Isolation of microRNA targets using biotinylated synthetic microRNAs.

Ulf Andersson Ørom; Anders H Lund

doi:10.1016/j.ymeth.2007.04.007

Isolation of microRNA targets using biotinylated synthetic microRNAs.

Ulf Andersson Ørom, Anders H Lund

124 Citations (Scopus)

Abstract

MicroRNAs are small regulatory RNAs found in multicellular organisms where they post-transcriptionally regulate gene expression. In animals, microRNAs bind mRNAs via incomplete base pairings making the identification of microRNA targets inherently difficult. Here, we present a detailed method for experimental identification of microRNA targets based on affinity purification of tagged microRNAs associated with their targets.
Udgivelsesdato: 2007-Oct

Original language	English
Journal	Methods
Volume	43
Issue number	2
Pages (from-to)	162-5
Number of pages	3
ISSN	1046-2023
DOIs	https://doi.org/10.1016/j.ymeth.2007.04.007
Publication status	Published - 2007

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10.1016/j.ymeth.2007.04.007

Cite this

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title = "Isolation of microRNA targets using biotinylated synthetic microRNAs.",

abstract = "MicroRNAs are small regulatory RNAs found in multicellular organisms where they post-transcriptionally regulate gene expression. In animals, microRNAs bind mRNAs via incomplete base pairings making the identification of microRNA targets inherently difficult. Here, we present a detailed method for experimental identification of microRNA targets based on affinity purification of tagged microRNAs associated with their targets. Udgivelsesdato: 2007-Oct",

author = "{\O}rom, {Ulf Andersson} and Lund, {Anders H}",

note = "Keywords: Animals; Base Sequence; Biotin; Cell Line; Chromatography, Affinity; Drosophila; Drosophila Proteins; Genes, Insect; Humans; MicroRNAs; Neuropeptides; Transfection",

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AU - Ørom, Ulf Andersson

AU - Lund, Anders H

N1 - Keywords: Animals; Base Sequence; Biotin; Cell Line; Chromatography, Affinity; Drosophila; Drosophila Proteins; Genes, Insect; Humans; MicroRNAs; Neuropeptides; Transfection

PY - 2007

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N2 - MicroRNAs are small regulatory RNAs found in multicellular organisms where they post-transcriptionally regulate gene expression. In animals, microRNAs bind mRNAs via incomplete base pairings making the identification of microRNA targets inherently difficult. Here, we present a detailed method for experimental identification of microRNA targets based on affinity purification of tagged microRNAs associated with their targets. Udgivelsesdato: 2007-Oct

AB - MicroRNAs are small regulatory RNAs found in multicellular organisms where they post-transcriptionally regulate gene expression. In animals, microRNAs bind mRNAs via incomplete base pairings making the identification of microRNA targets inherently difficult. Here, we present a detailed method for experimental identification of microRNA targets based on affinity purification of tagged microRNAs associated with their targets. Udgivelsesdato: 2007-Oct

U2 - 10.1016/j.ymeth.2007.04.007

DO - 10.1016/j.ymeth.2007.04.007

M3 - Journal article

C2 - 17889804

SN - 1046-2023

VL - 43

SP - 162

EP - 165

JO - Methods

JF - Methods

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ER -