para-Aminothiophenol Radical Reaction-Functionalized Gold Nanoprobe for One-to-All Detection of Five Reactive Oxygen Species in Vivo

TY - JOUR

T1 - para-Aminothiophenol Radical Reaction-Functionalized Gold Nanoprobe for One-to-All Detection of Five Reactive Oxygen Species in Vivo

AU - Cui, Kai

AU - Fan, Chenchen

AU - Chen, Gaoxian

AU - Qiu, Yuanyuan

AU - Li, Mingwang

AU - Lin, Miao

AU - Wan, Jian Bo

AU - Cai, Changsi

AU - Xiao, Zeyu

PY - 2018

Y1 - 2018

N2 - Five major reactive oxygen species (ROS) are generated in diseases including H2O2, •OH, O2 •-, ROO•, and 1O2. Simultaneous detection of the five ROS with a single probe is crucial for a comprehensive understanding of the development and progression of many diseases, such as cancer and inflammatory diseases. However, currently reported detection systems are limited by targeting one ROS with one probe. This one-to-one detection mode may fail to sufficiently unveil the diseased state. In this study, we achieved simultaneous detection of all the five ROS with one probe (i.e., one-to-all detection), by designing a novel para-aminothiophenol (PATP) and hemin-decorated gold (Au/PATP/Hemin) nanoprobe. The design is principled by our discovery that PATP can react with •OH, O2 •-, ROO•, and 1O2 by a radical oxidative coupling mechanism to form 4,4′-dimercaptoazobenzene (DMAB). The DMAB then elicited strong characteristic surface-enhanced Raman scattering (SERS) peaks at 1142, 1386, and 1432 cm-1 which in turn enables direct detection of •OH, O2 •-, ROO•, and 1O2 and indirect detection of H2O2 by hemin-catalyzed fenton reaction to convert H2O2 into •OH. In two representative ROS-elevated mice models of tumors and allergic dermatitis, the Au/PATP/Hemin nanoprobe demonstrated its robust performance of monitoring tumor development and inflammation progression in a highly sensitive and quantitative manner.

AB - Five major reactive oxygen species (ROS) are generated in diseases including H2O2, •OH, O2 •-, ROO•, and 1O2. Simultaneous detection of the five ROS with a single probe is crucial for a comprehensive understanding of the development and progression of many diseases, such as cancer and inflammatory diseases. However, currently reported detection systems are limited by targeting one ROS with one probe. This one-to-one detection mode may fail to sufficiently unveil the diseased state. In this study, we achieved simultaneous detection of all the five ROS with one probe (i.e., one-to-all detection), by designing a novel para-aminothiophenol (PATP) and hemin-decorated gold (Au/PATP/Hemin) nanoprobe. The design is principled by our discovery that PATP can react with •OH, O2 •-, ROO•, and 1O2 by a radical oxidative coupling mechanism to form 4,4′-dimercaptoazobenzene (DMAB). The DMAB then elicited strong characteristic surface-enhanced Raman scattering (SERS) peaks at 1142, 1386, and 1432 cm-1 which in turn enables direct detection of •OH, O2 •-, ROO•, and 1O2 and indirect detection of H2O2 by hemin-catalyzed fenton reaction to convert H2O2 into •OH. In two representative ROS-elevated mice models of tumors and allergic dermatitis, the Au/PATP/Hemin nanoprobe demonstrated its robust performance of monitoring tumor development and inflammation progression in a highly sensitive and quantitative manner.

U2 - 10.1021/acs.analchem.8b03116

DO - 10.1021/acs.analchem.8b03116

M3 - Journal article

C2 - 30207154

AN - SCOPUS:85054139281

SN - 0003-2700

VL - 90

SP - 12137

EP - 12144

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 20

ER -