Staphylococcus epidermidis sepsis induces hypercoagulability in preterm pigs

Anne Kirstine Havnsøe Krogh; Anders Brunse; Thomas Thymann; Louise Bochsen; Annemarie T. Kristensen

doi:10.1016/j.rvsc.2019.10.019

Staphylococcus epidermidis sepsis induces hypercoagulability in preterm pigs

Anne Kirstine Havnsøe Krogh^*, Anders Brunse, Thomas Thymann, Louise Bochsen, Annemarie T. Kristensen

^*Corresponding author for this work

Abstract

Gram positive bacteria are a cause of sepsis in human preterm infants, and associates with high mortality and hemostatic dysfunction. It is unknown whether bovine colostrum may protect against sepsis and prevent hemostatic dysfunction. The current study was part of an overall sepsis study investigating Staphylococcus epidermidis (SE) induced sepsis in premature pigs including investigation of the effect of feeding bovine colostrum. The specific hypothesis of this study was that the hemostatic response would be hypercoagulable in septic pigs compared to non-infected controls, and that feeding bovine colostrum would increase the hypercoagulant response. Thromboelastography, activated partial thromboplastin time, prothrombin time and fibrinogen concentration were characterized in SE infected pigs, SE infected pigs fed bovine colostrum, and uninfected controls. All pigs were followed for 24 h. In addition, the same parameters were evaluated in a group of premature pigs and a group of full born pigs all followed for 11 days. SE septic premature pigs were characterized by increased clot strength and decreased fibrinolysis, significantly low platelet count and high fibrinogen concentration. Feeding bovine colostrum did not affect the hemostatic response. Compared to full born pigs, preterm newborn pigs demonstrated reduced clot strength, prolonged prothrombin time and low fibrinogen concentration. In all pigs, the fibrinogen concentration increased 11 days post-partum. To conclude, SE induced sepsis in premature pigs resulted in hypercoagulability. Bovine colostrum did not mitigate the hemostatic response. A hypocoagulable hemostatic response was present in healthy preterm pigs compared to full born pigs, similar to previous reports in infants.

Original language	English
Journal	Research in Veterinary Science
Volume	127
Pages (from-to)	122-129
Number of pages	8
ISSN	0034-5288
DOIs	https://doi.org/10.1016/j.rvsc.2019.10.019
Publication status	Published - 2019

Keywords

Gestational age
Hemostasis
Pig
Preterm animal model
Sepsis
Thromboelastography

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.rvsc.2019.10.019

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title = "Staphylococcus epidermidis sepsis induces hypercoagulability in preterm pigs",

abstract = "Gram positive bacteria are a cause of sepsis in human preterm infants, and associates with high mortality and hemostatic dysfunction. It is unknown whether bovine colostrum may protect against sepsis and prevent hemostatic dysfunction. The current study was part of an overall sepsis study investigating Staphylococcus epidermidis (SE) induced sepsis in premature pigs including investigation of the effect of feeding bovine colostrum. The specific hypothesis of this study was that the hemostatic response would be hypercoagulable in septic pigs compared to non-infected controls, and that feeding bovine colostrum would increase the hypercoagulant response. Thromboelastography, activated partial thromboplastin time, prothrombin time and fibrinogen concentration were characterized in SE infected pigs, SE infected pigs fed bovine colostrum, and uninfected controls. All pigs were followed for 24 h. In addition, the same parameters were evaluated in a group of premature pigs and a group of full born pigs all followed for 11 days. SE septic premature pigs were characterized by increased clot strength and decreased fibrinolysis, significantly low platelet count and high fibrinogen concentration. Feeding bovine colostrum did not affect the hemostatic response. Compared to full born pigs, preterm newborn pigs demonstrated reduced clot strength, prolonged prothrombin time and low fibrinogen concentration. In all pigs, the fibrinogen concentration increased 11 days post-partum. To conclude, SE induced sepsis in premature pigs resulted in hypercoagulability. Bovine colostrum did not mitigate the hemostatic response. A hypocoagulable hemostatic response was present in healthy preterm pigs compared to full born pigs, similar to previous reports in infants.",

keywords = "Gestational age, Hemostasis, Pig, Preterm animal model, Sepsis, Thromboelastography",

author = "Krogh, {Anne Kirstine Havns{\o}e} and Anders Brunse and Thomas Thymann and Louise Bochsen and Kristensen, {Annemarie T.}",

year = "2019",

doi = "10.1016/j.rvsc.2019.10.019",

language = "English",

volume = "127",

pages = "122--129",

journal = "Research in Veterinary Science",

issn = "0034-5288",

publisher = "Elsevier",

}

TY - JOUR

T1 - Staphylococcus epidermidis sepsis induces hypercoagulability in preterm pigs

AU - Krogh, Anne Kirstine Havnsøe

AU - Brunse, Anders

AU - Thymann, Thomas

AU - Bochsen, Louise

AU - Kristensen, Annemarie T.

