Abstract
An analytical method for determining seleno-methionine, methyl-seleno-cysteine, and seleno-cystine in wheat bran was developed and validated. Four different extraction procedures were evaluated to simultaneously extract endogenous free and conjugated seleno-amino acids in wheat bran in order to select the best extraction protocol in terms of seleno amino acid quantitation. The extracted samples were subjected to a clean-up by a reversed phase/strong cation exchange solid-phase extraction and analyzed by chiral hydrophilic interaction liquid chromatography-tandem mass spectrometry. The optimized extraction protocol was employed to validate the methodology. Process efficiency ranged from 58 to 112% and trueness from 73 to 98%. Limit of detection and limit of quantification were lower than 1 ng/g. Four wheat bran samples were analyzed for both total Se and single seleno-amino acids determination. The results showed that Se- seleno-methyl-lselenocysteine was the major seleno-amino acid in wheat bran while seleno-methionine and seleno-cysteine were both minor species.
| Original language | English |
|---|---|
| Journal | Journal of Separation Science |
| Volume | 42 |
| Issue number | 10 |
| Pages (from-to) | 1938-1947 |
| Number of pages | 10 |
| ISSN | 1615-9306 |
| DOIs | |
| Publication status | Published - 2019 |
| Externally published | Yes |
Keywords
- Enantioselective high-performance liquid chromatography
- Mass spectrometry
- Seleno-amino acids
- Total selenium
- Wheat bran