Isolation of linoleic and alpha-linolenic acids as COX-1 and -2 inhibitors in rose hip

Anna Jäger; K N Petersen; G. Thomasen; S Brøgger Christensen

doi:10.1002/ptr.2446

Isolation of linoleic and alpha-linolenic acids as COX-1 and -2 inhibitors in rose hip

Anna Jäger, K N Petersen, G. Thomasen, S Brøgger Christensen

30 Citations (Scopus)

Abstract

Rose hip has previously shown clinical efficacy in the treatment of osteoarthritis, and organic solvent extracts of rose hip have showed inhibition of cyclooxygenase-1 and -2. A petroleum ether extract of rose hip was fractioned by VLC on silica; on a C-18 column and by HPLC. Each step was COX-1/2 activity-guided. The bioassay-guided fractionation led to the isolation of linoleic acid (the IC50 for COX-1 was 85 microm and 0.6 microM for COX-2) and alpha-linolenic acid (the IC50 for COX-1 was 52 microM and 12 microM for COX-2). The COX-2/COX-1 ratio was 0.007 for linoleic acid and 0.2 for alpha-linolenic acid. Linoleic acid and alpha-linolenic acid contribute to the COX-1 and -2 inhibitory activity of rose hip.

Original language	English
Journal	Phytotherapy Research
Volume	22
Issue number	7
Pages (from-to)	982-984
Number of pages	3
ISSN	0951-418X
DOIs	https://doi.org/10.1002/ptr.2446
Publication status	Published - 2008

Keywords

Chromatography, High Pressure Liquid
Cyclooxygenase 2 Inhibitors
Humans
Linoleic Acid
Osteoarthritis
Phytotherapy
Plant Extracts
Rosa
Vacuum
alpha-Linolenic Acid

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10.1002/ptr.2446

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@article{edc1b8e05a3711dd8d9f000ea68e967b,

title = "Isolation of linoleic and alpha-linolenic acids as COX-1 and -2 inhibitors in rose hip",

abstract = "Rose hip has previously shown clinical efficacy in the treatment of osteoarthritis, and organic solvent extracts of rose hip have showed inhibition of cyclooxygenase-1 and -2. A petroleum ether extract of rose hip was fractioned by VLC on silica; on a C-18 column and by HPLC. Each step was COX-1/2 activity-guided. The bioassay-guided fractionation led to the isolation of linoleic acid (the IC50 for COX-1 was 85 microm and 0.6 microM for COX-2) and alpha-linolenic acid (the IC50 for COX-1 was 52 microM and 12 microM for COX-2). The COX-2/COX-1 ratio was 0.007 for linoleic acid and 0.2 for alpha-linolenic acid. Linoleic acid and alpha-linolenic acid contribute to the COX-1 and -2 inhibitory activity of rose hip.",

keywords = "Chromatography, High Pressure Liquid, Cyclooxygenase 2 Inhibitors, Humans, Linoleic Acid, Osteoarthritis, Phytotherapy, Plant Extracts, Rosa, Vacuum, alpha-Linolenic Acid",

author = "Anna J{\"a}ger and Petersen, {K N} and G. Thomasen and Christensen, {S Br{\o}gger}",

note = "Keywords: cyclooxygenase; linoleic acid; a-linolenic acid; Rosa canina; rose hip",

year = "2008",

doi = "10.1002/ptr.2446",

language = "English",

volume = "22",

pages = "982--984",

journal = "Phytotherapy Research",

issn = "0951-418X",

publisher = "JohnWiley & Sons Ltd",

number = "7",

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TY - JOUR

T1 - Isolation of linoleic and alpha-linolenic acids as COX-1 and -2 inhibitors in rose hip

AU - Jäger, Anna

AU - Petersen, K N

AU - Thomasen, G.

AU - Christensen, S Brøgger

N1 - Keywords: cyclooxygenase; linoleic acid; a-linolenic acid; Rosa canina; rose hip

PY - 2008

Y1 - 2008

N2 - Rose hip has previously shown clinical efficacy in the treatment of osteoarthritis, and organic solvent extracts of rose hip have showed inhibition of cyclooxygenase-1 and -2. A petroleum ether extract of rose hip was fractioned by VLC on silica; on a C-18 column and by HPLC. Each step was COX-1/2 activity-guided. The bioassay-guided fractionation led to the isolation of linoleic acid (the IC50 for COX-1 was 85 microm and 0.6 microM for COX-2) and alpha-linolenic acid (the IC50 for COX-1 was 52 microM and 12 microM for COX-2). The COX-2/COX-1 ratio was 0.007 for linoleic acid and 0.2 for alpha-linolenic acid. Linoleic acid and alpha-linolenic acid contribute to the COX-1 and -2 inhibitory activity of rose hip.

AB - Rose hip has previously shown clinical efficacy in the treatment of osteoarthritis, and organic solvent extracts of rose hip have showed inhibition of cyclooxygenase-1 and -2. A petroleum ether extract of rose hip was fractioned by VLC on silica; on a C-18 column and by HPLC. Each step was COX-1/2 activity-guided. The bioassay-guided fractionation led to the isolation of linoleic acid (the IC50 for COX-1 was 85 microm and 0.6 microM for COX-2) and alpha-linolenic acid (the IC50 for COX-1 was 52 microM and 12 microM for COX-2). The COX-2/COX-1 ratio was 0.007 for linoleic acid and 0.2 for alpha-linolenic acid. Linoleic acid and alpha-linolenic acid contribute to the COX-1 and -2 inhibitory activity of rose hip.

KW - Chromatography, High Pressure Liquid

KW - Cyclooxygenase 2 Inhibitors

KW - Humans

KW - Linoleic Acid

KW - Osteoarthritis

KW - Phytotherapy

KW - Plant Extracts

KW - Rosa

KW - Vacuum

KW - alpha-Linolenic Acid

U2 - 10.1002/ptr.2446

DO - 10.1002/ptr.2446

M3 - Journal article

C2 - 18389471

SN - 0951-418X

VL - 22

SP - 982

EP - 984

JO - Phytotherapy Research

JF - Phytotherapy Research

IS - 7

ER -

Isolation of linoleic and alpha-linolenic acids as COX-1 and -2 inhibitors in rose hip

Abstract

Keywords

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