Investigation of the pathogenesis of Enterococcus cecorum after intravenous, intratracheal or oral experimental infections of broilers and broiler breeders.

Ida  Thoefner; Jens Peter Christensen

Investigation of the pathogenesis of Enterococcus cecorum after intravenous, intratracheal or oral experimental infections of broilers and broiler breeders.

Abstract

Enterococcus cecorum outbreaks has emerged in broiler flocks all over Europe, with considerable economical and animal welfare
consequences. The infection has a broad range of manifestations (e.g. pericarditis, arthritis, osteomyelitis, and sepsis) leading to increased
mortality. Currently, little is known concerning transmission and pathogenesis in broilers and their parents.
Consequently, a set of experimental E. cecorum infections were conducted in broiler breeders and broilers. The bacteria was inoculated at two
doses using intravenous (IV), intratracheal (IT) and oral (PO) route of infection in both bird types. Full post mortem (PM) and bacteriological
investigation 3, and 10 days after infection were performed. Bacteriology was performed daily on the eggs from the parent birds.
After IV infection of the broiler breeders (high dose) a pronounced egg drop and decreased appetite was observed. Despite very mild or no lesions
the majority of the high dose group demonstrated low grade bacteraemia at both time points. In the low dose group no lesions was observed and
only 1 bird had bacteraemia. No clinical signs, PM lesion or positive bacterial re-isolation was observed. No E. cecorum could be detected from the
egg content.
In the broilers, all birds in the IV high dose group died within 12h of peracute sepsis, despite massive bacteraemia very few lesions was observed.
The PM findings in the low dose IV group resembled the field outbreaks, with severe pericarditis as the major manifestation. The IT and PO
infections are on-going.
The preliminary results indicate a clear age-related difference in susceptibility of E. cecorum infections, also suggesting dose dependant and
transmission route differences.. There is no indication of a genuine vertical transmission to the offspring via the e

Original language	English
Publication date	Oct 2016
Publication status	Published - Oct 2016
Event	VETPATH 2016 - Monash University, Prato, Italy Duration: 11 Oct 2016 → 14 Oct 2016

Conference

Conference	VETPATH 2016
Location	Monash University
Country/Territory	Italy
City	Prato
Period	11/10/2016 → 14/10/2016

Access to Document

http://vetpath2016.org/assets/Vetpath-2016-Abstracts.pdf

VETPATH 2016
Thøfner, I. (Speaker)
11 Oct 2016 → 14 Oct 2016
Activity: Participating in or organising an event types › Organisation of and participation in conference

Cite this

@conference{52f594dfe61c48aeb874d44f659fbf61,

title = "Investigation of the pathogenesis of Enterococcus cecorum after intravenous, intratracheal or oral experimental infections of broilers and broiler breeders.",

abstract = "Enterococcus cecorum outbreaks has emerged in broiler flocks all over Europe, with considerable economical and animal welfareconsequences. The infection has a broad range of manifestations (e.g. pericarditis, arthritis, osteomyelitis, and sepsis) leading to increasedmortality. Currently, little is known concerning transmission and pathogenesis in broilers and their parents.Consequently, a set of experimental E. cecorum infections were conducted in broiler breeders and broilers. The bacteria was inoculated at twodoses using intravenous (IV), intratracheal (IT) and oral (PO) route of infection in both bird types. Full post mortem (PM) and bacteriologicalinvestigation 3, and 10 days after infection were performed. Bacteriology was performed daily on the eggs from the parent birds.After IV infection of the broiler breeders (high dose) a pronounced egg drop and decreased appetite was observed. Despite very mild or no lesionsthe majority of the high dose group demonstrated low grade bacteraemia at both time points. In the low dose group no lesions was observed andonly 1 bird had bacteraemia. No clinical signs, PM lesion or positive bacterial re-isolation was observed. No E. cecorum could be detected from theegg content.In the broilers, all birds in the IV high dose group died within 12h of peracute sepsis, despite massive bacteraemia very few lesions was observed.The PM findings in the low dose IV group resembled the field outbreaks, with severe pericarditis as the major manifestation. The IT and POinfections are on-going.The preliminary results indicate a clear age-related difference in susceptibility of E. cecorum infections, also suggesting dose dependant andtransmission route differences.. There is no indication of a genuine vertical transmission to the offspring via the e",

author = "Ida Thoefner and Christensen, {Jens Peter}",

year = "2016",

month = oct,

language = "English",

note = "VETPATH 2016 ; Conference date: 11-10-2016 Through 14-10-2016",

}

TY - CONF

T1 - Investigation of the pathogenesis of Enterococcus cecorum after intravenous, intratracheal or oral experimental infections of broilers and broiler breeders.

