Investigation of free seleno-amino acids in extra-virgin olive oil by mixed mode solid phase extraction cleanup and enantioselective hydrophilic interaction liquid chromatography-tandem mass spectrometry

Susy Piovesana; Carmela Maria Montone; Michela Antonelli; Chiara Cavaliere; Giorgia La Barbera; Silvia Canepari; Roberto Samperi; Aldo Laganà; Anna Laura Capriotti

doi:10.1016/j.foodchem.2018.11.053

Investigation of free seleno-amino acids in extra-virgin olive oil by mixed mode solid phase extraction cleanup and enantioselective hydrophilic interaction liquid chromatography-tandem mass spectrometry

Susy Piovesana, Carmela Maria Montone, Michela Antonelli, Chiara Cavaliere^*, Giorgia La Barbera, Silvia Canepari, Roberto Samperi, Aldo Laganà, Anna Laura Capriotti

^*Corresponding author for this work

4 Citations (Scopus)

Abstract

An analytical method for determining seleno-methionine (SeMet), methyl-seleno-cysteine and seleno-cystine in extra-virgin olive oil (EVOO) was developed and validated. EVOO sample (15 g) was diluted with hexane, extracted with methanol/water 80:20 (v/v), and cleaned up by a reversed phase/strong cation exchange solid phase extraction. Analysis was performed by chiral hydrophilic interaction liquid chromatography-tandem mass spectrometry. Process efficiency ranged between 49 and 97% and trueness between 87 and 126%, with intermediate precision, expressed as standard deviation, lower than 10%. Method detection limits (MDLs) and method quantification limits (MQLs) were lower than 1 μg kg⁻¹. Thirty-two EVOO samples from different Italian regions were analyzed for both total Se and single seleno-amino acids determination. Only L-SeMet was found at level MQL (0.2 μg kg⁻¹)-1.42 μg kg⁻¹ in ten samples, while total Se was in the range of MDL-9.1 μg kg⁻¹. Concentration of L-SeMet (5–6% of total Se) and total Se correlated very well to each other (R² = 0.995).

Original language	English
Journal	Food Chemistry
Volume	278
Pages (from-to)	17-25
Number of pages	9
ISSN	0308-8146
DOIs	https://doi.org/10.1016/j.foodchem.2018.11.053
Publication status	Published - 2019
Externally published	Yes

Keywords

Enantioselective high performance liquid chromatography (eHPLC)
Extra virgin olive oil
Mass spectrometry
Seleno-amino acids
Total Selenium

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10.1016/j.foodchem.2018.11.053

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Piovesana, S., Montone, C. M., Antonelli, M., Cavaliere, C., La Barbera, G., Canepari, S., Samperi, R., Laganà, A., & Capriotti, A. L. (2019). Investigation of free seleno-amino acids in extra-virgin olive oil by mixed mode solid phase extraction cleanup and enantioselective hydrophilic interaction liquid chromatography-tandem mass spectrometry. Food Chemistry, 278, 17-25. https://doi.org/10.1016/j.foodchem.2018.11.053

Investigation of free seleno-amino acids in extra-virgin olive oil by mixed mode solid phase extraction cleanup and enantioselective hydrophilic interaction liquid chromatography-tandem mass spectrometry. / Piovesana, Susy; Montone, Carmela Maria; Antonelli, Michela et al.
In: Food Chemistry, Vol. 278, 2019, p. 17-25.

Research output: Contribution to journal › Journal article › Research › peer-review

Piovesana, S, Montone, CM, Antonelli, M, Cavaliere, C, La Barbera, G, Canepari, S, Samperi, R, Laganà, A & Capriotti, AL 2019, 'Investigation of free seleno-amino acids in extra-virgin olive oil by mixed mode solid phase extraction cleanup and enantioselective hydrophilic interaction liquid chromatography-tandem mass spectrometry', Food Chemistry, vol. 278, pp. 17-25. https://doi.org/10.1016/j.foodchem.2018.11.053