PY - 2019

Y1 - 2019

N2 - Gram positive bacteria are a cause of sepsis in human preterm infants, and associates with high mortality and hemostatic dysfunction. It is unknown whether bovine colostrum may protect against sepsis and prevent hemostatic dysfunction. The current study was part of an overall sepsis study investigating Staphylococcus epidermidis (SE) induced sepsis in premature pigs including investigation of the effect of feeding bovine colostrum. The specific hypothesis of this study was that the hemostatic response would be hypercoagulable in septic pigs compared to non-infected controls, and that feeding bovine colostrum would increase the hypercoagulant response. Thromboelastography, activated partial thromboplastin time, prothrombin time and fibrinogen concentration were characterized in SE infected pigs, SE infected pigs fed bovine colostrum, and uninfected controls. All pigs were followed for 24 h. In addition, the same parameters were evaluated in a group of premature pigs and a group of full born pigs all followed for 11 days. SE septic premature pigs were characterized by increased clot strength and decreased fibrinolysis, significantly low platelet count and high fibrinogen concentration. Feeding bovine colostrum did not affect the hemostatic response. Compared to full born pigs, preterm newborn pigs demonstrated reduced clot strength, prolonged prothrombin time and low fibrinogen concentration. In all pigs, the fibrinogen concentration increased 11 days post-partum. To conclude, SE induced sepsis in premature pigs resulted in hypercoagulability. Bovine colostrum did not mitigate the hemostatic response. A hypocoagulable hemostatic response was present in healthy preterm pigs compared to full born pigs, similar to previous reports in infants.

AB - Gram positive bacteria are a cause of sepsis in human preterm infants, and associates with high mortality and hemostatic dysfunction. It is unknown whether bovine colostrum may protect against sepsis and prevent hemostatic dysfunction. The current study was part of an overall sepsis study investigating Staphylococcus epidermidis (SE) induced sepsis in premature pigs including investigation of the effect of feeding bovine colostrum. The specific hypothesis of this study was that the hemostatic response would be hypercoagulable in septic pigs compared to non-infected controls, and that feeding bovine colostrum would increase the hypercoagulant response. Thromboelastography, activated partial thromboplastin time, prothrombin time and fibrinogen concentration were characterized in SE infected pigs, SE infected pigs fed bovine colostrum, and uninfected controls. All pigs were followed for 24 h. In addition, the same parameters were evaluated in a group of premature pigs and a group of full born pigs all followed for 11 days. SE septic premature pigs were characterized by increased clot strength and decreased fibrinolysis, significantly low platelet count and high fibrinogen concentration. Feeding bovine colostrum did not affect the hemostatic response. Compared to full born pigs, preterm newborn pigs demonstrated reduced clot strength, prolonged prothrombin time and low fibrinogen concentration. In all pigs, the fibrinogen concentration increased 11 days post-partum. To conclude, SE induced sepsis in premature pigs resulted in hypercoagulability. Bovine colostrum did not mitigate the hemostatic response. A hypocoagulable hemostatic response was present in healthy preterm pigs compared to full born pigs, similar to previous reports in infants.

KW - Gestational age

KW - Hemostasis

KW - Pig

KW - Preterm animal model

KW - Sepsis

KW - Thromboelastography

U2 - 10.1016/j.rvsc.2019.10.019

DO - 10.1016/j.rvsc.2019.10.019

M3 - Journal article

C2 - 31704497

AN - SCOPUS:85074345232

SN - 0034-5288

VL - 127

SP - 122

EP - 129

JO - Research in Veterinary Science

JF - Research in Veterinary Science

ER -

Staphylococcus epidermidis sepsis induces hypercoagulability in preterm pigs

Abstract

Keywords

UN SDGs

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