Involvement of bacterial quorum-sensing signals in spoilage of bean sprouts

Maria Rasch; Jens Bo Andersen; Kristian Fog Nielsen; Lars Ravn Flodgaard; Henrik Christensen; Michael Givskov; Lone Gram

doi:10.1128/AEM.71.6.3321-3330.2005

Involvement of bacterial quorum-sensing signals in spoilage of bean sprouts

Maria Rasch, Jens Bo Andersen, Kristian Fog Nielsen, Lars Ravn Flodgaard, Henrik Christensen, Michael Givskov, Lone Gram

80 Citations (Scopus)

Abstract

Bacterial communication signals, acylated homoserine lactones (AHLs), were extracted from samples of commercial bean sprouts undergoing soft-rot spoilage. Bean sprouts produced in the laboratory did not undergo soft-rot spoilage and did not contain AHLs or AHL-producing bacteria, although the bacterial population reached levels similar to those in the commercial sprouts, 10(8) to 10(9) CFU/g. AHL-producing bacteria (Enterobacteriaceae and pseudomonads) were isolated from commercial sprouts, and strains that were both proteolytic and pectinolytic were capable of causing soft-rot spoilage in bean sprouts. Thin-layer chromatography and liquid chromatography-high-resolution mass spectrometry revealed the presence of N-3-oxo-hexanoyl-l-homoserine lactone in spoiled bean sprouts and in extracts from pure cultures of bacteria. During normal spoilage, the pH of the sprouts increased due to proteolytic activity, and the higher pH probably facilitated the activity of pectate lyase. The AHL synthetase gene (I gene) from a spoilage Pectobacterium was cloned, sequenced, and inactivated in the parent strain. The predicted amino acid sequence showed 97% homology to HslI and CarI in Erwinia carotovora. Spoilage of laboratory bean sprouts inoculated with the AHL-negative mutant was delayed compared to sprouts inoculated with the wild type, and the AHL-negative mutant did not cause the pH to rise. Compared to the wild-type strain, the AHL-negative mutant had significantly reduced protease and pectinase activities and was negative in an iron chelation (siderophore) assay. This is the first study demonstrating AHL regulation of iron chelation in Enterobacteriaceae. The present study clearly demonstrates that the bacterial spoilage of some food products is influenced by quorum-sensing-regulated phenotypes, and understanding these processes may be useful in the development of novel food preservation additives that specifically block the quorum-sensing systems.

Original language	English
Journal	Applied and Environmental Microbiology
Volume	71
Issue number	6
Pages (from-to)	3321-30
Number of pages	9
ISSN	0099-2240
DOIs	https://doi.org/10.1128/AEM.71.6.3321-3330.2005
Publication status	Published - 2005

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10.1128/AEM.71.6.3321-3330.2005

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@article{c5a95d90fcee11ddb219000ea68e967b,

title = "Involvement of bacterial quorum-sensing signals in spoilage of bean sprouts",

abstract = "Bacterial communication signals, acylated homoserine lactones (AHLs), were extracted from samples of commercial bean sprouts undergoing soft-rot spoilage. Bean sprouts produced in the laboratory did not undergo soft-rot spoilage and did not contain AHLs or AHL-producing bacteria, although the bacterial population reached levels similar to those in the commercial sprouts, 10(8) to 10(9) CFU/g. AHL-producing bacteria (Enterobacteriaceae and pseudomonads) were isolated from commercial sprouts, and strains that were both proteolytic and pectinolytic were capable of causing soft-rot spoilage in bean sprouts. Thin-layer chromatography and liquid chromatography-high-resolution mass spectrometry revealed the presence of N-3-oxo-hexanoyl-l-homoserine lactone in spoiled bean sprouts and in extracts from pure cultures of bacteria. During normal spoilage, the pH of the sprouts increased due to proteolytic activity, and the higher pH probably facilitated the activity of pectate lyase. The AHL synthetase gene (I gene) from a spoilage Pectobacterium was cloned, sequenced, and inactivated in the parent strain. The predicted amino acid sequence showed 97% homology to HslI and CarI in Erwinia carotovora. Spoilage of laboratory bean sprouts inoculated with the AHL-negative mutant was delayed compared to sprouts inoculated with the wild type, and the AHL-negative mutant did not cause the pH to rise. Compared to the wild-type strain, the AHL-negative mutant had significantly reduced protease and pectinase activities and was negative in an iron chelation (siderophore) assay. This is the first study demonstrating AHL regulation of iron chelation in Enterobacteriaceae. The present study clearly demonstrates that the bacterial spoilage of some food products is influenced by quorum-sensing-regulated phenotypes, and understanding these processes may be useful in the development of novel food preservation additives that specifically block the quorum-sensing systems.",

author = "Maria Rasch and Andersen, {Jens Bo} and Nielsen, {Kristian Fog} and Flodgaard, {Lars Ravn} and Henrik Christensen and Michael Givskov and Lone Gram",