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title = "Investigation of free seleno-amino acids in extra-virgin olive oil by mixed mode solid phase extraction cleanup and enantioselective hydrophilic interaction liquid chromatography-tandem mass spectrometry",

abstract = "An analytical method for determining seleno-methionine (SeMet), methyl-seleno-cysteine and seleno-cystine in extra-virgin olive oil (EVOO) was developed and validated. EVOO sample (15 g) was diluted with hexane, extracted with methanol/water 80:20 (v/v), and cleaned up by a reversed phase/strong cation exchange solid phase extraction. Analysis was performed by chiral hydrophilic interaction liquid chromatography-tandem mass spectrometry. Process efficiency ranged between 49 and 97% and trueness between 87 and 126%, with intermediate precision, expressed as standard deviation, lower than 10%. Method detection limits (MDLs) and method quantification limits (MQLs) were lower than 1 μg kg−1. Thirty-two EVOO samples from different Italian regions were analyzed for both total Se and single seleno-amino acids determination. Only L-SeMet was found at level MQL (0.2 μg kg−1)-1.42 μg kg−1 in ten samples, while total Se was in the range of MDL-9.1 μg kg−1. Concentration of L-SeMet (5–6% of total Se) and total Se correlated very well to each other (R2 = 0.995).",

keywords = "Enantioselective high performance liquid chromatography (eHPLC), Extra virgin olive oil, Mass spectrometry, Seleno-amino acids, Total Selenium",

author = "Susy Piovesana and Montone, {Carmela Maria} and Michela Antonelli and Chiara Cavaliere and {La Barbera}, Giorgia and Silvia Canepari and Roberto Samperi and Aldo Lagan{\`a} and Capriotti, {Anna Laura}",

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AU - Piovesana, Susy

AU - Montone, Carmela Maria

AU - Antonelli, Michela

AU - Cavaliere, Chiara

AU - La Barbera, Giorgia

AU - Canepari, Silvia

AU - Samperi, Roberto

AU - Laganà, Aldo

AU - Capriotti, Anna Laura

N1 - (Ekstern)

PY - 2019

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N2 - An analytical method for determining seleno-methionine (SeMet), methyl-seleno-cysteine and seleno-cystine in extra-virgin olive oil (EVOO) was developed and validated. EVOO sample (15 g) was diluted with hexane, extracted with methanol/water 80:20 (v/v), and cleaned up by a reversed phase/strong cation exchange solid phase extraction. Analysis was performed by chiral hydrophilic interaction liquid chromatography-tandem mass spectrometry. Process efficiency ranged between 49 and 97% and trueness between 87 and 126%, with intermediate precision, expressed as standard deviation, lower than 10%. Method detection limits (MDLs) and method quantification limits (MQLs) were lower than 1 μg kg−1. Thirty-two EVOO samples from different Italian regions were analyzed for both total Se and single seleno-amino acids determination. Only L-SeMet was found at level MQL (0.2 μg kg−1)-1.42 μg kg−1 in ten samples, while total Se was in the range of MDL-9.1 μg kg−1. Concentration of L-SeMet (5–6% of total Se) and total Se correlated very well to each other (R2 = 0.995).

AB - An analytical method for determining seleno-methionine (SeMet), methyl-seleno-cysteine and seleno-cystine in extra-virgin olive oil (EVOO) was developed and validated. EVOO sample (15 g) was diluted with hexane, extracted with methanol/water 80:20 (v/v), and cleaned up by a reversed phase/strong cation exchange solid phase extraction. Analysis was performed by chiral hydrophilic interaction liquid chromatography-tandem mass spectrometry. Process efficiency ranged between 49 and 97% and trueness between 87 and 126%, with intermediate precision, expressed as standard deviation, lower than 10%. Method detection limits (MDLs) and method quantification limits (MQLs) were lower than 1 μg kg−1. Thirty-two EVOO samples from different Italian regions were analyzed for both total Se and single seleno-amino acids determination. Only L-SeMet was found at level MQL (0.2 μg kg−1)-1.42 μg kg−1 in ten samples, while total Se was in the range of MDL-9.1 μg kg−1. Concentration of L-SeMet (5–6% of total Se) and total Se correlated very well to each other (R2 = 0.995).

KW - Enantioselective high performance liquid chromatography (eHPLC)

KW - Extra virgin olive oil

KW - Mass spectrometry

KW - Seleno-amino acids

KW - Total Selenium

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Investigation of free seleno-amino acids in extra-virgin olive oil by mixed mode solid phase extraction cleanup and enantioselective hydrophilic interaction liquid chromatography-tandem mass spectrometry

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Keywords

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