Penium margaritaceum as a model organism for cell wall analysis of expanding plant cells

Maja Gro Rydahl; Jonatan Ulrik Fangel; Maria Dalgaard Mikkelsen; Ida Elisabeth Johansen; Amanda Andreas; Jesper Harholt; Peter Ulvskov; Bodil Jørgensen; David S. Domozych; William George Tycho Willats

doi:10.1007/978-1-4939-1902-4_1

Penium margaritaceum as a model organism for cell wall analysis of expanding plant cells

Maja Gro Rydahl^*, Jonatan Ulrik Fangel, Maria Dalgaard Mikkelsen, Ida Elisabeth Johansen, Amanda Andreas, Jesper Harholt, Peter Ulvskov, Bodil Jørgensen, David S. Domozych, William George Tycho Willats

^*Corresponding author for this work

Section for Plant Glycobiology

5 Citations (Scopus)

Abstract

The growth of a plant cell encompasses a complex set of subcellular components interacting in a highly coordinated fashion. Ultimately, these activities create specific cell wall structural domains that regulate the prime force of expansion, internally generated turgor pressure. The precise organization of the polymeric networks of the cell wall around the protoplast also contributes to the direction of growth, the shape of the cell, and the proper positioning of the cell in a tissue. In essence, plant cell expansion represents the foundation of development. Most studies of plant cell expansion have focused primarily upon late divergent multicellular land plants and specialized cell types (e.g., pollen tubes, root hairs). Here, we describe a unicellular green alga, Penium margaritaceum (Penium), which can serve as a valuable model organism for understanding cell expansion and the underlying mechanics of the cell wall in a single plant cell.

Original language	English
Title of host publication	Plant cell expansion : methods and protocols
Editors	José M. Estevez
Number of pages	21
Publisher	Springer
Publication date	2015
Pages	1-21
Chapter	1
ISBN (Print)	978-1-4939-1901-7
ISBN (Electronic)	978-1-4939-1902-4
DOIs	https://doi.org/10.1007/978-1-4939-1902-4_1
Publication status	Published - 2015

Series	Methods in Molecular Biology
Volume	1242
ISSN	1064-3745

Keywords

Cell expansion
Confocal laser scanning microscopy
Isolation of pure cell wall
Live cell labeling
Manipulation of cell wall
Penium margaritaceum
Variable pressure scanning electron microscopy

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10.1007/978-1-4939-1902-4_1

Cite this

Rydahl, M. G., Fangel, J. U., Mikkelsen, M. D., Johansen, I. E., Andreas, A., Harholt, J., Ulvskov, P., Jørgensen, B., Domozych, D. S., & Willats, W. G. T. (2015). Penium margaritaceum as a model organism for cell wall analysis of expanding plant cells. In J. M. Estevez (Ed.), Plant cell expansion: methods and protocols (pp. 1-21). Springer. Methods in Molecular Biology Vol. 1242 https://doi.org/10.1007/978-1-4939-1902-4_1

Penium margaritaceum as a model organism for cell wall analysis of expanding plant cells. / Rydahl, Maja Gro; Fangel, Jonatan Ulrik; Mikkelsen, Maria Dalgaard et al.

Plant cell expansion: methods and protocols. ed. / José M. Estevez. Springer, 2015. p. 1-21 (Methods in Molecular Biology, Vol. 1242).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Rydahl, MG, Fangel, JU, Mikkelsen, MD, Johansen, IE, Andreas, A, Harholt, J, Ulvskov, P , Jørgensen, B, Domozych, DS & Willats, WGT 2015, Penium margaritaceum as a model organism for cell wall analysis of expanding plant cells. in JM Estevez (ed.), Plant cell expansion: methods and protocols. Springer, Methods in Molecular Biology, vol. 1242, pp. 1-21. https://doi.org/10.1007/978-1-4939-1902-4_1

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abstract = "The growth of a plant cell encompasses a complex set of subcellular components interacting in a highly coordinated fashion. Ultimately, these activities create specific cell wall structural domains that regulate the prime force of expansion, internally generated turgor pressure. The precise organization of the polymeric networks of the cell wall around the protoplast also contributes to the direction of growth, the shape of the cell, and the proper positioning of the cell in a tissue. In essence, plant cell expansion represents the foundation of development. Most studies of plant cell expansion have focused primarily upon late divergent multicellular land plants and specialized cell types (e.g., pollen tubes, root hairs). Here, we describe a unicellular green alga, Penium margaritaceum (Penium), which can serve as a valuable model organism for understanding cell expansion and the underlying mechanics of the cell wall in a single plant cell.",

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Penium margaritaceum as a model organism for cell wall analysis of expanding plant cells

Abstract

Keywords

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