Isolation of the neuropeptide less than Glu-Trp-Leu-Lys-Gly-Arg-Phe-NH2 (Pol-RFamide II) from the hydromedusa Polyorchis penicillatus

C J Grimmelikhuijzen; K L Rinehart; A N Spencer

Isolation of the neuropeptide less than Glu-Trp-Leu-Lys-Gly-Arg-Phe-NH2 (Pol-RFamide II) from the hydromedusa Polyorchis penicillatus

C J Grimmelikhuijzen, K L Rinehart, A N Spencer

Cell and Neurobiology

19 Citations (Scopus)

Abstract

Using a radioimmunoassay for the sequence Arg-Phe-NH2 (RFamide), we have isolated the peptide less than Glu-Trp-Leu-Lys-Gly-Arg-Phe-NH2 (Pol-RFamide II) from acetic acid extracts of the hydromedusa Polyorchis penicillatus. This peptide is a neuropeptide and constitutes a peptide family together with less than Glu-Leu-Leu-Gly-Gly-Arg-Phe-NH2 (Pol-RFamide I), the first neuropeptide isolated from Polyorchis, and less than Glu-Gly-Arg-Phe-NH2 (Antho-RFamide), a neuropeptide isolated from sea anemones and sea pansies.

Original language	English
Journal	Biochemical and Biophysical Research Communications
Volume	183
Issue number	2
Pages (from-to)	375-82
Number of pages	8
ISSN	0006-291X
Publication status	Published - 16 Mar 1992

Keywords

Amino Acid Sequence
Animals
Gas Chromatography-Mass Spectrometry
Molecular Sequence Data
Neuropeptides
Oligopeptides
Pyrrolidonecarboxylic Acid
Radioimmunoassay
Scyphozoa
Sequence Homology, Nucleic Acid

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Isolation of the neuropeptide less than Glu-Trp-Leu-Lys-Gly-Arg-Phe-NH2 (Pol-RFamide II) from the hydromedusa Polyorchis penicillatus. / Grimmelikhuijzen, C J; Rinehart, K L; Spencer, A N.

In: Biochemical and Biophysical Research Communications, Vol. 183, No. 2, 16.03.1992, p. 375-82.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "Using a radioimmunoassay for the sequence Arg-Phe-NH2 (RFamide), we have isolated the peptide less than Glu-Trp-Leu-Lys-Gly-Arg-Phe-NH2 (Pol-RFamide II) from acetic acid extracts of the hydromedusa Polyorchis penicillatus. This peptide is a neuropeptide and constitutes a peptide family together with less than Glu-Leu-Leu-Gly-Gly-Arg-Phe-NH2 (Pol-RFamide I), the first neuropeptide isolated from Polyorchis, and less than Glu-Gly-Arg-Phe-NH2 (Antho-RFamide), a neuropeptide isolated from sea anemones and sea pansies.",

keywords = "Amino Acid Sequence, Animals, Gas Chromatography-Mass Spectrometry, Molecular Sequence Data, Neuropeptides, Oligopeptides, Pyrrolidonecarboxylic Acid, Radioimmunoassay, Scyphozoa, Sequence Homology, Nucleic Acid",

author = "Grimmelikhuijzen, {C J} and Rinehart, {K L} and Spencer, {A N}",

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T1 - Isolation of the neuropeptide less than Glu-Trp-Leu-Lys-Gly-Arg-Phe-NH2 (Pol-RFamide II) from the hydromedusa Polyorchis penicillatus

AU - Grimmelikhuijzen, C J

AU - Rinehart, K L

AU - Spencer, A N

PY - 1992/3/16

Y1 - 1992/3/16

N2 - Using a radioimmunoassay for the sequence Arg-Phe-NH2 (RFamide), we have isolated the peptide less than Glu-Trp-Leu-Lys-Gly-Arg-Phe-NH2 (Pol-RFamide II) from acetic acid extracts of the hydromedusa Polyorchis penicillatus. This peptide is a neuropeptide and constitutes a peptide family together with less than Glu-Leu-Leu-Gly-Gly-Arg-Phe-NH2 (Pol-RFamide I), the first neuropeptide isolated from Polyorchis, and less than Glu-Gly-Arg-Phe-NH2 (Antho-RFamide), a neuropeptide isolated from sea anemones and sea pansies.

AB - Using a radioimmunoassay for the sequence Arg-Phe-NH2 (RFamide), we have isolated the peptide less than Glu-Trp-Leu-Lys-Gly-Arg-Phe-NH2 (Pol-RFamide II) from acetic acid extracts of the hydromedusa Polyorchis penicillatus. This peptide is a neuropeptide and constitutes a peptide family together with less than Glu-Leu-Leu-Gly-Gly-Arg-Phe-NH2 (Pol-RFamide I), the first neuropeptide isolated from Polyorchis, and less than Glu-Gly-Arg-Phe-NH2 (Antho-RFamide), a neuropeptide isolated from sea anemones and sea pansies.

KW - Amino Acid Sequence

KW - Animals

KW - Gas Chromatography-Mass Spectrometry

KW - Molecular Sequence Data

KW - Neuropeptides

KW - Oligopeptides

KW - Pyrrolidonecarboxylic Acid

KW - Radioimmunoassay

KW - Scyphozoa

KW - Sequence Homology, Nucleic Acid

M3 - Journal article

C2 - 1550547

SN - 0006-291X

VL - 183

SP - 375

EP - 382

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Isolation of the neuropeptide less than Glu-Trp-Leu-Lys-Gly-Arg-Phe-NH2 (Pol-RFamide II) from the hydromedusa Polyorchis penicillatus

Abstract

Keywords

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