOIs	https://doi.org/10.3389/fmed.2016.00051
Publication status	Published - 11 Nov 2016

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TP53 Gene Status Affects Survival in Advanced Mycosis FungoidesFinal published version, 181 KBLicence: CC BY

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title = "TP53 Gene Status Affects Survival in Advanced Mycosis Fungoides",

abstract = "TP53 is frequently mutated in different types of neoplasms including leukemia and lymphomas. Mutations of TP53 have also been reported in mycosis fungoides (MF), the most common type of cutaneous lymphoma. However, little is known about the frequency, spectrum of mutations, and their prognostic significance in MF. In this study, we have optimized the protocol for Sanger sequencing of TP53 using DNA extracted from archival paraffin-embedded biopsies. Of 19 samples from patients with stage IIB MF or higher, 31% harbored mutations in TP53. Overall survival of the patients with mutated TP53 was significantly shorter than median survival in the age- and stage-matched patients treated in our Institution. Distribution of mutations was heterogenous in TP53 exons; however, C > T transitions were common suggesting the causal role of ultraviolet radiation. We propose that TP53 mutation status would be useful for risk stratification of patients with advanced MF.",

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T1 - TP53 Gene Status Affects Survival in Advanced Mycosis Fungoides

AU - Wooler, Gitte

AU - Melchior, Linea

AU - Ralfkiaer, Elisabeth

AU - Rahbek Gjerdrum, Lise Mette

AU - Gniadecki, Robert

PY - 2016/11/11

Y1 - 2016/11/11

N2 - TP53 is frequently mutated in different types of neoplasms including leukemia and lymphomas. Mutations of TP53 have also been reported in mycosis fungoides (MF), the most common type of cutaneous lymphoma. However, little is known about the frequency, spectrum of mutations, and their prognostic significance in MF. In this study, we have optimized the protocol for Sanger sequencing of TP53 using DNA extracted from archival paraffin-embedded biopsies. Of 19 samples from patients with stage IIB MF or higher, 31% harbored mutations in TP53. Overall survival of the patients with mutated TP53 was significantly shorter than median survival in the age- and stage-matched patients treated in our Institution. Distribution of mutations was heterogenous in TP53 exons; however, C > T transitions were common suggesting the causal role of ultraviolet radiation. We propose that TP53 mutation status would be useful for risk stratification of patients with advanced MF.

AB - TP53 is frequently mutated in different types of neoplasms including leukemia and lymphomas. Mutations of TP53 have also been reported in mycosis fungoides (MF), the most common type of cutaneous lymphoma. However, little is known about the frequency, spectrum of mutations, and their prognostic significance in MF. In this study, we have optimized the protocol for Sanger sequencing of TP53 using DNA extracted from archival paraffin-embedded biopsies. Of 19 samples from patients with stage IIB MF or higher, 31% harbored mutations in TP53. Overall survival of the patients with mutated TP53 was significantly shorter than median survival in the age- and stage-matched patients treated in our Institution. Distribution of mutations was heterogenous in TP53 exons; however, C > T transitions were common suggesting the causal role of ultraviolet radiation. We propose that TP53 mutation status would be useful for risk stratification of patients with advanced MF.

KW - Journal Article

U2 - 10.3389/fmed.2016.00051

DO - 10.3389/fmed.2016.00051

M3 - Journal article

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JO - Frontiers in Medicine

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TP53 Gene Status Affects Survival in Advanced Mycosis Fungoides

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