note = "Keywords: 4-Butyrolactone; Bacterial Proteins; Colony Count, Microbial; DNA, Bacterial; Enterobacteriaceae; Fabaceae; Gene Expression Regulation, Bacterial; Gram-Negative Bacteria; Molecular Sequence Data; Mutation; Phenotype; Pseudomonas; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Signal Transduction; Vibrionaceae",

year = "2005",

doi = "10.1128/AEM.71.6.3321-3330.2005",

language = "English",

volume = "71",

pages = "3321--30",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "6",

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TY - JOUR

T1 - Involvement of bacterial quorum-sensing signals in spoilage of bean sprouts

AU - Rasch, Maria

AU - Andersen, Jens Bo

AU - Nielsen, Kristian Fog

AU - Flodgaard, Lars Ravn

AU - Christensen, Henrik

AU - Givskov, Michael

AU - Gram, Lone

N1 - Keywords: 4-Butyrolactone; Bacterial Proteins; Colony Count, Microbial; DNA, Bacterial; Enterobacteriaceae; Fabaceae; Gene Expression Regulation, Bacterial; Gram-Negative Bacteria; Molecular Sequence Data; Mutation; Phenotype; Pseudomonas; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Signal Transduction; Vibrionaceae

PY - 2005

Y1 - 2005

N2 - Bacterial communication signals, acylated homoserine lactones (AHLs), were extracted from samples of commercial bean sprouts undergoing soft-rot spoilage. Bean sprouts produced in the laboratory did not undergo soft-rot spoilage and did not contain AHLs or AHL-producing bacteria, although the bacterial population reached levels similar to those in the commercial sprouts, 10(8) to 10(9) CFU/g. AHL-producing bacteria (Enterobacteriaceae and pseudomonads) were isolated from commercial sprouts, and strains that were both proteolytic and pectinolytic were capable of causing soft-rot spoilage in bean sprouts. Thin-layer chromatography and liquid chromatography-high-resolution mass spectrometry revealed the presence of N-3-oxo-hexanoyl-l-homoserine lactone in spoiled bean sprouts and in extracts from pure cultures of bacteria. During normal spoilage, the pH of the sprouts increased due to proteolytic activity, and the higher pH probably facilitated the activity of pectate lyase. The AHL synthetase gene (I gene) from a spoilage Pectobacterium was cloned, sequenced, and inactivated in the parent strain. The predicted amino acid sequence showed 97% homology to HslI and CarI in Erwinia carotovora. Spoilage of laboratory bean sprouts inoculated with the AHL-negative mutant was delayed compared to sprouts inoculated with the wild type, and the AHL-negative mutant did not cause the pH to rise. Compared to the wild-type strain, the AHL-negative mutant had significantly reduced protease and pectinase activities and was negative in an iron chelation (siderophore) assay. This is the first study demonstrating AHL regulation of iron chelation in Enterobacteriaceae. The present study clearly demonstrates that the bacterial spoilage of some food products is influenced by quorum-sensing-regulated phenotypes, and understanding these processes may be useful in the development of novel food preservation additives that specifically block the quorum-sensing systems.

AB - Bacterial communication signals, acylated homoserine lactones (AHLs), were extracted from samples of commercial bean sprouts undergoing soft-rot spoilage. Bean sprouts produced in the laboratory did not undergo soft-rot spoilage and did not contain AHLs or AHL-producing bacteria, although the bacterial population reached levels similar to those in the commercial sprouts, 10(8) to 10(9) CFU/g. AHL-producing bacteria (Enterobacteriaceae and pseudomonads) were isolated from commercial sprouts, and strains that were both proteolytic and pectinolytic were capable of causing soft-rot spoilage in bean sprouts. Thin-layer chromatography and liquid chromatography-high-resolution mass spectrometry revealed the presence of N-3-oxo-hexanoyl-l-homoserine lactone in spoiled bean sprouts and in extracts from pure cultures of bacteria. During normal spoilage, the pH of the sprouts increased due to proteolytic activity, and the higher pH probably facilitated the activity of pectate lyase. The AHL synthetase gene (I gene) from a spoilage Pectobacterium was cloned, sequenced, and inactivated in the parent strain. The predicted amino acid sequence showed 97% homology to HslI and CarI in Erwinia carotovora. Spoilage of laboratory bean sprouts inoculated with the AHL-negative mutant was delayed compared to sprouts inoculated with the wild type, and the AHL-negative mutant did not cause the pH to rise. Compared to the wild-type strain, the AHL-negative mutant had significantly reduced protease and pectinase activities and was negative in an iron chelation (siderophore) assay. This is the first study demonstrating AHL regulation of iron chelation in Enterobacteriaceae. The present study clearly demonstrates that the bacterial spoilage of some food products is influenced by quorum-sensing-regulated phenotypes, and understanding these processes may be useful in the development of novel food preservation additives that specifically block the quorum-sensing systems.

U2 - 10.1128/AEM.71.6.3321-3330.2005

DO - 10.1128/AEM.71.6.3321-3330.2005

M3 - Journal article

C2 - 15933035

SN - 0099-2240

VL - 71

SP - 3321

EP - 3330

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 6

ER -

Involvement of bacterial quorum-sensing signals in spoilage of bean sprouts

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