AU - Thoefner, Ida

AU - Christensen, Jens Peter

PY - 2016/10

Y1 - 2016/10

N2 - Enterococcus cecorum outbreaks has emerged in broiler flocks all over Europe, with considerable economical and animal welfareconsequences. The infection has a broad range of manifestations (e.g. pericarditis, arthritis, osteomyelitis, and sepsis) leading to increasedmortality. Currently, little is known concerning transmission and pathogenesis in broilers and their parents.Consequently, a set of experimental E. cecorum infections were conducted in broiler breeders and broilers. The bacteria was inoculated at twodoses using intravenous (IV), intratracheal (IT) and oral (PO) route of infection in both bird types. Full post mortem (PM) and bacteriologicalinvestigation 3, and 10 days after infection were performed. Bacteriology was performed daily on the eggs from the parent birds.After IV infection of the broiler breeders (high dose) a pronounced egg drop and decreased appetite was observed. Despite very mild or no lesionsthe majority of the high dose group demonstrated low grade bacteraemia at both time points. In the low dose group no lesions was observed andonly 1 bird had bacteraemia. No clinical signs, PM lesion or positive bacterial re-isolation was observed. No E. cecorum could be detected from theegg content.In the broilers, all birds in the IV high dose group died within 12h of peracute sepsis, despite massive bacteraemia very few lesions was observed.The PM findings in the low dose IV group resembled the field outbreaks, with severe pericarditis as the major manifestation. The IT and POinfections are on-going.The preliminary results indicate a clear age-related difference in susceptibility of E. cecorum infections, also suggesting dose dependant andtransmission route differences.. There is no indication of a genuine vertical transmission to the offspring via the e

AB - Enterococcus cecorum outbreaks has emerged in broiler flocks all over Europe, with considerable economical and animal welfareconsequences. The infection has a broad range of manifestations (e.g. pericarditis, arthritis, osteomyelitis, and sepsis) leading to increasedmortality. Currently, little is known concerning transmission and pathogenesis in broilers and their parents.Consequently, a set of experimental E. cecorum infections were conducted in broiler breeders and broilers. The bacteria was inoculated at twodoses using intravenous (IV), intratracheal (IT) and oral (PO) route of infection in both bird types. Full post mortem (PM) and bacteriologicalinvestigation 3, and 10 days after infection were performed. Bacteriology was performed daily on the eggs from the parent birds.After IV infection of the broiler breeders (high dose) a pronounced egg drop and decreased appetite was observed. Despite very mild or no lesionsthe majority of the high dose group demonstrated low grade bacteraemia at both time points. In the low dose group no lesions was observed andonly 1 bird had bacteraemia. No clinical signs, PM lesion or positive bacterial re-isolation was observed. No E. cecorum could be detected from theegg content.In the broilers, all birds in the IV high dose group died within 12h of peracute sepsis, despite massive bacteraemia very few lesions was observed.The PM findings in the low dose IV group resembled the field outbreaks, with severe pericarditis as the major manifestation. The IT and POinfections are on-going.The preliminary results indicate a clear age-related difference in susceptibility of E. cecorum infections, also suggesting dose dependant andtransmission route differences.. There is no indication of a genuine vertical transmission to the offspring via the e

M3 - Poster

T2 - VETPATH 2016

Y2 - 11 October 2016 through 14 October 2016

ER -

Investigation of the pathogenesis of Enterococcus cecorum after intravenous, intratracheal or oral experimental infections of broilers and broiler breeders.

Abstract

Conference

Access to Document

Fingerprint

Activities

VETPATH 2016

Cite this